Data

Physiological diversity among sympatric, conspecific endosymbionts of coral (Cladocopium C1acro) from the Great Barrier Reef

Name: Physiological diversity among sympatric, conspecific endosymbionts of coral (Cladocopium C1acro) from the Great Barrier Reef
Published: 2026

Australian Institute of Marine Science

Australian Institute of Marine Science (AIMS)

Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]

ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=https://apps.aims.gov.au/metadata/view/84609d81-15a3-47f5-9bb4-60a6d9557dd4&rft.title=Physiological diversity among sympatric, conspecific endosymbionts of coral (Cladocopium C1acro) from the Great Barrier Reef&rft.identifier=https://apps.aims.gov.au/metadata/view/84609d81-15a3-47f5-9bb4-60a6d9557dd4&rft.publisher=Australian Institute of Marine Science (AIMS)&rft.description=Strains of the common coral endosymbiont, Cladocopium C1acro were isolated from four Acropora corals collected from inshore sites on the Great Barrier Reef (Nelly Bay, Magnetic Island, and South Molle Island in the Whitsunday Islands). Colonies of Accropora Tenuis and Acropora millepora were growing at similar depths and photic habitats, between `~3-4m.Before the culturing of the symbionts, freshly isolated cells from the host were genotyped. Clonal cultures were established using methods described in Beltrán, et.al. (2021).For the generation of clonal cultures, two methods were used. Three of the four clonal cultures (Aten-MI-1, Aten-WSY, and Amil-MI) were obtained after plating the heterogeneous population onto IMK − 1% agar plus antibiotics and incubating them under the conditions as above and picking a single colony-forming unit. The fourth clonal culture (Aten-MI-2) was produced from a single cell isolated using a micro-manipulator (MN-153, Narishige) under an inverted microscope at 20X magnification (Leica DM IRB/E, Germany).DNA was extracted from exponentially growing culture and sequence data from the chloroplast marker were deposited at the genbank with the accession numbers MW691103-06 for Aten-MI-2, Aten-MI-1, Amil-MI and Aten-WSY, respectively.Genetic characterisation of Symbiodiniaceae cultures and growth kinetics were compared under different thermal scenarios. After ~ 30 days incubation, four replicates of each strain were selleted and divided over 16 tubes. For full details, see Beltran et al., (2021).Maintenance and Update Frequency: notPlanned&rft.creator=Australian Institute of Marine Science (AIMS) &rft.date=2026&rft.coverage=westlimit=148.83546023567388; southlimit=-20.26278198266625; eastlimit=148.83546023567388; northlimit=-20.26278198266625&rft.coverage=westlimit=148.83546023567388; southlimit=-20.26278198266625; eastlimit=148.83546023567388; northlimit=-20.26278198266625&rft.coverage=westlimit=146.8497047367898; southlimit=-19.164088379859738; eastlimit=146.8497047367898; northlimit=-19.164088379859738&rft.coverage=westlimit=146.8497047367898; southlimit=-19.164088379859738; eastlimit=146.8497047367898; northlimit=-19.164088379859738&rft_rights=Creative Commons Attribution 3.0 Australia License http://creativecommons.org/licenses/by/3.0/au/&rft_rights=Use Limitation: All AIMS data, products and services are provided as is and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.&rft_rights=Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: Australian Institute of Marine Science (AIMS). (2022). Physiological diversity among sympatric, conspecific endosymbionts of coral (Cladocopium C1acro) from the Great Barrier Reef. https://apps.aims.gov.au/metadata/view/84609d81-15a3-47f5-9bb4-60a6d9557dd4, accessed[date-of-access].&rft_subject=oceans&rft.type=dataset&rft.language=English Access the data

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Use Limitation: All AIMS data, products and services are provided "as is" and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.

Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: "Australian Institute of Marine Science (AIMS). (2022). Physiological diversity among sympatric, conspecific endosymbionts of coral (Cladocopium C1acro) from the Great Barrier Reef. https://apps.aims.gov.au/metadata/view/84609d81-15a3-47f5-9bb4-60a6d9557dd4, accessed[date-of-access]".

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Full description

Strains of the common coral endosymbiont, Cladocopium C1acro were isolated from four Acropora corals collected from inshore sites on the Great Barrier Reef (Nelly Bay, Magnetic Island, and South Molle Island in the Whitsunday Islands). Colonies of Accropora Tenuis and Acropora millepora were growing at similar depths and photic habitats, between `~3-4m.

Before the culturing of the symbionts, freshly isolated cells from the host were genotyped. Clonal cultures were established using methods described in Beltrán, et.al. (2021).

For the generation of clonal cultures, two methods were used. Three of the four clonal cultures (Aten-MI-1, Aten-WSY, and Amil-MI) were obtained after plating the heterogeneous population onto IMK − 1% agar plus antibiotics and incubating them under the conditions as above and picking a single colony-forming unit. The fourth clonal culture (Aten-MI-2) was produced from a single cell isolated using a micro-manipulator (MN-153, Narishige) under an inverted microscope at 20X magnification (Leica DM IRB/E, Germany).

DNA was extracted from exponentially growing culture and sequence data from the chloroplast marker were deposited at the genbank with the accession numbers MW691103-06 for Aten-MI-2, Aten-MI-1, Amil-MI and Aten-WSY, respectively.

Genetic characterisation of Symbiodiniaceae cultures and growth kinetics were compared under different thermal scenarios. After ~ 30 days incubation, four replicates of each strain were selleted and divided over 16 tubes. For full details, see Beltran et al., (2021).

Lineage

Maintenance and Update Frequency: notPlanned

Notes

Credit
Puill-Stephan, E. (AIMS)

Credit
Howells, E. (AIMS) and University of Wollongong, NSW

Credit
Flores-Moya, A. Universidad de Malaga, Malaga, Spain

Credit
Doblin, M. University of Technology, NSW

Credit
Núñez-Lara, E. Universidad Autónoma del Carmen, Campeche, México

Credit
López, T. Universidad Autónoma del Carmenn, Campeche, México

Credit
van Oppen, MJH. (AIMS) and University of Melbourne, VIC

Credit
Beltrán, VH. Australian Institute of Marine Science (AIMS) and Universidad Autónoma del Carmen, Campeche, México

Modified: 25 09 2026

This dataset is part of a larger collection

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text: westlimit=148.83546023567388; southlimit=-20.26278198266625; eastlimit=148.83546023567388; northlimit=-20.26278198266625

text: westlimit=146.8497047367898; southlimit=-19.164088379859738; eastlimit=146.8497047367898; northlimit=-19.164088379859738

Subjects

oceans |

User Contributed Tags

Other Information

Beltrán, V.H., Puill-Stephan, E., Howells, E. et al. Physiological diversity among sympatric, conspecific endosymbionts of coral (Cladocopium C1acro) from the Great Barrier Reef. Coral Reefs 40, 985–997 (2021). https://doi.org/10.1007/s00338-021-02092-z

doi : https://doi.org/10.1007/s00338-021-02092-z DOI Link

GenBank Accession MW691103.1 Cladocopium sp. isolate Aten-MI-2 contains psbA minicircle non-coding region and photosystem II subunit D1 genomic sequence; chloroplast

url : https://www.ncbi.nlm.nih.gov/nuccore/MW691103 External Link

Identifiers

global : 84609d81-15a3-47f5-9bb4-60a6d9557dd4