Data

WAMSI Node 1.2.3 - An assessment of likely dispersal patterns for marine organisms based on hydrodynamic and population genetic models

Australian Ocean Data Network
CSIRO O&A, Information & Data Centre (Point of contact) CSIRO Oceans & Atmosphere - Hobart (Associated with)
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=https://marlin.csiro.au/geonetwork/srv/eng/catalog.search#/metadata/bc1b3741-e336-5039-e044-00144f7bc0f4&rft.title=WAMSI Node 1.2.3 - An assessment of likely dispersal patterns for marine organisms based on hydrodynamic and population genetic models&rft.identifier=Anzlic Identifier: ANZCW0306008949&rft.publisher=Australian Ocean Data Network&rft.description=Here we predict oceanographic connectivity among four geographical locations in the south west of Western Australia using hydrodynamic modelling of larval dispersal and test these predictions against genetic descriptions of population structure, population boundaries and estimates of larval migration in two co-distributed sea urchin species. Sea urchins are excellent models for characterising marine population connectivity because of their commonness, diversity of larval life histories, ease of sampling, and ecological importance as grazers in coastal benthic habitats. Our study sampled two species, Heliocidaris erythrogramma and Phylocanthius irregularis. H. erythrogramma spawns predominantly in summer and its lecithrotrophic larvae are free swimming for 3-4 days in laboratory observations. In contrast, little is known about spawning time and larval duration in P. irregularis. The south-west corner of Australia is dominated by the Leeuwin current system, the worlds only poleward-flowing western continental boundary current. The Leeuwin current (LC) is particularly strong in the late autumn and winter months and is weaker in the summer. Strong seasonal contrasts in the LC flow pattern make it possible to generate testable predictions about the predominant direction of larvae-mediated gene flow in H. erythrogramma. Comparison of genetic structure with oceanographic model predictions allowed us to make predictions about the possible larval biology of the less well characterised urchin P. irregularis.Progress Code: completedMaintenance and Update Frequency: notPlannedStatement: Two urchin species (Heliocidaris erythrogramma and Phylacanthus irregularis) with ranges encompassing the lower west coast and southern coasts of Western Australia were collected by diving in coastal waters at four locations. Samples were either frozen for later processing in the laboratory or dissected in the field to extract gonads for placement into 95% ethanol. Genetic Testing: A total of 60 H. erythrogramma and 53 P. irregularis specimens were used in the genetic analyses. We obtained sequence from both mitochondrial (mtDNA) and nuclear DNA. For mtDNA analyses, a fragment of the mitochondrial cytochrome oxidase I subunit gene (COI) was amplified via the polymerase chain reaction (PCR) and sequenced using the LCO1490 and HCO2198 primers (Folmer et al., 1994). To obtain nuclear markers we targeted introns of seven single-copy genes, using universal coelomate primers. Sequencing was performed by Macrogen Ltd. (South Korea). Migrate: We used a maximum likelihood approach based on coalescent theory and implemented with the program Migrate-N 3.1.6 to estimate effective population size and migration among sampling sites. Population and search parameters were varied until quantitatively similar maximum likelihood values were returned from successive runs. Modelling: Lagrangian particle dispersal modelling was used to estimate larval connectivity potential among sites. This was nested within a data assimilated hydrodynamic model, BlueLINK with high spatial (0.1 degree) and temporal (6 hours) resolution reconstructing the hydrodynamic history around the Australian region between 1992 and 2006.&rft.creator=Anonymous&rft.date=2011&rft.coverage=westlimit=115; southlimit=-34; eastlimit=115.8; northlimit=-30.2&rft.coverage=westlimit=115; southlimit=-34; eastlimit=115.8; northlimit=-30.2&rft_rights=&rft_rights=Data is made available under a Creative Commons Attribution 4.0 International Licence (http://creativecommons.org/licenses/by/4.0/). Data is supplied 'as is' without any warranty or guarantee except as required by law to be given to you. The data may not be free of error, comprehensive, current or appropriate for your particular purpose. You accept all risk and responsibility for its use. ATTRIBUTION STATEMENT: The dataset [Insert-dataset-name-here] downloaded on [Insert-DD-Mmm-YYYY-here] was provided by CSIRO.&rft_subject=biota&rft_subject=oceans&rft_subject=Coastal Waters (Australia) | West Australia Coast West, WA&rft_subject=Global / Oceans | East Indian Ocean&rft_subject=Global / Oceans | Indian Ocean&rft_subject=WAMSI Node 1 Project 2: Coastal Ecosystem Characterisation&rft_subject=Western Australian Marine Science Institute&rft.type=dataset&rft.language=English Access the data
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Data is made available under a Creative Commons Attribution 4.0 International Licence (http://creativecommons.org/licenses/by/4.0/). Data is supplied 'as is' without any warranty or guarantee except as required by law to be given to you. The data may not be free of error, comprehensive, current or appropriate for your particular purpose. You accept all risk and responsibility for its use. ATTRIBUTION STATEMENT: The dataset [Insert-dataset-name-here] downloaded on [Insert-DD-Mmm-YYYY-here] was provided by CSIRO.

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Here we predict oceanographic connectivity among four geographical locations in the south west of Western Australia using hydrodynamic modelling of larval dispersal and test these predictions against genetic descriptions of population structure, population boundaries and estimates of larval migration in two co-distributed sea urchin species. Sea urchins are excellent models for characterising marine population connectivity because of their commonness, diversity of larval life histories, ease of sampling, and ecological importance as grazers in coastal benthic habitats. Our study sampled two species, Heliocidaris erythrogramma and Phylocanthius irregularis. H. erythrogramma spawns predominantly in summer and its lecithrotrophic larvae are free swimming for 3-4 days in laboratory observations. In contrast, little is known about spawning time and larval duration in P. irregularis. The south-west corner of Australia is dominated by the Leeuwin current system, the worlds only poleward-flowing western continental boundary current. The Leeuwin current (LC) is particularly strong in the late autumn and winter months and is weaker in the summer. Strong seasonal contrasts in the LC flow pattern make it possible to generate testable predictions about the predominant direction of larvae-mediated gene flow in H. erythrogramma. Comparison of genetic structure with oceanographic model predictions allowed us to make predictions about the possible larval biology of the less well characterised urchin P. irregularis.

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Progress Code: completed
Maintenance and Update Frequency: notPlanned
Statement: Two urchin species (Heliocidaris erythrogramma and Phylacanthus irregularis) with ranges encompassing the lower west coast and southern coasts of Western Australia were collected by diving in coastal waters at four locations. Samples were either frozen for later processing in the laboratory or dissected in the field to extract gonads for placement into 95% ethanol. Genetic Testing: A total of 60 H. erythrogramma and 53 P. irregularis specimens were used in the genetic analyses. We obtained sequence from both mitochondrial (mtDNA) and nuclear DNA. For mtDNA analyses, a fragment of the mitochondrial cytochrome oxidase I subunit gene (COI) was amplified via the polymerase chain reaction (PCR) and sequenced using the LCO1490 and HCO2198 primers (Folmer et al., 1994). To obtain nuclear markers we targeted introns of seven single-copy genes, using universal coelomate primers. Sequencing was performed by Macrogen Ltd. (South Korea). Migrate: We used a maximum likelihood approach based on coalescent theory and implemented with the program Migrate-N 3.1.6 to estimate effective population size and migration among sampling sites. Population and search parameters were varied until quantitatively similar maximum likelihood values were returned from successive runs. Modelling: Lagrangian particle dispersal modelling was used to estimate larval connectivity potential among sites. This was nested within a data assimilated hydrodynamic model, BlueLINK with high spatial (0.1 degree) and temporal (6 hours) resolution reconstructing the hydrodynamic history around the Australian region between 1992 and 2006.

Notes

Credit
Western Australian Institute of Marine Science
Credit
Phillip England
Credit
Deryn Alpers
Credit
Asta Audzijonyte
Credit
Ming Feng
Credit
Dirk Slawinski
Credit
Nicole Murphy and Thomas Wernberg

Data time period: 2006-07-01 to 2011-06-30

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115.8,-30.2 115.8,-34 115,-34 115,-30.2 115.8,-30.2

115.4,-32.1

text: westlimit=115; southlimit=-34; eastlimit=115.8; northlimit=-30.2

Subjects
Coastal Waters (Australia) | West Australia Coast West, WA | Global / Oceans | East Indian Ocean | Global / Oceans | Indian Ocean | WAMSI Node 1 Project 2: Coastal Ecosystem Characterisation | Western Australian Marine Science Institute | biota | oceans |

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  • Local : Anzlic Identifier: ANZCW0306008949
  • Local : Marlin Record Number: 8949
  • global : bc1b3741-e336-5039-e044-00144f7bc0f4