Total RNA of mouse brain of E15.5

Brief description

A pregnant mouse was bred under a 12-hour light and 12-hour dark cycle with access to unlimited food and water. Embryos were removed after 15.5 days (E15.5) of pregnancy. The developing whole brain was dissected from one of the embryos for total RNA extraction. Total RNA was isolated from a whole brain dissected from an E15.5 embryo of C57BL/6 background using TRIzol reagent (Invitrogen) according to the manufacturer’s protocol. Small RNAs with sizes ranging from 16-30nt were isolated from 10μg total RNA using polyacrylamide gel electrophoresis. The complementary small RNA library was constructed using the Small RNA Sample Prep Kit version 1.0 (Illumina) according to the manufacturer's protocol with 5’-GTTCAGAGTT CTACAGTCCG ACGATC-3’ and 5’- TCGTATGCCG TCTTCTGCTT GT-3’ adapters at the 5’ and 3’ ends, respectively.