Data

EDNA data for Crown of Thorns Seastar (Acanthaster cf. solaris) - CCIP-RT-02

Australian Institute of Marine Science
Australian Institute of Marine Science (AIMS)
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
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Data DictionaryField name: Explanation/unitsDate: Julian DateYear: Collection YearReef: Reef NameReefName: Official GBRMPA Reef name and IDSite_name: Site nameSite_No: Site numberLat: Latitude in decimal degreesLong: Longitude in decimal degreesProject: Project CodeCollection organisation: Organisation that conducted the primary collectionCollection vessel: Vessel from which samples were collectedReef_rep: replicate number on a reef basisSite_rep: replicate number on a sitePCR1_binary: First technical PCR replicate: positive or negative (1 or 0)PCR2_binary: First technical PCR replicate: positive or negative (1 or 0)LOD_sample_positive: Limit of Detection based: 1 if either of the technical replicates are positivePCR1_conc: CoTS eDNA copy number per L water filtered, first technical replicatePCR2_conc: CoTS eDNA copy number per L water filtered, second technical replicateConc_mean: CoTS eDNA copy number per L water filtered, averaged over both replicatesMaintenance and Update Frequency: asNeededStatement: Documented SOP procedures include BioRad ddPCR AutoDG workflow, BioRad ddPCR AutoDG workflow and Qiacube DNA extraction of eDNA filter samples stored in 540ul ATL. 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While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.&rft_rights=Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: Australian Institute of Marine Science (AIMS). (2025). EDNA data for Crown of Thorns Seastar (Acanthaster cf. solaris) - CCIP-RT-02. https://doi.org/10.25845/rxza-nq17, accessed[date-of-access].&rft_subject=oceans&rft.type=dataset&rft.language=English Access the data
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Licence & Rights:

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Creative Commons Attribution-NonCommercial 3.0 Australia License
http://creativecommons.org/licenses/by-nc/3.0/au/

Use Limitation: All AIMS data, products and services are provided "as is" and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.

Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: "Australian Institute of Marine Science (AIMS). (2025). EDNA data for Crown of Thorns Seastar (Acanthaster cf. solaris) - CCIP-RT-02. https://doi.org/10.25845/rxza-nq17, accessed[date-of-access]".

Access:

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Full description

Following on from previous CoTS Control Innovation Program (CCIP) projects focused on developing an eDNA method to detect and quantify CoTS, the data here include field data gathered as part of an effort to operationalise previous research.


Data Dictionary
Field name: Explanation/units
Date: Julian Date
Year: Collection Year
Reef: Reef Name
ReefName: Official GBRMPA Reef name and ID
Site_name: Site name
Site_No: Site number
Lat: Latitude in decimal degrees
Long: Longitude in decimal degrees
Project: Project Code
Collection organisation: Organisation that conducted the primary collection
Collection vessel: Vessel from which samples were collected
Reef_rep: replicate number on a reef basis
Site_rep: replicate number on a site
PCR1_binary: First technical PCR replicate: positive or negative (1 or 0)
PCR2_binary: First technical PCR replicate: positive or negative (1 or 0)
LOD_sample_positive: Limit of Detection based: 1 if either of the technical replicates are positive
PCR1_conc: CoTS eDNA copy number per L water filtered, first technical replicate
PCR2_conc: CoTS eDNA copy number per L water filtered, second technical replicate
Conc_mean: CoTS eDNA copy number per L water filtered, averaged over both replicates

Lineage

Maintenance and Update Frequency: asNeeded
Statement: Documented SOP procedures include BioRad ddPCR AutoDG workflow, BioRad ddPCR AutoDG workflow and Qiacube DNA extraction of eDNA filter samples stored in 540ul ATL. Excel spreadsheet converted to .csv before upload to data repository.

Notes

Credit
Gomez Cabrera, M. (AIMS)
Credit
Uthicke, S. (AIMS)
Credit
Doyle, J. (AIMS)
Credit
CoTS Control Innovation Program (CCIP)
Credit
Australian Institute of Marine Science (AIMS), Australia.

Modified: 18 09 2026

This dataset is part of a larger collection

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Subjects
oceans |

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Other Information
Reef Geohub

url : https://reef-geohub-ext-geohubgbrmpa.hub.arcgis.com/datasets/816f4d747cd94d84acfd924b27f581d8_64/explore

Previous research: EDNA data for Crown of Thorns Seastar (Acanthaster cf. solaris)

doi : https://doi.org/10.25845/2XC1-XJ49

Previous research: Developing an effective marine eDNA monitoring: eDNA detection at pre-outbreak densities of corallivorous seastar (Acanthaster cf. solaris)

url : https://apps.aims.gov.au/metadata/view/d2963d59-f6a2-4241-bd1d-6cf3873776f1

global : 7b778538-3631-466e-ad77-d87678e1b862

Identifiers
  • global : bfcf0568-adca-4810-8c5e-56a12ea48a43
ACN 633 798 857