Data

Gene flow between populations of Holothura nobilis on the Great Barrier Reef

Australian Institute of Marine Science
Australian Institute of Marine Science (AIMS)
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=http://geo.aims.gov.au/geonetwork/geonetwork/srv/eng/search?uuid=90bbf04e-86ce-4511-bef8-1ddbeab71a01&rft.title=Gene flow between populations of Holothura nobilis on the Great Barrier Reef&rft.identifier=http://geo.aims.gov.au/geonetwork/geonetwork/srv/eng/search?uuid=90bbf04e-86ce-4511-bef8-1ddbeab71a01&rft.publisher=Australian Institute of Marine Science (AIMS)&rft.description=Genetic variation was studied at 7 polymorphic allozyme loci in 15 Holothuria nobilis (now referred to as H. whitmaei) populations on the Great Barrier Reef. The 7 polymorphic enzyme loci surveyed using allozyme electrophoresis were: FL-EST, GPI, HK, MDH, MPI, PGM, TPI. Full details of staining and electrophoresis methods are given in:Ballment E, Uthicke S, Peplow L, Benzie JAH (1997) Techniques for enzyme electrophoretic analysis of the holothurians Holothuria atra and Stichopus chloronotus (Holothuroidea: Aspidochirotida). Aust Inst Mar Sci (AIMS) Tech Rep Ser 27:1-47Basic analyses of genetic variability were carried out using programs in the BIOSYS-1. F-statistics, cluster analyses and tests of conformation to Hardy-Weinberg expectations were performed using the TFPGA package. To infer the level of dispersal among populations of Holothuria nobilis separated by up to 1300 km along the Great Barrier Reef by investigating gene flow.To test whether asexual reproduction is a feature of this species. Reef locations: 13-050, 21-149, 21-151, Big Broadhurst, Davie, Davies Reef, East Cay, Hicks, Little Broadhurst, Michaelmas, Opal, Ribbon No.10, Stucco, Turner, White Tip.Maintenance and Update Frequency: notPlannedStatement: Statement: Techniques are delineated in:Ballment E, Uthicke S, Peplow L and Benzie JAH (1997) Techniques for enzyme electrophoretic analysis of the holothurians, Holothuria atra and Stichopus chloronotus (Holothuroidea: Aspidochirotida. AIMS report no. 27. Australian Institute of Marine Science. 47 p.&rft.creator=Australian Institute of Marine Science (AIMS) &rft.date=2024&rft.coverage=westlimit=143.9; southlimit=-21.8; eastlimit=152.6; northlimit=-13.3&rft.coverage=westlimit=143.9; southlimit=-21.8; eastlimit=152.6; northlimit=-13.3&rft_rights= http://creativecommons.org/licenses/by-nc/3.0/au/&rft_rights=http://i.creativecommons.org/l/by-nc/3.0/au/88x31.png&rft_rights=WWW:LINK-1.0-http--related&rft_rights=License Graphic&rft_rights=Creative Commons Attribution-NonCommercial 3.0 Australia License&rft_rights=http://creativecommons.org/international/au/&rft_rights=WWW:LINK-1.0-http--related&rft_rights=WWW:LINK-1.0-http--related&rft_rights=License Text&rft_rights=Use Limitation: All AIMS data, products and services are provided as is and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.&rft_rights=Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: Australian Institute of Marine Science (AIMS). (2009). Gene flow between populations of Holothura nobilis on the Great Barrier Reef. https://apps.aims.gov.au/metadata/view/90bbf04e-86ce-4511-bef8-1ddbeab71a01, accessed[date-of-access].&rft_rights=Resource Usage:Use of the AIMS data is for not-for-profit applications only. All other users shall seek permission for use by contacting AIMS. Acknowledgements as prescribed must be clearly set out in the user's formal communications or publications.Access Constraint: intellectualPropertyRightsUse Constraint: intellectualPropertyRightsSecurity classification code: unclassifiedMetadata Usage:Access Constraint: intellectualPropertyRightsUse Constraint: intellectualPropertyRightsSecurity classification code: unclassified&rft_rights=Creative Commons Attribution-NonCommercial 3.0 Australia License http://creativecommons.org/licenses/by-nc/3.0/au&rft_subject=oceans&rft.type=dataset&rft.language=English Access the data
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Use Limitation: All AIMS data, products and services are provided "as is" and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.

Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: "Australian Institute of Marine Science (AIMS). (2009). Gene flow between populations of Holothura nobilis on the Great Barrier Reef. https://apps.aims.gov.au/metadata/view/90bbf04e-86ce-4511-bef8-1ddbeab71a01, accessed[date-of-access]".

Resource Usage:Use of the AIMS data is for not-for-profit applications only. All other users shall seek permission for use by contacting AIMS. Acknowledgements as prescribed must be clearly set out in the user's formal communications or publications.Access Constraint: intellectualPropertyRightsUse Constraint: intellectualPropertyRightsSecurity classification code: unclassifiedMetadata Usage:Access Constraint: intellectualPropertyRightsUse Constraint: intellectualPropertyRightsSecurity classification code: unclassified

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adc@aims.gov.au

Brief description

Genetic variation was studied at 7 polymorphic allozyme loci in 15 Holothuria nobilis (now referred to as H. whitmaei) populations on the Great Barrier Reef. The 7 polymorphic enzyme loci surveyed using allozyme electrophoresis were: FL-EST, GPI, HK, MDH, MPI, PGM, TPI. Full details of staining and electrophoresis methods are given in:Ballment E, Uthicke S, Peplow L, Benzie JAH (1997) Techniques for enzyme electrophoretic analysis of the holothurians Holothuria atra and Stichopus chloronotus (Holothuroidea: Aspidochirotida). Aust Inst Mar Sci (AIMS) Tech Rep Ser 27:1-47Basic analyses of genetic variability were carried out using programs in the BIOSYS-1. F-statistics, cluster analyses and tests of conformation to Hardy-Weinberg expectations were performed using the TFPGA package.
To infer the level of dispersal among populations of Holothuria nobilis separated by up to 1300 km along the Great Barrier Reef by investigating gene flow.To test whether asexual reproduction is a feature of this species.
Reef locations: 13-050, 21-149, 21-151, Big Broadhurst, Davie, Davies Reef, East Cay, Hicks, Little Broadhurst, Michaelmas, Opal, Ribbon No.10, Stucco, Turner, White Tip.

Lineage

Maintenance and Update Frequency: notPlanned
Statement: Statement: Techniques are delineated in:Ballment E, Uthicke S, Peplow L and Benzie JAH (1997) Techniques for enzyme electrophoretic analysis of the holothurians, Holothuria atra and Stichopus chloronotus (Holothuroidea: Aspidochirotida. AIMS report no. 27. Australian Institute of Marine Science. 47 p.

Notes

Credit
Uthicke, Sven, Dr (Principal Investigator)

Modified: 13 03 2024

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152.6,-13.3 152.6,-21.8 143.9,-21.8 143.9,-13.3 152.6,-13.3

148.25,-17.55

text: westlimit=143.9; southlimit=-21.8; eastlimit=152.6; northlimit=-13.3

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Other Information
Allozyme electrophoresis indicates high gene flow between populations of Holothura (Microthele) nobilis (Holothuroidea: Aspidochirotida) on the Great Barrier Reef: Uthicke S and Benzie JAH (2000) Allozyme electrophoresis indicates high gene flow between populations of Holothura (Microthele) nobilis (Holothuroidea: Aspidochirotida) on the Great Barrier Reef. Marine Biology 137: 819-825.

local : articleId=3551

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  • global : 90bbf04e-86ce-4511-bef8-1ddbeab71a01
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