Data

Haemolymph protein in xanthid crabs from the Great Barrier Reef

Australian Institute of Marine Science
Australian Institute of Marine Science (AIMS)
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=http://geo.aims.gov.au/geonetwork/geonetwork/srv/eng/search?uuid=dcb114cc-1fe9-4d4e-8d55-0b24c675e38c&rft.title=Haemolymph protein in xanthid crabs from the Great Barrier Reef&rft.identifier=http://geo.aims.gov.au/geonetwork/geonetwork/srv/eng/search?uuid=dcb114cc-1fe9-4d4e-8d55-0b24c675e38c&rft.publisher=Australian Institute of Marine Science (AIMS)&rft.description=Haemolymph was obtained from 26 xanthid crabs collected at low tide from Reef 21-145 and Laver's Cay on the Great Barrier Reef. A combinatin of protease inhibitors (5 mM EDTA, 1 µM pepstatin, 1 µM aprotonin and 100 µM phenylmethylsulfonyl fluoride) was added to the haemolymph. Measurements of equilibrium 3H-STX-binding, time courses of dissociation and association, and the pH-dependence of 3H-STX-binding was conducted on the crabs. Species were: Lophozozymus pictor (2), Liomera tristis (3), Chlorodiella nigra (2), Actaeodes tomentosus (2), Atergatis floridus (15), and Platypodia granulosa (2).Rat-brain synaptosomes were prepared from postmortem rat-brain tissue and binding assays conducted. Tissue extracted from each crab tested for 3H-STX-binding activity was then assayed for paralytic shellfish toxin (PST) activity by rat synaptosome. To report the discovery of a soluble and apparently proteinaceous 3H-STX-binding factor in the haemolymph of several species of xanthid crabs.To describe the attributes of this haemolymph factor in relation to saxiphilin and the sodium channel and to test its ability to bind tetrodotoxin (TTX) and saxitoxin (STX).Maintenance and Update Frequency: notPlanned&rft.creator=Australian Institute of Marine Science (AIMS) &rft.date=2024&rft.coverage=westlimit=169.26472; southlimit=-19.535; eastlimit=169.26472; northlimit=-19.535&rft.coverage=westlimit=169.26472; southlimit=-19.535; eastlimit=169.26472; northlimit=-19.535&rft_rights= http://creativecommons.org/licenses/by-nc/3.0/au/&rft_rights=http://i.creativecommons.org/l/by-nc/3.0/au/88x31.png&rft_rights=WWW:LINK-1.0-http--related&rft_rights=License Graphic&rft_rights=Creative Commons Attribution-NonCommercial 3.0 Australia License&rft_rights=http://creativecommons.org/international/au/&rft_rights=WWW:LINK-1.0-http--related&rft_rights=WWW:LINK-1.0-http--related&rft_rights=License Text&rft_rights=Use Limitation: All AIMS data, products and services are provided as is and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.&rft_rights=Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: Australian Institute of Marine Science (AIMS). (2009). Haemolymph protein in xanthid crabs from the Great Barrier Reef. https://apps.aims.gov.au/metadata/view/dcb114cc-1fe9-4d4e-8d55-0b24c675e38c, accessed[date-of-access].&rft_rights=Resource Usage:Use of the AIMS data is for not-for-profit applications only. All other users shall seek permission for use by contacting AIMS. Acknowledgements as prescribed must be clearly set out in the user's formal communications or publications.&rft_rights=Creative Commons Attribution-NonCommercial 3.0 Australia License http://creativecommons.org/licenses/by-nc/3.0/au&rft_subject=oceans&rft.type=dataset&rft.language=English Access the data

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Use Limitation: All AIMS data, products and services are provided "as is" and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.

Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: "Australian Institute of Marine Science (AIMS). (2009). Haemolymph protein in xanthid crabs from the Great Barrier Reef. https://apps.aims.gov.au/metadata/view/dcb114cc-1fe9-4d4e-8d55-0b24c675e38c, accessed[date-of-access]".

Resource Usage:Use of the AIMS data is for not-for-profit applications only. All other users shall seek permission for use by contacting AIMS. Acknowledgements as prescribed must be clearly set out in the user's formal communications or publications.

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Contact Information

adc@aims.gov.au

Brief description

Haemolymph was obtained from 26 xanthid crabs collected at low tide from Reef 21-145 and Laver's Cay on the Great Barrier Reef. A combinatin of protease inhibitors (5 mM EDTA, 1 µM pepstatin, 1 µM aprotonin and 100 µM phenylmethylsulfonyl fluoride) was added to the haemolymph. Measurements of equilibrium 3H-STX-binding, time courses of dissociation and association, and the pH-dependence of 3H-STX-binding was conducted on the crabs. Species were: Lophozozymus pictor (2), Liomera tristis (3), Chlorodiella nigra (2), Actaeodes tomentosus (2), Atergatis floridus (15), and Platypodia granulosa (2).Rat-brain synaptosomes were prepared from postmortem rat-brain tissue and binding assays conducted. Tissue extracted from each crab tested for 3H-STX-binding activity was then assayed for paralytic shellfish toxin (PST) activity by rat synaptosome.
To report the discovery of a soluble and apparently proteinaceous 3H-STX-binding factor in the haemolymph of several species of xanthid crabs.To describe the attributes of this haemolymph factor in relation to saxiphilin and the sodium channel and to test its ability to bind tetrodotoxin (TTX) and saxitoxin (STX).

Lineage

Maintenance and Update Frequency: notPlanned

Notes

Credit
Llewellyn, Lyndon E, Dr (Principal Investigator)

Modified: 13 03 2024

This dataset is part of a larger collection

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169.26472,-19.535

169.26472,-19.535

text: westlimit=169.26472; southlimit=-19.535; eastlimit=169.26472; northlimit=-19.535

Subjects
oceans |

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Other Information
Haemolymph protein in xanthid crabs: its selective binding of saxitoxin and possible role in toxin bioaccumulation: Llewellyn LE (1997) Haemolymph protein in xanthid crabs: its selective binding of saxitoxin and possible role in toxin bioaccumulation. Marine Biology 128: 599-606.

local : articleId=1498

Background information on crab toxicity

uri : http://www.aims.gov.au/pages/about/communications/backgrounders/20020905-killer-crabs.html

Identifiers
  • global : dcb114cc-1fe9-4d4e-8d55-0b24c675e38c