Identification of nuclear reprogramming factors in oocyte cytoplasm [ 2002-01-01 - 2007-12-31 ]

Research Grant

Researchers: Dr Andrew French (Chief Investigator) ,  Prof Alan Trounson (Chief Investigator)

Brief description Identification of nuclear reprogramming factors in oocyte cytoplasm. The mature oocyte contains dominant factors that are capable of erasing tissue specific gene expression profiles of somatic cells. These reprogramming factors would be valuable for dedifferentiation of cells and for nuclear transfer in animal cloning. The research involves determination of reprogramming factors present in active cytoplasm following enucleation of the germinal vesicle, blockage of transcription and translation, and timed cultures. The assays will involve maintenance of reprogramming ability and erasure of somatic gene transcription. By subtractive elimination the function of isolated proteins which are involved in reprogramming will be identified for potential recombinant production.

Funding Amount $67,635

Funding Scheme Linkage Projects

View this grant in the ARC Data Portal