Full description
Extracellular vesicles were isolated using EXO-NET, and the RNA content extracted using the Norgen Exosomal RNA Isolation Kit. Afterwards, the RNA was processed to generate small RNA libraries, using the NEXTFLEX® Small RNA-seq v3 Kit with UDI's. The libraries were quantified using the KAPA Library Quantification Kit and libraries pooled in equimolar quantities. The pooled library was sequenced with the following conditions, single index 8bp read and 76bp for read 1, using High Output 75 cycle kit on NextSeq 500.Issued: 09 06 2023
Subjects
Biological Sciences |
Biomedical and Clinical Sciences |
Bioinformatics and Computational Biology |
eng |
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Other Information
Identifiers
- Local : RDM ID: 753633c0-0679-11ee-957e-bf9b55e47b8e
- DOI : 10.48610/C517A74
