Data

Southern Ocean Chlorophyll-a, pigment and optical data from Aurora Australis AA 1997/98 V6

Australian Ocean Data Network
CSIRO O&A, Information & Data Centre (Point of contact) Clementson, Lesley (Point of contact) Data Officer (AR), Hobart (Processor of)
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=https://marlin.csiro.au/geonetwork/srv/eng/catalog.search#/metadata/68637f1a-de12-4740-a835-1b1273f5d311&rft.title=Southern Ocean Chlorophyll-a, pigment and optical data from Aurora Australis AA 1997/98 V6&rft.identifier=Anzlic Identifier: ANZCW0306006379&rft.publisher=Australian Ocean Data Network&rft.description=This record contains data collected at 15 stations on the Aurora Australis AA06/97 cruise, known as the SNARK cruise. Water samples were generally collected along a transect around 142degE from 42-55degS to determine the bio-optical characteristics of the region. Parameters measured are the concentration of chlorophyll and carotenoid pigments and the absorption coefficient for dissolved (CDOM) particulate (a/p) and detrital (a/d) components of the water column. In addition to the station samples 106 water samples were collected from the output of the underway fluorometer and analysed for chlorophyll and carotenoid pigments only. The BSI six-channel radiometer was deployed ot station.Maintenance and Update Frequency: notPlannedStatement: Water samples were taken on-board the Aurora Australis. Samples were filtered onboard. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. Pigment analysis 4 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters high performance liquid chromatograph, comprising a 600 controller, 717 plus refrigerated autosampler and a 996 photo-diode array detector. Pigments were separated using a stainless steel 25 cm x 4.6 mm I.D. column packed with ODS2 of 5 µm particle size (SGE) with gradient elution as described in Wright et al., [1991]. The separated pigments were detected at 436 nm and identified against standard spectra using Waters Millenium software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma) and all other pigment concentrations were determined from standards of purified pigments isolated from algal cultures. Spectral absorption. 2 litres of sample water was filtered through a 25 mm glass fibre filter (Whatman GF/F) and the filter then stored flat in liquid nitrogen until analysis. Optical density spectra for total particulate matter were obtained using a GBC 916 UV/VIS dual beam spectrophotometer equipped with an integrating sphere.&rft.creator=Anonymous&rft.date=2004&rft.coverage=westlimit=141; southlimit=-55; eastlimit=144; northlimit=-42&rft.coverage=westlimit=141; southlimit=-55; eastlimit=144; northlimit=-42&rft_rights=Release with the permission of the custodian.&rft_subject=oceans&rft_subject=PIGMENTS&rft_subject=EARTH SCIENCE&rft_subject=OCEANS&rft_subject=OCEAN CHEMISTRY&rft_subject=SUSPENDED SOLIDS&rft_subject=ABSORPTION&rft_subject=OCEAN OPTICS&rft_subject=FLUORESCENCE&rft_subject=GELBSTOFF&rft_subject=OCEAN COLOR&rft_subject=CHLOROPHYLL&rft_subject=TERRESTRIAL HYDROSPHERE&rft_subject=WATER QUALITY/WATER CHEMISTRY&rft_subject=CTDs (Conductivity-Temperature-Depth Profilers)&rft_subject=Fluorometers&rft_subject=HPLC (High Performance Liquid Chromatography)&rft_subject=Niskin Bottles&rft_subject=Spectrophotometers&rft_subject=Global / Oceans | Southern Ocean&rft_subject=WfO Biogeochemistry&rft_subject=Research Voyage: AU 1997/98 V6 SNARK&rft_subject=Biogeochemistry - CCRP 1998-2000&rft_subject=Ship: Aurora Australis&rft_subject=Practical salinity of the water body&rft_subject=Temperature of the water body&rft_subject=Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it&rft_subject=Pressure (measured variable) in the water body exerted by overlying sea water only&rft_subject=research vessel&rft_subject=CTD&rft.type=dataset&rft.language=English Access the data
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Brief description

This record contains data collected at 15 stations on the Aurora Australis AA06/97 cruise, known as the SNARK cruise. Water samples were generally collected along a transect around 142degE from 42-55degS to determine the bio-optical characteristics of the region. Parameters measured are the concentration of chlorophyll and carotenoid pigments and the absorption coefficient for dissolved (CDOM) particulate (a/p) and detrital (a/d) components of the water column. In addition to the station samples 106 water samples were collected from the output of the underway fluorometer and analysed for chlorophyll and carotenoid pigments only. The BSI six-channel radiometer was deployed ot station.

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Maintenance and Update Frequency: notPlanned
Statement: Water samples were taken on-board the Aurora Australis. Samples were filtered onboard. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. Pigment analysis 4 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters high performance liquid chromatograph, comprising a 600 controller, 717 plus refrigerated autosampler and a 996 photo-diode array detector. Pigments were separated using a stainless steel 25 cm x 4.6 mm I.D. column packed with ODS2 of 5 µm particle size (SGE) with gradient elution as described in Wright et al., [1991]. The separated pigments were detected at 436 nm and identified against standard spectra using Waters Millenium software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma) and all other pigment concentrations were determined from standards of purified pigments isolated from algal cultures. Spectral absorption. 2 litres of sample water was filtered through a 25 mm glass fibre filter (Whatman GF/F) and the filter then stored flat in liquid nitrogen until analysis. Optical density spectra for total particulate matter were obtained using a GBC 916 UV/VIS dual beam spectrophotometer equipped with an integrating sphere.

Notes

Credit
Lesley Clementson
Credit
Don McKenzie
Credit
Alison Turnbull

Data time period: 1998-03-03 to 1998-03-28

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144,-42 144,-55 141,-55 141,-42 144,-42

142.5,-48.5

text: westlimit=141; southlimit=-55; eastlimit=144; northlimit=-42

Subjects
Absorption | Biogeochemistry - CCRP 1998-2000 | Chlorophyll | CTD | CTDs (Conductivity-Temperature-Depth Profilers) | EARTH SCIENCE | Fluorescence | Fluorometers | Gelbstoff | Global / Oceans | Southern Ocean | HPLC (High Performance Liquid Chromatography) | Niskin Bottles | OCEAN CHEMISTRY | Ocean Color | OCEAN OPTICS | OCEANS | Pigments | Practical salinity of the water body | Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it | Pressure (measured variable) in the water body exerted by overlying sea water only | Research Voyage: AU 1997/98 V6 SNARK | Suspended Solids | Ship: Aurora Australis | Spectrophotometers | TERRESTRIAL HYDROSPHERE | Temperature of the water body | WATER QUALITY/WATER CHEMISTRY | WfO Biogeochemistry | oceans | research vessel |

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Access the data via the ARDC record (Data access)

uri : https://researchdata.edu.au/aurora-australis-voyage-underway-data/698582

Identifiers
  • global : 68637f1a-de12-4740-a835-1b1273f5d311
  • Local : Marlin Record Number: 6379
  • Local : Anzlic Identifier: ANZCW0306006379
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