Data

Interactive effects of copper and temperature on coral larvae fom the Great Barrier Reef

Name: Interactive effects of copper and temperature on coral larvae fom the Great Barrier Reef
Published: 2026

Australian Institute of Marine Science

Australian Institute of Marine Science (AIMS)

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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=https://apps.aims.gov.au/metadata/view/0eb44196-fccc-45be-9ab8-830c355adf97&rft.title=Interactive effects of copper and temperature on coral larvae fom the Great Barrier Reef&rft.identifier=https://apps.aims.gov.au/metadata/view/0eb44196-fccc-45be-9ab8-830c355adf97&rft.publisher=Australian Institute of Marine Science (AIMS)&rft.description=Gravid colonies of the corals Acropora millepora and Acropora tenuis were collected from Magnetic Island and transported to the outdoor aquaria at the Australian Institute of Marine Science. The gametes were collected after spawning and the azooxanthellate larvae were cultured in indoor aquaria at 27°C.Seven day old larvae were added to culture plates containing copper solutions with concentrations of 0, 1, 2, 4, 8, 12, 16, 24, 32 and 64 µg/l and incubated at 27, 30, 31, 32, 33 and 34°C under light conditions set at 30 µmol quanta/m²/s. Six replicates were used for each of the 60 treatments.After incubation for 6 hours, larval metamorphosis was initiated by the addition of extracts derived from two species of crustose coralline algae, Neogoniolithon fosliei and Porolithon onkodes. After a further 18 hours, treatments were terminated and larvae fixed for later assessment of settlement and metamorphosis. This research was undertaken to collect data to provide parameters for a model of the effects of temperature and copper contamination on larval metamorphosis.Maintenance and Update Frequency: notPlannedStatement: Statement: The coral larvae were cultured using the methods described in:Negri AP, Heyward AJ (2000) Inhibition of fertilization and larval metamorphosis of the coral Acropora millepora (Ehrenberg, 1834) by petroleum products. Mar Pollut Bull 41: 420-427.Stock Cu solutions were prepared individually in MilliQ water using CuCl2 (Sigma). Secondary stock solutions (10 x higher than final concentrations) were then prepared in 0.45 µm filtered seawater (FSW) and pHs adjusted to 8.2 with NaOH. Identical solutions were prepared and sampled for Cu analysis at Charles Darwin University by ICP- MS (Agilent 750ce) using a seawater standard additions method. Quality control for the analysis included NASS-5 and CASS-4 Certified Reference Materials, inhouse reference materials and spike recoveries. To control for potential differences in water quality parameters between treatments, dissolved oxygen (mg/L) and pH were also measured at selected temperatures and Cu concentrations at t =0 and t =24h for treatments containing 10 day old Acropora millepora larvae.Extracts were prepared from the crustose coralline algae Neogoniolithon fosliei and Porolithon onkodes using the method described in:Heyward AJ, Negri AP (1999) Natural inducers for coral larval metamorphosis. Coral Reefs 18: 273-279.&rft.creator=Australian Institute of Marine Science (AIMS) &rft.date=2026&rft.coverage=westlimit=146.847684; southlimit=-19.171696; eastlimit=146.847684; northlimit=-19.171696&rft.coverage=westlimit=146.847684; southlimit=-19.171696; eastlimit=146.847684; northlimit=-19.171696&rft_rights=Creative Commons Attribution-NonCommercial 3.0 Australia License http://creativecommons.org/licenses/by-nc/3.0/au/&rft_rights=Use Limitation: All AIMS data, products and services are provided as is and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.&rft_rights=Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: Australian Institute of Marine Science (AIMS). (2011). Interactive effects of copper and temperature on coral larvae fom the Great Barrier Reef. https://apps.aims.gov.au/metadata/view/0eb44196-fccc-45be-9ab8-830c355adf97, accessed[date-of-access].&rft_rights=Resource Usage:Use of the AIMS data is for not-for-profit applications only. All other users shall seek permission for use by contacting AIMS. Acknowledgements as prescribed must be clearly set out in the user's formal communications or publications.&rft_subject=oceans&rft.type=dataset&rft.language=English Access the data

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Creative Commons Attribution-NonCommercial 3.0 Australia License
http://creativecommons.org/licenses/by-nc/3.0/au/

Use Limitation: All AIMS data, products and services are provided "as is" and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.

Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: "Australian Institute of Marine Science (AIMS). (2011). Interactive effects of copper and temperature on coral larvae fom the Great Barrier Reef. https://apps.aims.gov.au/metadata/view/0eb44196-fccc-45be-9ab8-830c355adf97, accessed[date-of-access]".

Resource Usage:Use of the AIMS data is for not-for-profit applications only. All other users shall seek permission for use by contacting AIMS. Acknowledgements as prescribed must be clearly set out in the user's formal communications or publications.

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Gravid colonies of the corals Acropora millepora and Acropora tenuis were collected from Magnetic Island and transported to the outdoor aquaria at the Australian Institute of Marine Science. The gametes were collected after spawning and the azooxanthellate larvae were cultured in indoor aquaria at 27°C.Seven day old larvae were added to culture plates containing copper solutions with concentrations of 0, 1, 2, 4, 8, 12, 16, 24, 32 and 64 µg/l and incubated at 27, 30, 31, 32, 33 and 34°C under light conditions set at 30 µmol quanta/m²/s. Six replicates were used for each of the 60 treatments.After incubation for 6 hours, larval metamorphosis was initiated by the addition of extracts derived from two species of crustose coralline algae, Neogoniolithon fosliei and Porolithon onkodes. After a further 18 hours, treatments were terminated and larvae fixed for later assessment of settlement and metamorphosis.
This research was undertaken to collect data to provide parameters for a model of the effects of temperature and copper contamination on larval metamorphosis.

Lineage

Maintenance and Update Frequency: notPlanned

Statement: Statement: The coral larvae were cultured using the methods described in:Negri AP, Heyward AJ (2000) Inhibition of fertilization and larval metamorphosis of the coral Acropora millepora (Ehrenberg, 1834) by petroleum products. Mar Pollut Bull 41: 420-427.Stock Cu solutions were prepared individually in MilliQ water using CuCl2 (Sigma). Secondary stock solutions (10 x higher than final concentrations) were then prepared in 0.45 µm filtered seawater (FSW) and pHs adjusted to 8.2 with NaOH. Identical solutions were prepared and sampled for Cu analysis at Charles Darwin University by ICP- MS (Agilent 750ce) using a seawater standard additions method. Quality control for the analysis included NASS-5 and CASS-4 Certified Reference Materials, inhouse reference materials and spike recoveries. To control for potential differences in water quality parameters between treatments, dissolved oxygen (mg/L) and pH were also measured at selected temperatures and Cu concentrations at t =0 and t =24h for treatments containing 10 day old Acropora millepora larvae.Extracts were prepared from the crustose coralline algae Neogoniolithon fosliei and Porolithon onkodes using the method described in:Heyward AJ, Negri AP (1999) Natural inducers for coral larval metamorphosis. Coral Reefs 18: 273-279.

Notes

Credit
Negri, Andrew P, Dr (Principal Investigator)

Modified: 18 09 2026

This dataset is part of a larger collection

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text: westlimit=146.847684; southlimit=-19.171696; eastlimit=146.847684; northlimit=-19.171696

Subjects

oceans |

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Other Information

Water contamination reduces the tolerance of coral larvae to thermal stress: Negri AP and Hoogenboom M (2011) Water contamination reduces the tolerance of coral larvae to thermal stress. PLoS ONE 6: e19703.

local : articleId=8820

Identifiers

global : 0eb44196-fccc-45be-9ab8-830c355adf97