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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.48610/3076584&rft.title=Sequences and scripts&rft.identifier=RDM ID: d691b270-4698-11ee-950c-2726e9e61885&rft.publisher=The University of Queensland&rft.description=# sequence-data folder The sequence-data folder contains all the metabarcoding data for this study and is organised in numbered folders. There is also a scripts folder here that contains the scripts used to generate the files in each step of the bioinformatics pipeline. See below a list of the sub-folders and a description of their contents... 1_raw (raw sequence data received from AGRF) 2_demux (demultiplexed files) 2_demux_trimmed (demultiplexed & trimmed reads) 3_merged (merged reads) 4_trimmomatic (quality filered reads) 5_trimmed (trimmed reads) 6_concat_not_unique (concantenated files) 7_concat_unique (concatenated files with duplicates removed) 8_derep (dereplicated reads) 9_denoised (reads following denoising steps) 10_length_filtered (reads post filtering by length) 11_chimeras_removed (chimeras removed) 12_mapped (tables resulting in the pipeline for use in taxonomic identification) 13_swarm (clustered reads into OTUs using swarm) 14_blast (echinoderm reads blasted against NCBI) 14_midori (all reads validated against MIDORI CO1 database) # community-data folder The community-data folder contains all the final files that were created at the end of the bioinformatics pipeline. The files were further curated and filtered in R and formatted for downstream analyses and figures.&rft.creator=Dr Iva Popovic&rft.creator=Dr Iva Popovic&rft.creator=Ms Ilha Byrne&rft.creator=Ms Ilha Byrne&rft.creator=Professor Cynthia Riginos&rft.creator=Professor Cynthia Riginos&rft.date=2023&rft_rights= http://guides.library.uq.edu.au/deposit_your_data/terms_and_conditions&rft_subject=eng&rft_subject=Population, Ecological and Evolutionary Genetics&rft_subject=BIOLOGICAL SCIENCES&rft_subject=GENETICS&rft.type=dataset&rft.language=English Access the data

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# sequence-data folder The sequence-data folder contains all the metabarcoding data for this study and is organised in numbered folders. There is also a scripts folder here that contains the scripts used to generate the files in each step of the bioinformatics pipeline. See below a list of the sub-folders and a description of their contents... 1_raw (raw sequence data received from AGRF) 2_demux (demultiplexed files) 2_demux_trimmed (demultiplexed & trimmed reads) 3_merged (merged reads) 4_trimmomatic (quality filered reads) 5_trimmed (trimmed reads) 6_concat_not_unique (concantenated files) 7_concat_unique (concatenated files with duplicates removed) 8_derep (dereplicated reads) 9_denoised (reads following denoising steps) 10_length_filtered (reads post filtering by length) 11_chimeras_removed (chimeras removed) 12_mapped (tables resulting in the pipeline for use in taxonomic identification) 13_swarm (clustered reads into OTUs using swarm) 14_blast (echinoderm reads blasted against NCBI) 14_midori (all reads validated against MIDORI CO1 database) # community-data folder The community-data folder contains all the final files that were created at the end of the bioinformatics pipeline. The files were further curated and filtered in R and formatted for downstream analyses and figures.

Issued: 31 08 2023

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