Data

Amplicon sequencing of Cyp51 alleles from Pyrenophora teres using the Oxford Nanopore Technologies MinION (version 1)

Curtin University
Katherine Zulak (Aggregated by)
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.25917/ckw7-2m51&rft.title=Amplicon sequencing of Cyp51 alleles from Pyrenophora teres using the Oxford Nanopore Technologies MinION (version 1)&rft.identifier=10.25917/ckw7-2m51&rft.publisher=Curtin University&rft.description=Data description: Samples are barley (Hordeum vulgare) leaves infected with Pyrenophora teres collected from paddocks in Western Australia. The azole fungicide target gene Cyp51 was amplified from the samples using unique molecular identifiers according to Zulak et al., (2024) Scientific Reports 14:1 6285. Amplicons were subsequently sequenced with the Oxford Nanopore Technologies MinION sequencer using the Native Barcoding Kit V14 and the MIN114 flow cell. The resulting data file is a .fastq file containing sequence information. Collected under CCDM Project A3.4. Number of files/types: 1 .zip file containing 286 .fastq.gz filesGeographic information (if relevant): Western Australia Keywords: sequences, genes, fungal   Please note this dataset has been updated by the version linked below.&rft.creator=Katherine Zulak&rft.date=2025&rft_rights=&rft_subject=Plant pathology&rft_subject=Plant biology&rft_subject=BIOLOGICAL SCIENCES&rft.type=dataset&rft.language=English Access the data
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Data description: Samples are barley (Hordeum vulgare) leaves infected with Pyrenophora teres collected from paddocks in Western Australia. The azole fungicide target gene Cyp51 was amplified from the samples using unique molecular identifiers according to Zulak et al., (2024) Scientific Reports 14:1 6285. Amplicons were subsequently sequenced with the Oxford Nanopore Technologies MinION sequencer using the Native Barcoding Kit V14 and the MIN114 flow cell. The resulting data file is a .fastq file containing sequence information.

Collected under CCDM Project A3.4.

Number of files/types: 1 .zip file containing 286 .fastq.gz files
Geographic information (if relevant): Western Australia
Keywords: sequences, genes, fungal

 

Please note this dataset has been updated by the version linked below.

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Biological Sciences | Plant Biology | Plant Pathology |

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Other Information
Exploiting long read sequencing to detect azole fungicide resistance mutations in Pyrenophora teres using unique molecular identifiers

doi : https://doi.org/10.1038/s41598-024-56801-z

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