SWATH-MS Proteomic Profiling of Androgen and Anti-androgen Treated LNCaP and C42B Prostate Cancer Cell lines

ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.25912/RDF_1621567963490&rft.title=SWATH-MS Based Proteomic Profiling of Prostate Cancer Cells Reveals Adaptive Molecular Mechanisms in Response to Anti-Androgen Therapy&rft.identifier=10.25912/RDF_1621567963490&rft.publisher=Queensland University of Technology&rft.description=The current dataset was generated to explore the proteomic alterations stimulated in AR antagonist/anti-androgen (Bicalutamide and Enzalutamide) treated androgen-dependent cell model (LNCaP) in comparison with androgen-independent/castration-resistant cell model (C4-2B). Cells were seeded in 6-well plates using RPMI-1640+5% FBC and were incubated at 37 °C for 48 h. The medium was replaced with androgen-depleted culture medium containing 5% charcoal-striped serum for 48 h. Next, cells were supplemented with androgens: DHT (10 nM DHT), or anti-androgens: BICALUTAMIDE and ENZALUTAMIDE (10 μM) and Ethanol (EtOH: 20%) and incubated at 37 °C for additional 48 has described previously. C4-2B cells were cultured in RPMI-1640+5% FBS. The SWATH-MS label-free quantification method was used to obtain the total proteome of LNCaP and C42B cell lines. Current dataset provides the DDA and SWATH mass spectrometry files of androgen/anti-androgen treated LNCaP and C42B cell lines. &rft.creator=Liyanage, Chamikara &rft.creator=Malik, Mohammed Adil &rft.creator=Abeysinghe, Pevindu &rft.creator=Clements, Judith &rft.creator=Batra, Jyotsna &rft.date=2021&rft.edition=1&rft.relation=https://doi.org/10.3390/cancers13040715&rft.coverage=153.027190,-27.475887&rft_rights=© Queensland University of Technology, 2021. &rft_rights=Creative Commons Attribution 3.0 http://creativecommons.org/licenses/by/4.0/&rft_subject=Proteomics&rft_subject=Anti-androgen therapy&rft_subject=Bicalutamide&rft_subject=Transcriptomics&rft_subject=Prostate cancer&rft_subject=Enzalutamide&rft.type=dataset&rft.language=English Access the data