Data

Separation of dead and live fine mangrove roots in core samples from Hinchinbrook Island, north Queensland

Name: Separation of dead and live fine mangrove roots in core samples from Hinchinbrook Island, north Queensland
Published: 2025

Australian Institute of Marine Science

Australian Institute of Marine Science (AIMS)

Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]

ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=https://apps.aims.gov.au/metadata/view/5dcb4530-4ade-11dc-8f56-00008a07204e&rft.title=Separation of dead and live fine mangrove roots in core samples from Hinchinbrook Island, north Queensland&rft.identifier=https://apps.aims.gov.au/metadata/view/5dcb4530-4ade-11dc-8f56-00008a07204e&rft.publisher=Australian Institute of Marine Science (AIMS)&rft.description=The dead and live fine root content of mangrove soils was analysed from cores taken from Rhizophora stylosa and Ceriops tagal forests in Missionary Bay, Hinchinbrook Island.One metre long sediment cores, 6cm in diameter, were taken in mangrove forests in the high, mid and low inter-tidal zones. Cores were cut into 10 cm segments and sediment was removed from the roots by wet sieving in the field. Roots were stored in plastic bags at -18°C for up to 3 months until live and dead fine roots were separated using a technique involving solutions of colloidal silica (LUDOX). The method is based on separating roots on the basis that live roots have a lower specific gravity than dead roots. Optimal results were achieved when samples were first mixed in a 11% LUDOX solution. The top layer was then decanted off and further separated by mixing in a 6% LUDOX solution.The efficiency of the method was quantified by staining and examining all roots from separated samples microscopically, to identify dead and live roots not separated by the process. This research was undertaken to develop a rapid, highly efficient method for separating dead and live fine roots to enable investigation of fine root mass and the role of fine root dynamics in plant community materials budgets in tropical mangrove forests.Maintenance and Update Frequency: notPlannedStatement: Statement: The separation method was based on a method developed for separating dead plant material from zooplankton in mangrove forest water samples, which is described in:Robertson AI, Dixon P, Daniel PA (1988) Zooplankton dynamics in mangrove and other nearshore habitats in tropical Australia. Mar. Ecol. Progr. Ser. 43, 139-150.&rft.creator=Australian Institute of Marine Science (AIMS) &rft.date=2025&rft.coverage=westlimit=146.0; southlimit=-18.5; eastlimit=146.5; northlimit=-18.0&rft.coverage=westlimit=146.0; southlimit=-18.5; eastlimit=146.5; northlimit=-18.0&rft_rights=Creative Commons Attribution-NonCommercial 3.0 Australia License http://creativecommons.org/licenses/by-nc/3.0/au/&rft_rights=Use Limitation: All AIMS data, products and services are provided as is and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.&rft_rights=Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: Australian Institute of Marine Science (AIMS). (2009). Separation of dead and live fine mangrove roots in core samples from Hinchinbrook Island, north Queensland. https://apps.aims.gov.au/metadata/view/5dcb4530-4ade-11dc-8f56-00008a07204e, accessed[date-of-access].&rft_rights=Resource Usage:Use of the AIMS data is for not-for-profit applications only. All other users shall seek permission for use by contacting AIMS. Acknowledgements as prescribed must be clearly set out in the user's formal communications or publications.&rft_subject=oceans&rft.type=dataset&rft.language=English Access the data

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Creative Commons Attribution-NonCommercial 3.0 Australia License
http://creativecommons.org/licenses/by-nc/3.0/au/

Use Limitation: All AIMS data, products and services are provided "as is" and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.

Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: "Australian Institute of Marine Science (AIMS). (2009). Separation of dead and live fine mangrove roots in core samples from Hinchinbrook Island, north Queensland. https://apps.aims.gov.au/metadata/view/5dcb4530-4ade-11dc-8f56-00008a07204e, accessed[date-of-access]".

Resource Usage:Use of the AIMS data is for not-for-profit applications only. All other users shall seek permission for use by contacting AIMS. Acknowledgements as prescribed must be clearly set out in the user's formal communications or publications.

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The dead and live fine root content of mangrove soils was analysed from cores taken from Rhizophora stylosa and Ceriops tagal forests in Missionary Bay, Hinchinbrook Island.One metre long sediment cores, 6cm in diameter, were taken in mangrove forests in the high, mid and low inter-tidal zones. Cores were cut into 10 cm segments and sediment was removed from the roots by wet sieving in the field. Roots were stored in plastic bags at -18°C for up to 3 months until live and dead fine roots were separated using a technique involving solutions of colloidal silica (LUDOX). The method is based on separating roots on the basis that live roots have a lower specific gravity than dead roots. Optimal results were achieved when samples were first mixed in a 11% LUDOX solution. The top layer was then decanted off and further separated by mixing in a 6% LUDOX solution.The efficiency of the method was quantified by staining and examining all roots from separated samples microscopically, to identify dead and live roots not separated by the process.
This research was undertaken to develop a rapid, highly efficient method for separating dead and live fine roots to enable investigation of fine root mass and the role of fine root dynamics in plant community materials budgets in tropical mangrove forests.

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Maintenance and Update Frequency: notPlanned

Statement: Statement: The separation method was based on a method developed for separating dead plant material from zooplankton in mangrove forest water samples, which is described in:Robertson AI, Dixon P, Daniel PA (1988) Zooplankton dynamics in mangrove and other nearshore habitats in tropical Australia. Mar. Ecol. Progr. Ser. 43, 139-150.

Notes

Credit
Robertson, Alistar I, Dr (Principal Investigator)

Modified: 19 09 2025

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text: westlimit=146.0; southlimit=-18.5; eastlimit=146.5; northlimit=-18.0

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oceans |

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Other Information

Separating live and dead fine roots using colloidal silica: an example from mangrove forests: Robertson AI and Dixon P (1993) Separating live and dead fine roots using colloidal silica: an example from mangrove forests. Plant and Soil 157: 151-154.

local : articleId=2485

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global : 5dcb4530-4ade-11dc-8f56-00008a07204e