Data

Reciprocal transplant experiments of mutagen treated Symbiodiniaceae to ambient and elevated temperatures

Australian Institute of Marine Science
Australian Institute of Marine Science (AIMS)
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=https://apps.aims.gov.au/metadata/view/a86b9bca-1982-4669-ab81-a1c6fb8c583e&rft.title=Reciprocal transplant experiments of mutagen treated Symbiodiniaceae to ambient and elevated temperatures&rft.identifier=https://apps.aims.gov.au/metadata/view/a86b9bca-1982-4669-ab81-a1c6fb8c583e&rft.publisher=Australian Institute of Marine Science (AIMS)&rft.description=We investigated the adaptive responses of coral photosymbionts (Symbiodiniaceae) following chemical mutagenesis and long-term thermal selection.Strains of Durusdinium trenchii, Fugacium kawagutii and Symbiodinium pilosum were exposed to ethyl methanesulfonate to induce random mutagenesis, and then underwent thermal selection at 31/33°C. After 4.6-5 years of experimental evolution, the in vitro thermal tolerance of these strains was assessed via reciprocal transplant experiments to ambient (27°C) and elevated (31/35°C) temperatures. We measured the growth, photochemical efficiency, oxidative stress and nutrient use of these heat-evolved strains and wild-type strains under both temperature treatments.This dataset contains data collected during the reciprocal transplant experiments. All the measurements were carried out at the Symbiont Culture Facility at AIMS between 01.10.2021 and 01.05.2022.Maintenance and Update Frequency: notPlannedStatement: Cell density data was collected using a flow cytometer (BD Accuri™ C6 Plus Flow Cytometer, BD Biosciences). Photochemical efficiency data (Fv/Fm and Qm) was collected using an imaging pulse-amplitude modulation chlorophyll fluorometer (Maxi version IMAGING PAM M-Series, Walz). Reactive oxgen species levels were assayed by staining Symbiodiniaceae cells and culture medium with CellROX Green or CellROX Orange Reagents (Thermo Fisher Scientific), respectively. The stained samples were then processed using a flow cytometer (BD FACSVerse, BD Biosciences) and a microplate reader (Synergy H4, BioTek). Nutrient concentrations were determined by the Analytical Technology Laboratory at AIMS by processing Symbiodiniaceae culture medium with a segmented flow analyzer (AA3 HR AutoAnalyzer, Seal Analytical).&rft.creator=Australian Institute of Marine Science (AIMS) &rft.date=2026&rft.coverage=westlimit=147.0553278923035; southlimit=-19.266707963412053; eastlimit=147.0553278923035; northlimit=-19.266707963412053&rft.coverage=westlimit=147.0553278923035; southlimit=-19.266707963412053; eastlimit=147.0553278923035; northlimit=-19.266707963412053&rft_rights=Creative Commons Attribution 3.0 Australia License http://creativecommons.org/licenses/by/3.0/au/&rft_rights=Use Limitation: All AIMS data, products and services are provided as is and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.&rft_rights=Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: Australian Institute of Marine Science (AIMS). (2022). Reciprocal transplant experiments of mutagen treated Symbiodiniaceae to ambient and elevated temperatures. https://apps.aims.gov.au/metadata/view/a86b9bca-1982-4669-ab81-a1c6fb8c583e, accessed[date-of-access].&rft_subject=oceans&rft.type=dataset&rft.language=English Access the data
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Creative Commons Attribution 3.0 Australia License
http://creativecommons.org/licenses/by/3.0/au/

Use Limitation: All AIMS data, products and services are provided "as is" and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.

Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: "Australian Institute of Marine Science (AIMS). (2022). Reciprocal transplant experiments of mutagen treated Symbiodiniaceae to ambient and elevated temperatures. https://apps.aims.gov.au/metadata/view/a86b9bca-1982-4669-ab81-a1c6fb8c583e, accessed[date-of-access]".

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We investigated the adaptive responses of coral photosymbionts (Symbiodiniaceae) following chemical mutagenesis and long-term thermal selection.


Strains of Durusdinium trenchii, Fugacium kawagutii and Symbiodinium pilosum were exposed to ethyl methanesulfonate to induce random mutagenesis, and then underwent thermal selection at 31/33°C. After 4.6-5 years of experimental evolution, the in vitro thermal tolerance of these strains was assessed via reciprocal transplant experiments to ambient (27°C) and elevated (31/35°C) temperatures. We measured the growth, photochemical efficiency, oxidative stress and nutrient use of these heat-evolved strains and wild-type strains under both temperature treatments.


This dataset contains data collected during the reciprocal transplant experiments. All the measurements were carried out at the Symbiont Culture Facility at AIMS between 01.10.2021 and 01.05.2022.

Lineage

Maintenance and Update Frequency: notPlanned
Statement: Cell density data was collected using a flow cytometer (BD Accuri™ C6 Plus Flow Cytometer, BD Biosciences). Photochemical efficiency data (Fv/Fm and Qm) was collected using an imaging pulse-amplitude modulation chlorophyll fluorometer (Maxi version IMAGING PAM M-Series, Walz). Reactive oxgen species levels were assayed by staining Symbiodiniaceae cells and culture medium with CellROX Green or CellROX Orange Reagents (Thermo Fisher Scientific), respectively. The stained samples were then processed using a flow cytometer (BD FACSVerse, BD Biosciences) and a microplate reader (Synergy H4, BioTek). Nutrient concentrations were determined by the Analytical Technology Laboratory at AIMS by processing Symbiodiniaceae culture medium with a segmented flow analyzer (AA3 HR AutoAnalyzer, Seal Analytical).

Notes

Credit
Chan, WY. The University of Melbourne
Credit
Scharfenstein, HJ. Australian Institute of Marine Science (AIMS) & The University of Melbourne
Credit
Peplow, LM (AIMS)
Credit
Paul G. Allen Family Foundation, USA
Credit
Reef Restoration and Adaptation Program, Australia
Credit
Alvarez-Roa, C. AIMS
Credit
van Oppen, MJH. AIMS & The University of Melbourne
Credit
ARC Laureate Fellowship, No. FL180100036, Australian Research Council, Australia
Credit
Buerger, P. Macquarie University

Modified: 18 09 2026

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147.05533,-19.26671

147.0553278923,-19.266707963412

text: westlimit=147.0553278923035; southlimit=-19.266707963412053; eastlimit=147.0553278923035; northlimit=-19.266707963412053

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Other Information
Scharfenstein, H. J., Alvarez-Roa, C., Peplow, L. M., Buerger, P., Chan, W. Y., & van Oppen, M. J. H. (2023). Chemical mutagenesis and thermal selection of coral photosymbionts induce adaptation to heat stress with trait trade-offs. Evolutionary Applications, 16, 1549–1567. https://doi.org/10.1111/eva.13586

doi : https://doi.org/10.1111/eva.13586

GitHub Repository: https://github.com/hscharfenstein/Chemical-mutagenesis-and-thermal-selection-of-Symbiodiniaceae

url : https://github.com/hscharfenstein/Chemical-mutagenesis-and-thermal-selection-of-Symbiodiniaceae

Physiological data from RTEs

url : https://api.aims.gov.au/data-v2.0/a86b9bca-1982-4669-ab81-a1c6fb8c583e/files/RTE data.xlsx

Identifiers
  • global : a86b9bca-1982-4669-ab81-a1c6fb8c583e
ACN 633 798 857