Data

Periodic and continuous thermal selection of Durusdinium trenchii

Australian Institute of Marine Science
Australian Institute of Marine Science (AIMS)
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=https://apps.aims.gov.au/metadata/view/eceb7313-5c82-40c1-a341-1bf0564c873c&rft.title=Periodic and continuous thermal selection of Durusdinium trenchii&rft.identifier=https://apps.aims.gov.au/metadata/view/eceb7313-5c82-40c1-a341-1bf0564c873c&rft.publisher=Australian Institute of Marine Science (AIMS)&rft.description=We investigated how 1) the selection of a coral photoymbiont under fluctuating temperatures compared to continuous selection under elevated temperatures and 2) the frequency of temperature oscillations influenced adaptation.To this end, we exposed a Durususdinium trenchii culture (SCF086.01) to five temperatures profiles: diurnal fluctuations between an ambient and elevated temperature, fluctuations every 2-3 generations, fluctuations every 4-5 generations, continuous exposure to elevated temperatures and continuous exposure to an ambient temperature. We measured the growth performances of D. trenchii lineages to their respective selection strategies for 2.1 years. After 2.1 years of experimental evolution, we characterised the adaptive responses of the heat-evolved lineages at a range of temperatures (21-31°C) and contrasted these to wild-types. We measured growth rates, oxidative stress, photochemical efficiency, photosynthesis and respiration rates.This dataset contains the data collected during the experimental evolution and thermal performance assay. The experiments and measurements were carried out at the Symbiont Culture Facility at AIMS between 11.11.2020 and 20.04.2023.Maintenance and Update Frequency: notPlannedStatement: Cell density data was collected using a flow cytometer (BD Accuri™ C6 Plus Flow Cytometer, BD Biosciences). OD data was collected using a micrplate reader (Synergy H4, BioTek). Photochemical efficiency data (Fv/Fm and thylakoid membrane stability) was collected using an imaging pulse-amplitude modulation chlorophyll fluorometer (Maxi version IMAGING PAM M-Series, Walz). Reactive oxgen species levels were obtained by staining D. trenchii culture medium with CellROX Orange (Thermo Fisher Scientific). The stained samples were then processed using a a microplate reader (Synergy H4). Photosynthesis and respiration rates were measured using oxygen sensor spots (OXSP5-ADH, PyroScience) and an optical oxygen meter (FireSting, PyroScience).&rft.creator=Australian Institute of Marine Science (AIMS) &rft.date=2025&rft.coverage=westlimit=147.0559474825859; southlimit=-19.26810561378274; eastlimit=147.0559474825859; northlimit=-19.26810561378274&rft.coverage=westlimit=147.0559474825859; southlimit=-19.26810561378274; eastlimit=147.0559474825859; northlimit=-19.26810561378274&rft_rights=Creative Commons Attribution 3.0 Australia License http://creativecommons.org/licenses/by/3.0/au/&rft_rights=Use Limitation: All AIMS data, products and services are provided as is and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.&rft_rights=Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: Australian Institute of Marine Science (AIMS). (2023). Periodic and continuous thermal selection of Durusdinium trenchii. https://apps.aims.gov.au/metadata/view/eceb7313-5c82-40c1-a341-1bf0564c873c, accessed[date-of-access].&rft_subject=oceans&rft.type=dataset&rft.language=English Access the data
Cite Saved to MyRDA Save to MyRDA

Licence & Rights:

Open Licence view details
CC-BY

Creative Commons Attribution 3.0 Australia License
http://creativecommons.org/licenses/by/3.0/au/

Use Limitation: All AIMS data, products and services are provided "as is" and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.

Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: "Australian Institute of Marine Science (AIMS). (2023). Periodic and continuous thermal selection of Durusdinium trenchii. https://apps.aims.gov.au/metadata/view/eceb7313-5c82-40c1-a341-1bf0564c873c, accessed[date-of-access]".

Access:

Other

Full description

We investigated how 1) the selection of a coral photoymbiont under fluctuating temperatures compared to continuous selection under elevated temperatures and 2) the frequency of temperature oscillations influenced adaptation.


To this end, we exposed a Durususdinium trenchii culture (SCF086.01) to five temperatures profiles: diurnal fluctuations between an ambient and elevated temperature, fluctuations every 2-3 generations, fluctuations every 4-5 generations, continuous exposure to elevated temperatures and continuous exposure to an ambient temperature. We measured the growth performances of D. trenchii lineages to their respective selection strategies for 2.1 years. After 2.1 years of experimental evolution, we characterised the adaptive responses of the heat-evolved lineages at a range of temperatures (21-31°C) and contrasted these to wild-types. We measured growth rates, oxidative stress, photochemical efficiency, photosynthesis and respiration rates.


This dataset contains the data collected during the experimental evolution and thermal performance assay. The experiments and measurements were carried out at the Symbiont Culture Facility at AIMS between 11.11.2020 and 20.04.2023.

Lineage

Maintenance and Update Frequency: notPlanned
Statement: Cell density data was collected using a flow cytometer (BD Accuri™ C6 Plus Flow Cytometer, BD Biosciences). OD data was collected using a micrplate reader (Synergy H4, BioTek). Photochemical efficiency data (Fv/Fm and thylakoid membrane stability) was collected using an imaging pulse-amplitude modulation chlorophyll fluorometer (Maxi version IMAGING PAM M-Series, Walz). Reactive oxgen species levels were obtained by staining D. trenchii culture medium with CellROX Orange (Thermo Fisher Scientific). The stained samples were then processed using a a microplate reader (Synergy H4). Photosynthesis and respiration rates were measured using oxygen sensor spots (OXSP5-ADH, PyroScience) and an optical oxygen meter (FireSting, PyroScience).

Notes

Credit
Alvarez-Roa, C. AIMS
Credit
Chan, WY. The University of Melbourne
Credit
Buerger, P. Macquarie University
Credit
Reef Restoration and Adaptation Program (RRAP), Australia
Credit
Paul G. Allen Family Foundation, USA
Credit
Nitschke, MR. AIMS & Victoria University of Wellington
Credit
ARC Laureate Fellowship, No. FL180100036, Australian Research Council, Australia
Credit
Scharfenstein, HJ. Australian Institute of Marine Scienc (AIMS) & The University of Melbourne
Credit
van Oppen, MJH. AIMS & The University of Melbourne
Credit
Peplow, LM. AIMS

Modified: 19 09 2025

This dataset is part of a larger collection

Click to explore relationships graph
Help

Related People

147.05595,-19.26811

147.05594748259,-19.268105613783

text: westlimit=147.0559474825859; southlimit=-19.26810561378274; eastlimit=147.0559474825859; northlimit=-19.26810561378274

Subjects
oceans |

User Contributed Tags    

Login to tag this record with meaningful keywords to make it easier to discover

Other Information
Scharfenstein, H.J., Peplow, L.M., Alvarez-Roa, C., Nitschke, M.R., Chan, W.Y., Buerger, P. and van Oppen, M.J.H. (2024), Pushing the limits: expanding the temperature tolerance of a coral photosymbiont through differing selection regimes. New Phytol, 243: 2130-2145. https://doi.org/10.1111/nph.19996

doi : https://doi.org/10.1111/nph.19996

Physiological datasets - EE & TPA [xlsx file size: 38.5 MB]

uri : https://api.aims.gov.au/data-v2.0/eceb7313-5c82-40c1-a341-1bf0564c873c/files/Physiological datasets - EE & TPA.xlsx

Link to GitHub Repository - Code used for the analysis of physiological datasets associated with heat-evolved D. trenchii

uri : https://github.com/hscharfenstein/Periodic-and-continuous-thermal-selection-of-D.trenchii

Identifiers
  • global : eceb7313-5c82-40c1-a341-1bf0564c873c
ACN 633 798 857