Microsatellite multiplexes for parentage analysis and species discrimination in coral trout (NERP TE 8.3, JCU)

Brief description

This dataset demonstrates the suitability of microsatellite markers to discriminate between species of coral trout (Plectropomus spp.) and identify parent-offspring relationships in natural populations. A total of 285 adult P. leopardus collected from the Capricorn Bunkers and 285 adult P. maculatus from the Keppel islands were genotyped at 25 microsatellite loci. Each locus was also tested on 7 additional species within the genus. The multiplex PCRs developed here provide a reliable and cost-effective strategy to investigate the evolutionary and ecological dynamics of coral trout. Microsatellite loci are commonly used in ecology to measure genetic variability within and among populations. Their high allelic diversity and relative ease of development also make them ideal for individual genotyping to assist in species identification, to uniquely identify individuals and to infer phylogenetic or genealogical relationships. In the marine environment, these genetic tools may be the only means to measure important ecological processes such as larval dispersal, adult migrations and reproductive success. However, applying these methods accurately can require numerous, highly polymorphic markers and optimised PCR multiplexes can maximise the cost-effectiveness of using microsatellites. Methods: Microsatellite loci were identified from a microsatellite-enriched cloning library developed for P. maculatus and 454 pyrosequencing libraries for both P. leopardus and P. maculatus. The isolation of microsatellite loci, their integration to multiplex PCR and suitability for species discrimination can be found in Harrison et al. (2014). Limitations: This datasets is intended for the purpose of describing the suitability of microsatellite markers for species discrimination and parentage analysis in P. leopardus and P. maculatus. Format: Excel file includes: 1. Microsatellite data - Individual identifier - Species name - 25 codominant microsatellite loci 2. Description of 25 microsatellite loci - Locus name - Forward primer sequence - Reverse primer sequence - Genbank digital repository accession number - Library microsatellite were isolated from - PCR multiplex dye label - Repeat motif - PCR reaction concentration - Size range 3. Locus characteristics for P. leopardus and P. maculatus - Locus name - Number of alleles per locus - Observed heterozygosity - Expected heterozygosity - Hardy-Weinberg equilibrium - Probability of exclusion - Cumulative probability of exclusion 4. Locus transferability inPlectropomus spp. - Locus name - Number of samples tested - Number of observed alleles - Size range References: Harrison HB, Feldheim KA, Jones GP, Ma K, Mansour H, Perumal S, Williamson DH and Berumen ML (2014). Validation of microsatellite multiplexes for parentage analysis and species discrimination in two hybridizing species of coral reef fish (Plectropomus spp., Serranidae”. Ecology and Evolution, DOI: 10.1002/ece3.1002