Data

UV-absorbing pigments in eye tissues of fish from the Great Barrier Reef

Name: UV-absorbing pigments in eye tissues of fish from the Great Barrier Reef
Published: 2026

Australian Institute of Marine Science

Australian Institute of Marine Science (AIMS)

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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=https://apps.aims.gov.au/metadata/view/012595de-6f4c-4e0a-b4e2-a6523f6f6809&rft.title=UV-absorbing pigments in eye tissues of fish from the Great Barrier Reef&rft.identifier=https://apps.aims.gov.au/metadata/view/012595de-6f4c-4e0a-b4e2-a6523f6f6809&rft.publisher=Australian Institute of Marine Science (AIMS)&rft.description=Ocular tissues from 52 species (19 families) of tropical marine fish were examined for levels of UV-absorbing pigments in the spectral region 300-380 nm.Specimens of mackerels, tuna and marlin were caught by surface trolling in Bowling Green and Upstart Bays; all other fish were caught by hand-line or spearfishing at Grub, Hunt and Marion Reefs. The eyes were removed and frozen immediately at -20°C. Lens ocular tissues were excised from defrosted samples, crushed and extracts formed by steeping in 5ml methanol. Visible-u.v. absorption spectra from these extracts were recorded. Solid protein biomass was determined from the solid tissue residues. Mycosporine-like amino acids (Asterina-330, palythene, palythine and palythinol) were separated and quantified (nmol/mg protein) using reverse-phase isocratic high-performance liquid chromatography (HPLC).The efficiency of methanol extraction This research was undertaken to:1. identify and describe the distribution of mycosporine-like amino acid compounds in the lens tissues of marine fish2. develop techniques for quantitative studies of UV absorbing compounds in fish eyes using high performance liquid chromotography3. evaluate the efficiency with which these compounds can be extracted from lens tissuesMaintenance and Update Frequency: notPlannedStatement: Statement: Visible UV absorption spectra of the methanol extracts were recorded using a Varian model 635 (field) and Hitachi model U-3200 (laboratory) scanning spectrophotometers.The HPLC method used to separate and quantify MAAs is described in:Dunlap WC and Chalker BE (1986) Identification and quantitation of near-u.v. absorbing compounds (S-320) in a hermatypic scleractinian. Coral Reefs 5: 155-159.&rft.creator=Australian Institute of Marine Science (AIMS) &rft.date=2026&rft.coverage=westlimit=147.20917; southlimit=-20.24283; eastlimit=152.38333; northlimit=-18.63333&rft.coverage=westlimit=147.20917; southlimit=-20.24283; eastlimit=152.38333; northlimit=-18.63333&rft_rights=Creative Commons Attribution-NonCommercial 3.0 Australia License http://creativecommons.org/licenses/by-nc/3.0/au/&rft_rights=Use Limitation: All AIMS data, products and services are provided as is and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.&rft_rights=Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: Australian Institute of Marine Science (AIMS). (2009). UV-absorbing pigments in eye tissues of fish from the Great Barrier Reef. https://apps.aims.gov.au/metadata/view/012595de-6f4c-4e0a-b4e2-a6523f6f6809, accessed[date-of-access].&rft_rights=Resource Usage:Use of the AIMS data is for not-for-profit applications only. All other users shall seek permission for use by contacting AIMS. Acknowledgements as prescribed must be clearly set out in the user's formal communications or publications.&rft_subject=oceans&rft.type=dataset&rft.language=English Access the data

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Creative Commons Attribution-NonCommercial 3.0 Australia License
http://creativecommons.org/licenses/by-nc/3.0/au/

Use Limitation: All AIMS data, products and services are provided "as is" and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.

Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: "Australian Institute of Marine Science (AIMS). (2009). UV-absorbing pigments in eye tissues of fish from the Great Barrier Reef. https://apps.aims.gov.au/metadata/view/012595de-6f4c-4e0a-b4e2-a6523f6f6809, accessed[date-of-access]".

Resource Usage:Use of the AIMS data is for not-for-profit applications only. All other users shall seek permission for use by contacting AIMS. Acknowledgements as prescribed must be clearly set out in the user's formal communications or publications.

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Ocular tissues from 52 species (19 families) of tropical marine fish were examined for levels of UV-absorbing pigments in the spectral region 300-380 nm.Specimens of mackerels, tuna and marlin were caught by surface trolling in Bowling Green and Upstart Bays; all other fish were caught by hand-line or spearfishing at Grub, Hunt and Marion Reefs. The eyes were removed and frozen immediately at -20°C. Lens ocular tissues were excised from defrosted samples, crushed and extracts formed by steeping in 5ml methanol. Visible-u.v. absorption spectra from these extracts were recorded. Solid protein biomass was determined from the solid tissue residues. Mycosporine-like amino acids (Asterina-330, palythene, palythine and palythinol) were separated and quantified (nmol/mg protein) using reverse-phase isocratic high-performance liquid chromatography (HPLC).The efficiency of methanol extraction
This research was undertaken to:1. identify and describe the distribution of mycosporine-like amino acid compounds in the lens tissues of marine fish2. develop techniques for quantitative studies of UV absorbing compounds in fish eyes using high performance liquid chromotography3. evaluate the efficiency with which these compounds can be extracted from lens tissues

Lineage

Maintenance and Update Frequency: notPlanned

Statement: Statement: Visible UV absorption spectra of the methanol extracts were recorded using a Varian model 635 (field) and Hitachi model U-3200 (laboratory) scanning spectrophotometers.The HPLC method used to separate and quantify MAAs is described in:Dunlap WC and Chalker BE (1986) Identification and quantitation of near-u.v. absorbing compounds (S-320) in a hermatypic scleractinian. Coral Reefs 5: 155-159.

Notes

Credit
Dunlap, Walter C, Dr (Principal Investigator)

Modified: 28 08 2026

This dataset is part of a larger collection

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text: westlimit=147.20917; southlimit=-20.24283; eastlimit=152.38333; northlimit=-18.63333

Subjects

oceans |

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Other Information

Biochemical photoadaptation in vision: UV-absorbing pigments in fish eye tissues: Dunlap WC, Williams DMcB, Chalker BE and Banaszak AT (1989) Biochemical photoadaptation in vision: UV-absorbing pigments in fish eye tissues. Comparative Biochemistry and Physiology 93B: 601-607.

local : articleId=2256

Identifiers

global : 012595de-6f4c-4e0a-b4e2-a6523f6f6809