Data

Pre-emergent phytotoxicity using image analysis

Australian Institute of Marine Science
Australian Institute of Marine Science (AIMS)
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=https://apps.aims.gov.au/metadata/view/6a40fbfa-5390-481e-99ac-1861ea8d1330&rft.title=Pre-emergent phytotoxicity using image analysis&rft.identifier=https://apps.aims.gov.au/metadata/view/6a40fbfa-5390-481e-99ac-1861ea8d1330&rft.publisher=Australian Institute of Marine Science (AIMS)&rft.description=A simple quantitative bioassay was developed using widely available technology to determine pre-emergent phytotoxicity. Digitaria didactyla (Queensland Blue Couch Grass) was assessed by analysing digital images of the plants growing in microtitre plates for the effects of herbicides on germination. The herbicides tested were metsulfuron, triasulfuron, fluometuron atrazine, simazine and prometryn. Images of each 24-well microtitre plate (taken with a digital camera 7-10 days after planting), were analysed using a public domain image processing program, and a filter (developed using plugins available within the program) selected pixels within the green spectrum. Pixel counting took place using a spreadsheet function. To investigate the use of microtitre plates for the discovery of phytotoxic compounds within higher plants. This bioassay system allows rapid throughput of test samples with minimum handling.Maintenance and Update Frequency: notPlanned&rft.creator=Australian Institute of Marine Science (AIMS) &rft.date=2025&rft_rights=All AIMS data, products and services are provided as is and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.&rft_rights=The data was collected under contract between AIMS and another party(s). Specific agreements for access and use of the data shall be negotiated separately. Contact the AIMS Data Centre ([email protected]) for further information&rft_rights=Resource Usage:Indemnity: Whilst every care has been taken to ensure the data are of high quality, no guarantees are provided on the accuracy of the data and no liability is accepted for any loss, damage or injury resulting from the use of these data.Access Constraint: restrictedUse Constraint: intellectualPropertyRightsSecurity classification code: unclassifiedMetadata Usage:Access Constraint: intellectualPropertyRightsUse Constraint: intellectualPropertyRightsSecurity classification code: unclassified&rft_subject=oceans&rft.type=dataset&rft.language=English Access the data
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All AIMS data, products and services are provided "as is" and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.

The data was collected under contract between AIMS and another party(s). Specific agreements for access and use of the data shall be negotiated separately. Contact the AIMS Data Centre ([email protected]) for further information

Resource Usage:Indemnity: Whilst every care has been taken to ensure the data are of high quality, no guarantees are provided on the accuracy of the data and no liability is accepted for any loss, damage or injury resulting from the use of these data.Access Constraint: restrictedUse Constraint: intellectualPropertyRightsSecurity classification code: unclassifiedMetadata Usage:Access Constraint: intellectualPropertyRightsUse Constraint: intellectualPropertyRightsSecurity classification code: unclassified

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A simple quantitative bioassay was developed using widely available technology to determine pre-emergent phytotoxicity. Digitaria didactyla (Queensland Blue Couch Grass) was assessed by analysing digital images of the plants growing in microtitre plates for the effects of herbicides on germination. The herbicides tested were metsulfuron, triasulfuron, fluometuron atrazine, simazine and prometryn. Images of each 24-well microtitre plate (taken with a digital camera 7-10 days after planting), were analysed using a public domain image processing program, and a filter (developed using plugins available within the program) selected pixels within the green spectrum. Pixel counting took place using a spreadsheet function.
To investigate the use of microtitre plates for the discovery of phytotoxic compounds within higher plants.
This bioassay system allows rapid throughput of test samples with minimum handling.

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Maintenance and Update Frequency: notPlanned

Notes

Credit
Doyle, Jason R, Mr (Principal Investigator)

Modified: 19 09 2025

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  • global : 6a40fbfa-5390-481e-99ac-1861ea8d1330