Brief description
Camptothecin and Hydrogen peroxide were assessed for ability to induce apoptosis to cancer cells in vitro. Specific molecular probes were used to visualise cell death pathways in vitro by timelapse microscopic imaging, presented as an ND2 'movie' file.
Full description
MCF7 cancer cells that were treated with either Camptothecin or Hydrogen Peroxide and vehicle control were stained with the molecular probes Hoechst, TMRM and CellEvent (Caspase 3/7). Widefield fluorescent microscope timelapse images were collected over a period of 8hrs. The resulting dataset is in the format of an ND2 file (produced in Nikon NIS Elements 3.22.11 and viewable as a 'movie' in NIS Elements).
Notes
To enable improved drug therapies for patients there is an increasing emphasis on understanding the mechanism of drug metabolism in the control of cancer cell proliferation, viability and death . Reliable measurement of cell viability, proliferation, growth inhibition and death is important in screening for drug treatment efficacy in vitro.
Data time period: 2012-01-17 to 2012-01-17
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- Handle : 2100/1344
