Data

Marine eukaryotes and bacteria, Antarctica

Name: Marine eukaryotes and bacteria, Antarctica
Published: 2026

Australian Institute of Marine Science

Australian Institute of Marine Science (AIMS)

Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]

ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=https://apps.aims.gov.au/metadata/view/db3eae60-19b1-48bd-96a2-22bf4dd3ce47&rft.title=Marine eukaryotes and bacteria, Antarctica&rft.identifier=https://apps.aims.gov.au/metadata/view/db3eae60-19b1-48bd-96a2-22bf4dd3ce47&rft.publisher=Australian Institute of Marine Science (AIMS)&rft.description=Glass slides were deployed at 12m and 18m3 at sites in McMurdo Sound (adjacent to McMurdo Station, Scott Base and Cape Armitage) for a period of 12 months to collect biofilm.18S rDNA sequence information was obtained for representatives of each DGGE (denaturing gradient gel electrophoresis) band at each site and depth. This data was interrogated to phyla level: cnidaria, bryozoa, protozoa, dinoflagellates, arthropods, platyhelminths and annelids were present.Density of diatoms observed by fluorescence microscopy for each site and depth were recorded (per 100 µm2). At least 6 different diatom morphotypes were identified based on size and morphology.The slides were also examined by performing fluorescence in situ hybridization (FISH) reactions with bacteria-specific probes (Eub338), an archaea-specific probe (ARCH915) and 9 different bacterial group-specific probes. Bacterial groups identified from the sequences were: Gammaproteobacteria, SRB, Planctomycetales, Alphaproteobacteria, Archaea, Betaproteobacteria, Actinobacteria and Firmicutes, Cytophaga /Flavobacteria of Bacteroidetes (CFB), Verrucomicrobia.This study was the first to examine eukaryotic recruitment, potentially overlooked in visual surveys, using molecular techniques. To use molecular techniques to examine the community structure of marine eukaryotes and bacteria recruiting onto artificial substrata.Maintenance and Update Frequency: notPlanned&rft.creator=Australian Institute of Marine Science (AIMS) &rft.date=2026&rft.coverage=westlimit=166.6732; southlimit=-77.863; eastlimit=166.6732; northlimit=-77.863&rft.coverage=westlimit=166.6732; southlimit=-77.863; eastlimit=166.6732; northlimit=-77.863&rft.coverage=westlimit=166.75; southlimit=-77.855; eastlimit=166.75; northlimit=-77.855&rft.coverage=westlimit=166.75; southlimit=-77.855; eastlimit=166.75; northlimit=-77.855&rft.coverage=westlimit=166.652; southlimit=-77.857; eastlimit=166.652; northlimit=-77.857&rft.coverage=westlimit=166.652; southlimit=-77.857; eastlimit=166.652; northlimit=-77.857&rft_rights=Creative Commons Attribution-NonCommercial 3.0 Australia License http://creativecommons.org/licenses/by-nc/3.0/au/&rft_rights=Use Limitation: All AIMS data, products and services are provided as is and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.&rft_rights=Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: Australian Institute of Marine Science (AIMS). (2009). Marine eukaryotes and bacteria, Antarctica. https://apps.aims.gov.au/metadata/view/db3eae60-19b1-48bd-96a2-22bf4dd3ce47, accessed[date-of-access].&rft_rights=Resource Usage:Use of the AIMS data is for not-for-profit applications only. All other users shall seek permission for use by contacting AIMS. Acknowledgements as prescribed must be clearly set out in the user's formal communications or publications.Access Constraint: intellectualPropertyRightsUse Constraint: intellectualPropertyRightsSecurity classification code: unclassifiedMetadata Usage:Access Constraint: intellectualPropertyRightsUse Constraint: intellectualPropertyRightsSecurity classification code: unclassified&rft_subject=oceans&rft.type=dataset&rft.language=English Access the data

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Creative Commons Attribution-NonCommercial 3.0 Australia License
http://creativecommons.org/licenses/by-nc/3.0/au/

Use Limitation: All AIMS data, products and services are provided "as is" and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.

Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: "Australian Institute of Marine Science (AIMS). (2009). Marine eukaryotes and bacteria, Antarctica. https://apps.aims.gov.au/metadata/view/db3eae60-19b1-48bd-96a2-22bf4dd3ce47, accessed[date-of-access]".

Resource Usage:Use of the AIMS data is for not-for-profit applications only. All other users shall seek permission for use by contacting AIMS. Acknowledgements as prescribed must be clearly set out in the user's formal communications or publications.Access Constraint: intellectualPropertyRightsUse Constraint: intellectualPropertyRightsSecurity classification code: unclassifiedMetadata Usage:Access Constraint: intellectualPropertyRightsUse Constraint: intellectualPropertyRightsSecurity classification code: unclassified

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Full description

Glass slides were deployed at 12m and 18m3 at sites in McMurdo Sound (adjacent to McMurdo Station, Scott Base and Cape Armitage) for a period of 12 months to collect biofilm.18S rDNA sequence information was obtained for representatives of each DGGE (denaturing gradient gel electrophoresis) band at each site and depth. This data was interrogated to phyla level: cnidaria, bryozoa, protozoa, dinoflagellates, arthropods, platyhelminths and annelids were present.Density of diatoms observed by fluorescence microscopy for each site and depth were recorded (per 100 µm2). At least 6 different diatom morphotypes were identified based on size and morphology.The slides were also examined by performing fluorescence in situ hybridization (FISH) reactions with bacteria-specific probes (Eub338), an archaea-specific probe (ARCH915) and 9 different bacterial group-specific probes. Bacterial groups identified from the sequences were: Gammaproteobacteria, SRB, Planctomycetales, Alphaproteobacteria, Archaea, Betaproteobacteria, Actinobacteria and Firmicutes, Cytophaga /Flavobacteria of Bacteroidetes (CFB), Verrucomicrobia.This study was the first to examine eukaryotic recruitment, potentially overlooked in visual surveys, using molecular techniques.
To use molecular techniques to examine the community structure of marine eukaryotes and bacteria recruiting onto artificial substrata.

Lineage

Maintenance and Update Frequency: notPlanned

Notes

Credit
Webster, Nicole S, Dr (Principal Investigator)

Modified: 11 09 2026

This dataset is part of a larger collection

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text: westlimit=166.6732; southlimit=-77.863; eastlimit=166.6732; northlimit=-77.863

text: westlimit=166.75; southlimit=-77.855; eastlimit=166.75; northlimit=-77.855

text: westlimit=166.652; southlimit=-77.857; eastlimit=166.652; northlimit=-77.857

Subjects

oceans |

User Contributed Tags

Other Information

Site-specific variation in Antarctic marine biofilms established on artificial surfaces: Webster NS and Negri AP (2006) Site-specific variation in Antarctic marine biofilms established on artificial surfaces. Environmental Microbiology 8: 1177-1190.

local : articleId=7159

Recruitment of Antarctic marine eukaryotes onto artificial surfaces: Webster NS, Battershill CN and Negri AP (2006) Recruitment of Antarctic marine eukaryotes onto artificial surfaces. Polar Biology 30: 1-10.

local : articleId=7222

Identifiers

global : db3eae60-19b1-48bd-96a2-22bf4dd3ce47