Data

Proteome data of the raw and the heated Pacific oyster (Crassostrea gigas)

James Cook University
Lopata, Andreas ; Kamath, Sandip ; Nugraha, Roni
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.4225/28/588eb674aa910&rft.title=Proteome data of the raw and the heated Pacific oyster (Crassostrea gigas)&rft.identifier=10.4225/28/588eb674aa910&rft.publisher=James Cook University&rft.description=Protein compositions of the Pacific oyster extracts were identified through bottom-up mass spectrometry approach. The proteins were separated on an SDS-PAGE gel, cut into small pieces and tryptic digested (Fig 3A). The peptide mixtures were then subjected to LC-MS/MS analysis generating a total of 81831 spectra for the raw extract and 88130 spectra for the heated extract. Mascot searches were conducted against Pacific oyster proteomes, and the identified proteins were validated by Scaffold (version Scaffold_4.7.3) using criteria at least two peptides at a 99.0% probability. In total Scaffold identified 1,086 proteins from raw extract and 130 proteins from the heated extract, with 103 proteins are shared between the two extracts. These datasets contain proteins identified by mass spectrometry in the raw and the heated sample of Pacific oyster (C. gigas)&rft.creator=Lopata, Andreas &rft.creator=Kamath, Sandip &rft.creator=Nugraha, Roni &rft.date=2017&rft.relation=http://dx.doi.org/10.1111/imj.16_13197&rft_rights=Once access to the data has been obtained via negotiation with the data manager, use of the dataset is governed by the CC-BY-NC AU licence.&rft_rights=CC BY-NC: Attribution-Noncommercial 3.0 AU http://creativecommons.org/licenses/by-nc/3.0/au&rft_subject=Pacific oyster&rft_subject=proteome&rft_subject=mass spectrometry&rft.type=dataset&rft.language=English Access the data
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CC-BY-NC

CC BY-NC: Attribution-Noncommercial 3.0 AU
http://creativecommons.org/licenses/by-nc/3.0/au

Once access to the data has been obtained via negotiation with the data manager, use of the dataset is governed by the CC-BY-NC AU licence.

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Conditional: Contact researchdata@jcu.edu.au to request access to this data.

Brief description

These datasets contain proteins identified by mass spectrometry in the raw and the heated sample of Pacific oyster (C. gigas)

Full description

Protein compositions of the Pacific oyster extracts were identified through bottom-up mass spectrometry approach. The proteins were separated on an SDS-PAGE gel, cut into small pieces and tryptic digested (Fig 3A). The peptide mixtures were then subjected to LC-MS/MS analysis generating a total of 81831 spectra for the raw extract and 88130 spectra for the heated extract. Mascot searches were conducted against Pacific oyster proteomes, and the identified proteins were validated by Scaffold (version Scaffold_4.7.3) using criteria at least two peptides at a 99.0% probability. In total Scaffold identified 1,086 proteins from raw extract and 130 proteins from the heated extract, with 103 proteins are shared between the two extracts.

Notes

This dataset consists of a spreadsheet in MS Excel (.xlsx) and Open Document formats (.ods)

Created: 2017-01-30

Data time period: 23 08 2017

This dataset is part of a larger collection

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Subjects
Pacific oyster | mass spectrometry | proteome |

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Identifiers
  • DOI : 10.4225/28/588EB674AA910
  • Local : researchdata.jcu.edu.au//published/8a0dc4110b405eac8e6e7be6400f1576
  • Local : 760de7722ad0ffe4c05ded6986681fb5
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