Full description
The developmental profiles of 19,611 barley gene transcripts were precisely defined in the purified tissues and in whole grain during the first 24 hours of germination by RNA-seq. Spatial and temporal patterns of transcription were validated against well-defined data on enzyme activities both in whole grain and in isolated tissues. Transcript profiles of genes involved in mitochondrial assembly and function were used to validate the very early stages of germination. The data will be broadly applicable for the interrogation of co-expression and differential expression patterns and for the identification of transcription factors that are important in the early stages of grain and seed germination.
Created: 2017
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Isolation of Tissues and Preservation of RNA from Intact, Germinated Barley Grain
doi :
10.1111/tpj.13600
Article Citation: Betts, N. S., Berkowitz, O., Liu, R., Collins, H. M., Skadhauge, B., Dockter, C., Burton, R. A., Whelan, J. and Fincher, G. B. (2017), Isolation of Tissues and Preservation of RNA from Intact, Germinated Barley Grain. Plant J. Accepted Author Manuscript. doi:10.1111/tpj.13600
La Trobe University Genomics Platform
uri :
http://www.latrobe.edu.au/genomics
The La Trobe University Genomics Platform provides investigators with the latest in next-generation sequencing equipment and complementary bioinformatics assistance. We facilitate wide-ranging research projects requiring highly detailed global DNA and RNA sequence analysis. The Genomics Platform is part of the La Trobe Research Platforms and is available to investigators both internal and external to La Trobe University.
La Trobe University RNAseq Database
doi :
10.4225/22/592fa09c2f852
- DOI : 10.4225/22/5934B5F7E6045
