Data

EDNA data for Crown of Thorns Seastar (Acanthaster cf. solaris) - CCIP-D-03

Australian Institute of Marine Science
Australian Institute of Marine Science (AIMS)
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
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Data Dictionary:Field name: Explanation/unitsDate: Julian DateYear: Collection YearReef: GBR Reef NameSite_name: Site nameSite_No: Site numberLat: Latitude in decimal degreesLong: Longitude in decimal degreesProject: Project CodeReef_rep: replicate number on a reef basisSite_rep: replicate number on a sitePCR1_binary: First technical PCR replicate: positive or negative (1 or 0)PCR2_binary: First technical PCR replicate: positive or negative (1 or 0)LOD_sample_positive: Limit of Detection based: 1 if either of the technical replicates are positivePCR1_conc: CoTS eDNA copy number per L water filtered, first technical replicatePCR2_conc: CoTS eDNA copy number per L water filtered, second technical replicateConc_mean: CoTS eDNA copy number per L water filtered, averaged over both replicatesMaintenance and Update Frequency: asNeededStatement: Documented SOP procedures include BioRad ddPCR AutoDG workflow, BioRad ddPCR AutoDG workflow and Qiacube DNA extraction of eDNA filter samples stored in 540ul ATL.&rft.creator=Australian Institute of Marine Science (AIMS) &rft.date=2026&rft.coverage=westlimit=145.4851; 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While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.&rft_rights=Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: Australian Institute of Marine Science (AIMS). (2025). EDNA data for Crown of Thorns Seastar (Acanthaster cf. solaris) - CCIP-D-03. https://doi.org/10.25845/2XC1-XJ49, accessed[date-of-access].&rft_subject=oceans&rft.type=dataset&rft.language=English Access the data
Cite Saved to MyRDA Save to MyRDA

Licence & Rights:

Other view details
Unknown

Creative Commons Attribution-NonCommercial 3.0 Australia License
http://creativecommons.org/licenses/by-nc/3.0/au/

Use Limitation: All AIMS data, products and services are provided "as is" and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.

Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: "Australian Institute of Marine Science (AIMS). (2025). EDNA data for Crown of Thorns Seastar (Acanthaster cf. solaris) - CCIP-D-03. https://doi.org/10.25845/2XC1-XJ49, accessed[date-of-access]".

Access:

Other

Full description

AIMS developed an eDNA based methods to detect and quantify CoTS. The data included here include field data from initial method development as part of AIMS appropriation, and later testing through several CoTS Control Innovation Program (CCIP) projects. Data consist of filtered water samples, in most cases collected with 12 replicates at three sites per reef.


Data Dictionary:


Field name: Explanation/units


Date: Julian Date


Year: Collection Year


Reef: GBR Reef Name


Site_name: Site name


Site_No: Site number


Lat: Latitude in decimal degrees


Long: Longitude in decimal degrees


Project: Project Code


Reef_rep: replicate number on a reef basis


Site_rep: replicate number on a site


PCR1_binary: First technical PCR replicate: positive or negative (1 or 0)


PCR2_binary: First technical PCR replicate: positive or negative (1 or 0)


LOD_sample_positive: Limit of Detection based: 1 if either of the technical replicates are positive


PCR1_conc: CoTS eDNA copy number per L water filtered, first technical replicate


PCR2_conc: CoTS eDNA copy number per L water filtered, second technical replicate


Conc_mean: CoTS eDNA copy number per L water filtered, averaged over both replicates

Lineage

Maintenance and Update Frequency: asNeeded
Statement: Documented SOP procedures include BioRad ddPCR AutoDG workflow, BioRad ddPCR AutoDG workflow and Qiacube DNA extraction of eDNA filter samples stored in 540ul ATL.

Notes

Credit
Doyle, J. (AIMS)
Credit
Australian Institute of Marine Science (AIMS), Australia.
Credit
Uthicke, S. (AIMS)
Credit
Gomez Cabrera, M. (AIMS)
Credit
CoTS Control Innovation Program (CCIP)

Modified: 11 09 2026

This dataset is part of a larger collection

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Subjects
oceans |

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Other Information
Qiacube DNA extraction of eDNA filter samples stored in 540ul ATL [PDFsize: 166 KB]

url : https://api.aims.gov.au/data-v2.0/1d769b2d-4ee9-47ca-baec-90b2d9fe0b9a/files/Qiacube DNA extraction of eDNA filter samples stored in 540ul ATL.pdf

Uthicke, S., Gomez Cabrera, M., Patel, F., Lawerence, E., Tabor, B., Foster, S., & Doyle, J. (2025). Operationalising and implementing crown-of-thorns starfish (COTS) environmental (eDNA) monitoring on the Great Barrier Reef. A report to the Australian Government by the COTS Control Innovation Program (97 pp). https://www.barrierreef.org/uploads/CCIP-D-03-Final-Report-eDNA-Monitoring.pdf

url : https://www.barrierreef.org/uploads/CCIP-D-03-Final-Report-eDNA-Monitoring.pdf

CoTS eDNA sampling with standard filter housings [PDF size: 753 KB]

url : https://api.aims.gov.au/data-v2.0/1d769b2d-4ee9-47ca-baec-90b2d9fe0b9a/files/CoTS eDNA sampling with standard filter housings.pdf

Uthicke, S., Doyle, J. R., Gomez Cabrera, M., Patel, F., McLatchie, M. J., Doll, P. C., Chandler, J. F., & Pratchett, M. S. (2024). eDNA monitoring detects new outbreak wave of corallivorous seastar (Acanthaster cf. solaris) at Lizard Island, Great Barrier Reef. Coral Reefs, 43, 857-866. https://doi.org/10.1007/s00338-024-02506-8

doi : https://doi.org/10.1007/s00338-024-02506-8

Associated metadata record for Uthicke et al (2022)

url : https://apps.aims.gov.au/metadata/view/d2963d59-f6a2-4241-bd1d-6cf3873776f1

Uthicke, S., Robson, B., Doyle, J. R., Logan, M., Pratchett, M. S., & Lamare, M. (2022). Developing an effective marine eDNA monitoring: EDNA detection at pre-outbreak densities of corallivorous seastar (Acanthaster cf. Solaris). Science of The Total Environment, 851, 158143. https://doi.org/10.1016/j.scitotenv.2022.158143

doi : https://doi.org/10.1016/j.scitotenv.2022.158143

BioRad ddPCR AutoDG workflow [PDF size: 400 KB]

url : https://api.aims.gov.au/data-v2.0/1d769b2d-4ee9-47ca-baec-90b2d9fe0b9a/files/BioRad ddPCR AutoDG workflow.pdf

global : 7b778538-3631-466e-ad77-d87678e1b862

Identifiers
  • global : 1d769b2d-4ee9-47ca-baec-90b2d9fe0b9a
ACN 633 798 857