Data

Vibrionaceae infection in phyllosomas of the tropical rock lobster, Panulirus ornatus

Name: Vibrionaceae infection in phyllosomas of the tropical rock lobster, Panulirus ornatus
Published: 2025

Australian Institute of Marine Science

Australian Institute of Marine Science (AIMS)

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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=https://apps.aims.gov.au/metadata/view/0367418b-d9d8-4111-8e28-1bc6c5a3f9ab&rft.title=Vibrionaceae infection in phyllosomas of the tropical rock lobster, Panulirus ornatus&rft.identifier=https://apps.aims.gov.au/metadata/view/0367418b-d9d8-4111-8e28-1bc6c5a3f9ab&rft.publisher=Australian Institute of Marine Science (AIMS)&rft.description=The total abundance of bacteria and relative abundance of Vibrionaceae cells associated with the larval stage of the tropical rock lobster, Panulirus ornatus, was investigated throughout a 22 day rearing trial using fluorescence in situ hybridisation.Approximately 20 live phyllosomas were randomly taken and removed from the tank on days 0, 2, 4, 7, 10, 14, 18 and 22 during a standard larval-rearing run and processed for FISH analysis. Bacterial abundance, the relative proportion of Vibrionaceae cells detected on external tissue and within the hepatopancreas were enumerated.Mean bacterial counts and the proportion of Vibrionaceae cells comprising the total bacteria were compared over time and analysed for increments of significance. To detect and enumerate total bacteria and Vibrionaceae associated with the larvae of the tropical rock lobster P. ornatus. The first study to provide in situ investigation elucidating the quantitative proliferation of potentially pathogenic bacteria associated with lobster phyllosoma during a larval rearing trial.The image is a photo of Vibrio infection in larval rock lobster tissue. The tissue has been dual hybridised with a vibrio-specific DNA probe (pink) and a bacteria-specific DNA probe (red). The image shows infection by both vibrio bacteria (pink cells) and other unidentified bacteria (red cells). The green is non specific fluorescence from the larval tissue.Maintenance and Update Frequency: notPlanned&rft.creator=Australian Institute of Marine Science (AIMS) &rft.date=2025&rft_rights=Creative Commons Attribution-NonCommercial 3.0 Australia License http://creativecommons.org/licenses/by-nc/3.0/au/&rft_rights=Use Limitation: All AIMS data, products and services are provided as is and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.&rft_rights=Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: Australian Institute of Marine Science (AIMS). (2009). Vibrionaceae infection in phyllosomas of the tropical rock lobster, Panulirus ornatus. https://apps.aims.gov.au/metadata/view/0367418b-d9d8-4111-8e28-1bc6c5a3f9ab, accessed[date-of-access].&rft_rights=Resource Usage:Use of the AIMS data is for not-for-profit applications only. All other users shall seek permission for use by contacting AIMS. Acknowledgements as prescribed must be clearly set out in the user's formal communications or publications.Access Constraint: intellectualPropertyRightsUse Constraint: intellectualPropertyRightsSecurity classification code: unclassifiedMetadata Usage:Access Constraint: intellectualPropertyRightsUse Constraint: intellectualPropertyRightsSecurity classification code: unclassified&rft_subject=oceans&rft.type=dataset&rft.language=English Access the data

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Creative Commons Attribution-NonCommercial 3.0 Australia License
http://creativecommons.org/licenses/by-nc/3.0/au/

Use Limitation: All AIMS data, products and services are provided "as is" and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.

Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: "Australian Institute of Marine Science (AIMS). (2009). Vibrionaceae infection in phyllosomas of the tropical rock lobster, Panulirus ornatus. https://apps.aims.gov.au/metadata/view/0367418b-d9d8-4111-8e28-1bc6c5a3f9ab, accessed[date-of-access]".

Resource Usage:Use of the AIMS data is for not-for-profit applications only. All other users shall seek permission for use by contacting AIMS. Acknowledgements as prescribed must be clearly set out in the user's formal communications or publications.Access Constraint: intellectualPropertyRightsUse Constraint: intellectualPropertyRightsSecurity classification code: unclassifiedMetadata Usage:Access Constraint: intellectualPropertyRightsUse Constraint: intellectualPropertyRightsSecurity classification code: unclassified

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The total abundance of bacteria and relative abundance of Vibrionaceae cells associated with the larval stage of the tropical rock lobster, Panulirus ornatus, was investigated throughout a 22 day rearing trial using fluorescence in situ hybridisation.Approximately 20 live phyllosomas were randomly taken and removed from the tank on days 0, 2, 4, 7, 10, 14, 18 and 22 during a standard larval-rearing run and processed for FISH analysis. Bacterial abundance, the relative proportion of Vibrionaceae cells detected on external tissue and within the hepatopancreas were enumerated.Mean bacterial counts and the proportion of Vibrionaceae cells comprising the total bacteria were compared over time and analysed for increments of significance.
To detect and enumerate total bacteria and Vibrionaceae associated with the larvae of the tropical rock lobster P. ornatus.
The first study to provide in situ investigation elucidating the quantitative proliferation of potentially pathogenic bacteria associated with lobster phyllosoma during a larval rearing trial.The image is a photo of Vibrio infection in larval rock lobster tissue. The tissue has been dual hybridised with a vibrio-specific DNA probe (pink) and a bacteria-specific DNA probe (red). The image shows infection by both vibrio bacteria (pink cells) and other unidentified bacteria (red cells). The green is non specific fluorescence from the larval tissue.

Lineage

Maintenance and Update Frequency: notPlanned

Notes

Credit
Webster, Nicole S, Dr (Principal Investigator)

Modified: 19 09 2025

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Other Information

Vibrionaceae infection in phyllosomas of the tropical rock lobster Panulirus ornatus as detected by fluorescence in situ hydridisation: Webster NS, Bourne DG and Hall MR (2006) Vibrionaceae infection in phyllosomas of the tropical rock lobster Panulirus ornatus as detected by fluorescence in situ hydridisation. Aquaculture 255: 173-178.

local : articleId=7158

Identifiers

global : 0367418b-d9d8-4111-8e28-1bc6c5a3f9ab