Data

Gauging the effects of shellfish extract matrices, salts and pH upon performance of an assay for paralytic shellfish toxins using saxiphilin

Australian Institute of Marine Science
Australian Institute of Marine Science (AIMS)
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=http://geo.aims.gov.au/geonetwork/geonetwork/srv/eng/search?uuid=a7ec565a-beec-4269-90ad-508c93b68ed0&rft.title=Gauging the effects of shellfish extract matrices, salts and pH upon performance of an assay for paralytic shellfish toxins using saxiphilin&rft.identifier=http://geo.aims.gov.au/geonetwork/geonetwork/srv/eng/search?uuid=a7ec565a-beec-4269-90ad-508c93b68ed0&rft.publisher=Australian Institute of Marine Science (AIMS)&rft.description=In this study, a microtitre plate binding assay using a saxiphilin isoform [3H]STX from the centipede Ethmostigmus rubripes, was challenged with differing conditions of salt concentration and identity, pH and the addition of non-toxic extracts of commercial shellfish.Elements present (Na, K, Mg, Ca, Zn, Ni, Mn, Cr, Sr, Mo, Al, V, Fe, Cu, Pb, Co) in the shellfish extracts (green lipped mussel, Perna canalicularis; Sydney rock oyster, Saccostrea glomerata; and pipis, Donax deltoides) were measured by inductively coupled plasma spectroscopy. The effects upon the assay by the four most common salts of these elements, NaCl, KCl, CaCl2 and MgCl2 were studied by varying their concentrations (0-800 mM).The effect of pH upon the assay was measured by replacing the assay buffer in standard conditions with a buffer mixture of Tris (20 mM) -Mes (10 mM) -acetic acid (10 mM), adjusted with tetramethylammonium hydroxide or HCl to indicated pH which ranged from 3.4 to 9.0. To investigate the microtitre plate saxiphilin assay's ability to deal with potential interfering compounds from shellfish extracts, and the effect of acidic pH necessary for toxin stability when extracting PSTs from shellfish.Maintenance and Update Frequency: notPlanned&rft.creator=Australian Institute of Marine Science (AIMS) &rft.date=2024&rft_rights= http://creativecommons.org/licenses/by-nc/3.0/au/&rft_rights=http://i.creativecommons.org/l/by-nc/3.0/au/88x31.png&rft_rights=WWW:LINK-1.0-http--related&rft_rights=License Graphic&rft_rights=Creative Commons Attribution-NonCommercial 3.0 Australia License&rft_rights=http://creativecommons.org/international/au/&rft_rights=WWW:LINK-1.0-http--related&rft_rights=WWW:LINK-1.0-http--related&rft_rights=License Text&rft_rights=Use Limitation: All AIMS data, products and services are provided as is and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.&rft_rights=Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: Australian Institute of Marine Science (AIMS). (2009). Gauging the effects of shellfish extract matrices, salts and pH upon performance of an assay for paralytic shellfish toxins using saxiphilin. https://apps.aims.gov.au/metadata/view/a7ec565a-beec-4269-90ad-508c93b68ed0, accessed[date-of-access].&rft_rights=Resource Usage:Use of the AIMS data is for not-for-profit applications only. All other users shall seek permission for use by contacting AIMS. Acknowledgements as prescribed must be clearly set out in the user's formal communications or publications.&rft_rights=Creative Commons Attribution-NonCommercial 3.0 Australia License http://creativecommons.org/licenses/by-nc/3.0/au&rft_subject=oceans&rft.type=dataset&rft.language=English Access the data

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Use Limitation: All AIMS data, products and services are provided "as is" and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.

Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: "Australian Institute of Marine Science (AIMS). (2009). Gauging the effects of shellfish extract matrices, salts and pH upon performance of an assay for paralytic shellfish toxins using saxiphilin. https://apps.aims.gov.au/metadata/view/a7ec565a-beec-4269-90ad-508c93b68ed0, accessed[date-of-access]".

Resource Usage:Use of the AIMS data is for not-for-profit applications only. All other users shall seek permission for use by contacting AIMS. Acknowledgements as prescribed must be clearly set out in the user's formal communications or publications.

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Brief description

In this study, a microtitre plate binding assay using a saxiphilin isoform [3H]STX from the centipede Ethmostigmus rubripes, was challenged with differing conditions of salt concentration and identity, pH and the addition of non-toxic extracts of commercial shellfish.Elements present (Na, K, Mg, Ca, Zn, Ni, Mn, Cr, Sr, Mo, Al, V, Fe, Cu, Pb, Co) in the shellfish extracts (green lipped mussel, Perna canalicularis; Sydney rock oyster, Saccostrea glomerata; and pipis, Donax deltoides) were measured by inductively coupled plasma spectroscopy. The effects upon the assay by the four most common salts of these elements, NaCl, KCl, CaCl2 and MgCl2 were studied by varying their concentrations (0-800 mM).The effect of pH upon the assay was measured by replacing the assay buffer in standard conditions with a buffer mixture of Tris (20 mM) -Mes (10 mM) -acetic acid (10 mM), adjusted with tetramethylammonium hydroxide or HCl to indicated pH which ranged from 3.4 to 9.0.
To investigate the microtitre plate saxiphilin assay's ability to deal with potential interfering compounds from shellfish extracts, and the effect of acidic pH necessary for toxin stability when extracting PSTs from shellfish.

Lineage

Maintenance and Update Frequency: notPlanned

Notes

Credit
Llewellyn, Lyndon E, Dr (Principal Investigator)

Modified: 13 03 2024

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Other Information
Microtitre plate assay for paralytic shellfish toxins using saxiphilin: gauging the effects of shellfish extract matrices, salts and pH upon assay performance: Llewellyn LE and Doyle JR (2001) Microtitre plate assay for paralytic shellfish toxins using saxiphilin: gauging the effects of shellfish extract matrices, salts and pH upon assay performance. Toxicon 39: 217-224.

local : articleId=1720

Identifiers
  • global : a7ec565a-beec-4269-90ad-508c93b68ed0