Research Project
Full description Cereibacter sphaeroides is a versatile purple non-sulfur bacterium with potential applications in resource recovery, including the production of hydrogen, polyhydroxyalkanoates, single-cell protein, and other value-added products. In this study, a manually curated pathway-genome database was developed and is available on the BioCyc web portal. A genome-scale metabolic model representing the primary metabolism of C. sphaeroides ATCC 17025 was generated from the database, and flux balance analysis was performed. The predicted production rates of biomass, polyhydroxybutanoate, and hydrogen under anaerobic photoheterotrophic conditions were validated with experimental data. Using metabolic modeling, we investigated carbon flux distribution under various conditions and different organic substrates, gaining insights into the metabolic mechanisms driving the preferential generation of different products. This model can serve as a valuable tool for optimizing the production of value-added products.