Data

The behaviour of mixtures of paralytic shellfish toxins in receptor dependent assays

Australian Institute of Marine Science
Australian Institute of Marine Science (AIMS)
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=https://apps.aims.gov.au/metadata/view/7845898e-1944-4274-82ab-f48fd405e432&rft.title=The behaviour of mixtures of paralytic shellfish toxins in receptor dependent assays&rft.identifier=https://apps.aims.gov.au/metadata/view/7845898e-1944-4274-82ab-f48fd405e432&rft.publisher=Australian Institute of Marine Science (AIMS)&rft.description=Using two unrelated STX-binding proteins (the rat brain Na channel and a saxiphilin from the xanthid crab Liomera tristis), this study experimentally validated the predicted combined effects of binary and ternary mixtures of paralytic shellfish toxins (PSTs). All toxin dilutions (tritiated saxitoxin ([3H]STX), STX dihydrochloride, neoSTX, dcSTX, and GTX5) were in 0.01 M acetic acid. The L. tristis saxiphilin assay was conducted in high protein binding 96 well filtration plates and the rat brain Na channel binding of [3H]STX was measured using a microtiter plate method.The model predicts the amount of inhibition by toxin mixtures in competitive binding assays from which an IC50 (concentration that inhibits radioligand bindingby 50%) can be calculated. IC50 values and their 95% confidence limits from experimental data were derived using the single site curve equation of Graphpad Prism (Ver 4.0, Graphpad Software, San Diego, CA), with fitting constrained to a slope of 1 and a baseline of 0. To understand how toxin mixtures may act at the Na channel receptor via which PSTs exert their neurotoxicity.To test if the presence of weaker toxins dilutes stronger toxins The strong dominance of a mixture by the most potent toxins has implications for measurement of toxic test samples and for standards that may contain low levels of highly potent bioactive impurities.Maintenance and Update Frequency: notPlanned&rft.creator=Australian Institute of Marine Science (AIMS) &rft.date=2026&rft_rights=Creative Commons Attribution-NonCommercial 3.0 Australia License http://creativecommons.org/licenses/by-nc/3.0/au/&rft_rights=Use Limitation: All AIMS data, products and services are provided as is and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.&rft_rights=Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: Australian Institute of Marine Science (AIMS). (2009). The behaviour of mixtures of paralytic shellfish toxins in receptor dependent assays. https://apps.aims.gov.au/metadata/view/7845898e-1944-4274-82ab-f48fd405e432, accessed[date-of-access].&rft_rights=Resource Usage:Use of the AIMS data is for not-for-profit applications only. All other users shall seek permission for use by contacting AIMS. Acknowledgements as prescribed must be clearly set out in the user's formal communications or publications.&rft_subject=oceans&rft.type=dataset&rft.language=English Access the data
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Creative Commons Attribution-NonCommercial 3.0 Australia License
http://creativecommons.org/licenses/by-nc/3.0/au/

Use Limitation: All AIMS data, products and services are provided "as is" and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.

Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: "Australian Institute of Marine Science (AIMS). (2009). The behaviour of mixtures of paralytic shellfish toxins in receptor dependent assays. https://apps.aims.gov.au/metadata/view/7845898e-1944-4274-82ab-f48fd405e432, accessed[date-of-access]".

Resource Usage:Use of the AIMS data is for not-for-profit applications only. All other users shall seek permission for use by contacting AIMS. Acknowledgements as prescribed must be clearly set out in the user's formal communications or publications.

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Using two unrelated STX-binding proteins (the rat brain Na channel and a saxiphilin from the xanthid crab Liomera tristis), this study experimentally validated the predicted combined effects of binary and ternary mixtures of paralytic shellfish toxins (PSTs). All toxin dilutions (tritiated saxitoxin ([3H]STX), STX dihydrochloride, neoSTX, dcSTX, and GTX5) were in 0.01 M acetic acid. The L. tristis saxiphilin assay was conducted in high protein binding 96 well filtration plates and the rat brain Na channel binding of [3H]STX was measured using a microtiter plate method.The model predicts the amount of inhibition by toxin mixtures in competitive binding assays from which an IC50 (concentration that inhibits radioligand bindingby 50%) can be calculated. IC50 values and their 95% confidence limits from experimental data were derived using the single site curve equation of Graphpad Prism (Ver 4.0, Graphpad Software, San Diego, CA), with fitting constrained to a slope of 1 and a baseline of 0.
To understand how toxin mixtures may act at the Na channel receptor via which PSTs exert their neurotoxicity.To test if the presence of weaker toxins dilutes stronger toxins
The strong dominance of a mixture by the most potent toxins has implications for measurement of toxic test samples and for standards that may contain low levels of highly potent bioactive impurities.

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Maintenance and Update Frequency: notPlanned

Notes

Credit
Llewellyn, Lyndon E, Dr (Principal Investigator)

Modified: 18 09 2026

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The behaviour of mixtures of paralytic shellfish toxins in receptor dependent assays: Llewellyn LE (2006) The behaviour of mixtures of paralytic shellfish toxins in receptor dependent assays. Chemical Research in Toxicology 19:661-667.

local : articleId=7081

Background information on crab toxicity

url : http://www.aims.gov.au/pages/about/communications/backgrounders/20020905-killer-crabs.html

Identifiers
  • global : 7845898e-1944-4274-82ab-f48fd405e432
ACN 633 798 857