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Sensitive environmental DNA detection via lateral flow assay (dipstick) - A case study on corallivorous crown-of-thorns sea star (Acanthaster cf. solaris) detection

Australian Institute of Marine Science
Australian Institute of Marine Science (AIMS)
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=https://apps.aims.gov.au/metadata/view/e19d6487-3283-46e3-b0da-1185b66d9e97&rft.title=Sensitive environmental DNA detection via lateral flow assay (dipstick) - A case study on corallivorous crown-of-thorns sea star (Acanthaster cf. solaris) detection&rft.identifier=https://apps.aims.gov.au/metadata/view/e19d6487-3283-46e3-b0da-1185b66d9e97&rft.publisher=Australian Institute of Marine Science (AIMS)&rft.description=Detection of low-density CoTS populations is critical for early warning of outbreaks and can lead to early management interventions (e.g., culling). A lateral flow assay method for eDNA detection was developed using a commercially available nucleic acid lateral flow device (PCRD™) in combination with a previously developed species-specific mtDNA primer. This method was applied to eDNA water samples collected in field locations where CoTS were present in low (=nonoutbreak) population densities to demonstrate the sensitivity of the method to detect these populations.Aquarium raised 6- to 12-month-old juvenile CoTS were used for laboratory testing to prepare dilution series of genomic DNA.A total of 58 water samples were collected from two locations on the Great Barrier Reef (GBR), Australia; 40 samples from Big Vicky's Reef in August 2019 and 18 samples from Elizabeth Reef in May 2019. See Doyle and Uthicke (2021) for full sample processing procedures.Each field sample was analysed via ddPCR and PCR/LFA and assay sensitivity was compared as well as an assessment of the cost and time to complete sample analysis.All data associated with this study can be found either in the main text or appendices of Doyle and Uthicke (2021) linked below.Maintenance and Update Frequency: asNeeded&rft.creator=Australian Institute of Marine Science (AIMS) &rft.date=2025&rft.coverage=westlimit=149.05045; southlimit=-19.334333; eastlimit=149.05045; northlimit=-19.334333&rft.coverage=westlimit=149.05045; southlimit=-19.334333; eastlimit=149.05045; northlimit=-19.334333&rft.coverage=westlimit=145.43935; southlimit=-14.683833; eastlimit=145.43935; northlimit=-14.683833&rft.coverage=westlimit=145.43935; southlimit=-14.683833; eastlimit=145.43935; northlimit=-14.683833&rft_rights=Creative Commons Attribution 3.0 Australia License http://creativecommons.org/licenses/by/3.0/au/&rft_rights=Use Limitation: All AIMS data, products and services are provided as is and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.&rft_rights=Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: Australian Institute of Marine Science (AIMS). (2021). Sensitive environmental DNA detection via lateral flow assay (dipstick) - A case study on corallivorous crown-of-thorns sea star (Acanthaster cf. solaris) detection. https://apps.aims.gov.au/metadata/view/e19d6487-3283-46e3-b0da-1185b66d9e97, accessed[date-of-access].&rft_subject=oceans&rft_subject=Abundance of biota&rft.type=dataset&rft.language=English Access the data
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Creative Commons Attribution 3.0 Australia License
http://creativecommons.org/licenses/by/3.0/au/

Use Limitation: All AIMS data, products and services are provided "as is" and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.

Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: "Australian Institute of Marine Science (AIMS). (2021). Sensitive environmental DNA detection via lateral flow assay (dipstick) - A case study on corallivorous crown-of-thorns sea star (Acanthaster cf. solaris) detection. https://apps.aims.gov.au/metadata/view/e19d6487-3283-46e3-b0da-1185b66d9e97, accessed[date-of-access]".

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Detection of low-density CoTS populations is critical for early warning of outbreaks and can lead to early management interventions (e.g., culling). A lateral flow assay method for eDNA detection was developed using a commercially available nucleic acid lateral flow device (PCRD™) in combination with a previously developed species-specific mtDNA primer. This method was applied to eDNA water samples collected in field locations where CoTS were present in low (=nonoutbreak) population densities to demonstrate the sensitivity of the method to detect these populations.


Aquarium raised 6- to 12-month-old juvenile CoTS were used for laboratory testing to prepare dilution series of genomic DNA.


A total of 58 water samples were collected from two locations on the Great Barrier Reef (GBR), Australia; 40 samples from Big Vicky's Reef in August 2019 and 18 samples from Elizabeth Reef in May 2019. See Doyle and Uthicke (2021) for full sample processing procedures.


Each field sample was analysed via ddPCR and PCR/LFA and assay sensitivity was compared as well as an assessment of the cost and time to complete sample analysis.


All data associated with this study can be found either in the main text or appendices of Doyle and Uthicke (2021) linked below.

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Maintenance and Update Frequency: asNeeded

Notes

Credit
Uthicke, S. (AIMS)
Credit
Doyle, J. Australian Institute of Marine Science (AIMS)

Modified: 18 07 2025

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149.05045,-19.33433

149.05045,-19.334333

145.43935,-14.68383

145.43935,-14.683833

text: westlimit=149.05045; southlimit=-19.334333; eastlimit=149.05045; northlimit=-19.334333

text: westlimit=145.43935; southlimit=-14.683833; eastlimit=145.43935; northlimit=-14.683833

Subjects
Abundance of biota | oceans |

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Other Information
Doyle, J, Uthicke, S. Sensitive environmental DNA detection via lateral flow assay (dipstick)—A case study on corallivorous crown-of-thorns sea star (Acanthaster cf. solaris) detection. Environmental DNA. 2021; 3: 323– 342. https://doi.org/10.1002/edn3.123

doi : https://doi.org/10.1002/edn3.123

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  • global : e19d6487-3283-46e3-b0da-1185b66d9e97