Data

RNA sequence data from krill CO2 exposure experiment

Australian Ocean Data Network
Deagle, B. ; DEAGLE, BRUCE
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=http://data.aad.gov.au/metadata/records/AAS_4015_krill_RNA&rft.title=RNA sequence data from krill CO2 exposure experiment&rft.identifier=http://data.aad.gov.au/metadata/records/AAS_4015_krill_RNA&rft.publisher=Australian Antarctic Data Centre&rft.description=We use RNA sequencing to investigate which genetic/physiological pathways in Antarctic krill are affected by increased CO2 levels. We carried out larval CO2 exposure experiments in March 2012 at the AAD aquarium. Two developmental stages were used (Calyptopis I and Furcilia V) and three CO2 levels (control, 1000 and 2000 ppm). These were short term experiments (2 days) - since initial longer experiments starting with fertilized eggs resulted in differences in developmental stages between treatments and control which could confound the data. RNA was extracted from larvae and high-throughput RNA sequencing (RNA-seq) was carried out on 6 samples (2 stages * 3 treatments). Sequencing was carried out on an Illumina sequencer (Genome Analyzer II). We collected ~ 60 million sequence reads per sample (Data in FASTA format each read gives 100 base pairs of sequence), so a total of ~360 million reads (36 billion bp of data).Progress Code: completed&rft.creator=Deagle, B. &rft.creator=DEAGLE, BRUCE &rft.date=2016&rft.coverage=westlimit=147.29014; southlimit=-42.98792; eastlimit=147.29396; northlimit=-42.98550&rft.coverage=westlimit=147.29014; southlimit=-42.98792; eastlimit=147.29396; northlimit=-42.98550&rft_rights=This metadata record is publicly available.&rft_rights=These data are publicly available for download. However, owing to their size, they will be placed on an FTP server at a user's request.&rft_rights= https://creativecommons.org/licenses/by/4.0/legalcode&rft_rights=This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4015_krill_RNA when using these data. http://creativecommons.org/licenses/by/4.0/).&rft_rights=Portable Network Graphic&rft_rights=https://i.creativecommons.org/l/by/3.0/88x31.png&rft_rights=Creative Commons by Attribution logo&rft_rights=Attribution 4.0 International (CC BY 4.0)&rft_rights=Legal code for Creative Commons by Attribution 4.0 International license&rft_rights=Attribution 4.0 International (CC BY 4.0)&rft_rights= https://creativecommons.org/licenses/by/4.0/legalcode&rft_subject=biota&rft_subject=oceans&rft_subject=environment&rft_subject=EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/INVERTEBRATES > ARTHROPODS > CRUSTACEANS > EUPHAUSIIDS (KRILL)&rft_subject=EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > CARBON DIOXIDE&rft_subject=OCEAN ACIDIFICATION&rft_subject=RNA&rft_subject=GENETICS&rft_subject=ADS > Automated DNA Sequencer&rft_subject=LABORATORY&rft_subject=AMD/AU&rft_subject=AMD&rft_subject=CEOS&rft_subject=GEOGRAPHIC REGION > POLAR&rft_subject=CONTINENT > ANTARCTICA&rft_subject=OCEAN > SOUTHERN OCEAN&rft_subject=CONTINENT > AUSTRALIA/NEW ZEALAND > AUSTRALIA&rft.type=dataset&rft.language=English Access the data
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This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/).

Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4015_krill_RNA when using these data.
http://creativecommons.org/licenses/by/4.0/).

Attribution 4.0 International (CC BY 4.0)

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This metadata record is publicly available.

These data are publicly available for download. However, owing to their size, they will be placed on an FTP server at a user's request.

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Brief description

We use RNA sequencing to investigate which genetic/physiological pathways in Antarctic krill are affected by increased CO2 levels. We carried out larval CO2 exposure experiments in March 2012 at the AAD aquarium. Two developmental stages were used (Calyptopis I and Furcilia V) and three CO2 levels (control, 1000 and 2000 ppm). These were short term experiments (2 days) - since initial longer experiments starting with fertilized eggs resulted in differences in developmental stages between treatments and control which could confound the data. RNA was extracted from larvae and high-throughput RNA sequencing (RNA-seq) was carried out on 6 samples (2 stages * 3 treatments). Sequencing was carried out on an Illumina sequencer (Genome Analyzer II). We collected ~ 60 million sequence reads per sample (Data in FASTA format each read gives 100 base pairs of sequence), so a total of ~360 million reads (36 billion bp of data).

Lineage

Progress Code: completed

Data time period: 2012-03-01 to 2012-09-30

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147.29396,-42.9855 147.29396,-42.98792 147.29014,-42.98792 147.29014,-42.9855 147.29396,-42.9855

147.29205,-42.98671

text: westlimit=147.29014; southlimit=-42.98792; eastlimit=147.29396; northlimit=-42.98550

Subjects
ADS > Automated DNA Sequencer | AMD | AMD/AU | CEOS | CONTINENT > ANTARCTICA | CONTINENT > AUSTRALIA/NEW ZEALAND > AUSTRALIA | EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/INVERTEBRATES > ARTHROPODS > CRUSTACEANS > EUPHAUSIIDS (KRILL) | EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > CARBON DIOXIDE | GENETICS | GEOGRAPHIC REGION > POLAR | LABORATORY | OCEAN > SOUTHERN OCEAN | OCEAN ACIDIFICATION | RNA | biota | environment | oceans |

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Identifiers
  • global : AAS_4015_krill_RNA
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