Data

Distribution of Vitamin B12 in waters of the central Great Barrier Reef

Australian Institute of Marine Science
Australian Institute of Marine Science (AIMS)
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=https://apps.aims.gov.au/metadata/view/827e2ae3-27b4-4e07-96e9-745fca76101b&rft.title=Distribution of Vitamin B12 in waters of the central Great Barrier Reef&rft.identifier=https://apps.aims.gov.au/metadata/view/827e2ae3-27b4-4e07-96e9-745fca76101b&rft.publisher=Australian Institute of Marine Science (AIMS)&rft.description=A highly sensitive and selective method was used to estimate concentrations of cyanocobalamin (Vitamin B12) in sea water along a transect of reefs across the central portion of the Great Barrier Reef. Water samples were collected from a depth of 0.5 m along each transect (moving from reef front to reef back) at Pandora, Rib and Myrmidon Reefs. Samples were also collected from Palm Passage at a depth of 10m. Analyses were based on clinical techniques of radioimmunoassay, combining the RIA method with preconcentration of Vitamin B12 by displacement liquid chromatography on C18 SEP-PAK columns. Analyses of the column eluate by radioassay proved sensitive to B12 at femtomolar concentrations (50-2400 pg B12/L seawater). The concentrations calculated from the radioassay translated directly into units of pg B12/L and ng folate/L seawater as a consequence of the concentration procedure. Concentrations above calibration range (2400 pg B 12; 24.0 ng folate) were determined by extrapolation. To determine changes in Vitamin B12 concentrations of sea water along a transect of reefs across the central portion of the Great Barrier Reef.Maintenance and Update Frequency: notPlannedStatement: Statement: Care was taken to avoid surfactant material at the sea-surface interface when collecting samples.&rft.creator=Australian Institute of Marine Science (AIMS) &rft.date=2024&rft.coverage=westlimit=147.389124; southlimit=-18.266752; eastlimit=147.389124; northlimit=-18.266752&rft.coverage=westlimit=147.389124; southlimit=-18.266752; eastlimit=147.389124; northlimit=-18.266752&rft.coverage=westlimit=146.429285; southlimit=-18.81339; eastlimit=146.429285; northlimit=-18.81339&rft.coverage=westlimit=146.429285; southlimit=-18.81339; eastlimit=146.429285; northlimit=-18.81339&rft.coverage=westlimit=146.870381; southlimit=-18.479293; eastlimit=146.870381; northlimit=-18.479293&rft.coverage=westlimit=146.870381; southlimit=-18.479293; eastlimit=146.870381; northlimit=-18.479293&rft.coverage=westlimit=147.07; southlimit=-18.325; eastlimit=147.07; northlimit=-18.325&rft.coverage=westlimit=147.07; southlimit=-18.325; eastlimit=147.07; northlimit=-18.325&rft.coverage=westlimit=147.198333; southlimit=-18.208333; eastlimit=147.198333; northlimit=-18.208333&rft.coverage=westlimit=147.198333; southlimit=-18.208333; eastlimit=147.198333; northlimit=-18.208333&rft.coverage=westlimit=146.851667; southlimit=-18.463333; eastlimit=146.851667; northlimit=-18.463333&rft.coverage=westlimit=146.851667; southlimit=-18.463333; eastlimit=146.851667; northlimit=-18.463333&rft_rights=Creative Commons Attribution-NonCommercial 3.0 Australia License http://creativecommons.org/licenses/by-nc/3.0/au/&rft_rights=Use Limitation: All AIMS data, products and services are provided as is and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.&rft_rights=Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: Australian Institute of Marine Science (AIMS). (2009). Distribution of Vitamin B12 in waters of the central Great Barrier Reef. https://apps.aims.gov.au/metadata/view/827e2ae3-27b4-4e07-96e9-745fca76101b, accessed[date-of-access].&rft_rights=Resource Usage:Use of the AIMS data is for not-for-profit applications only. All other users shall seek permission for use by contacting AIMS. Acknowledgements as prescribed must be clearly set out in the user's formal communications or publications.&rft_subject=oceans&rft.type=dataset&rft.language=English Access the data
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Creative Commons Attribution-NonCommercial 3.0 Australia License
http://creativecommons.org/licenses/by-nc/3.0/au/

Use Limitation: All AIMS data, products and services are provided "as is" and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.

Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: "Australian Institute of Marine Science (AIMS). (2009). Distribution of Vitamin B12 in waters of the central Great Barrier Reef. https://apps.aims.gov.au/metadata/view/827e2ae3-27b4-4e07-96e9-745fca76101b, accessed[date-of-access]".

Resource Usage:Use of the AIMS data is for not-for-profit applications only. All other users shall seek permission for use by contacting AIMS. Acknowledgements as prescribed must be clearly set out in the user's formal communications or publications.

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Brief description

A highly sensitive and selective method was used to estimate concentrations of cyanocobalamin (Vitamin B12) in sea water along a transect of reefs across the central portion of the Great Barrier Reef. Water samples were collected from a depth of 0.5 m along each transect (moving from reef front to reef back) at Pandora, Rib and Myrmidon Reefs. Samples were also collected from Palm Passage at a depth of 10m. Analyses were based on clinical techniques of radioimmunoassay, combining the RIA method with preconcentration of Vitamin B12 by displacement liquid chromatography on C18 SEP-PAK columns. Analyses of the column eluate by radioassay proved sensitive to B12 at femtomolar concentrations (50-2400 pg B12/L seawater). The concentrations calculated from the radioassay translated directly into units of pg B12/L and ng folate/L seawater as a consequence of the concentration procedure. Concentrations above calibration range (2400 pg B 12; 24.0 ng folate) were determined by extrapolation. To determine changes in Vitamin B12 concentrations of sea water along a transect of reefs across the central portion of the Great Barrier Reef.

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Maintenance and Update Frequency: notPlanned
Statement: Statement: Care was taken to avoid surfactant material at the sea-surface interface when collecting samples.

Notes

Credit
Dunlap, Walter C, Dr (Principal Investigator)

Modified: 17 10 2024

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147.38912,-18.26675

147.389124,-18.266752

146.42929,-18.81339

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146.87038,-18.47929

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147.07,-18.325

147.07,-18.325

147.19833,-18.20833

147.198333,-18.208333

146.85167,-18.46333

146.851667,-18.463333

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text: westlimit=147.07; southlimit=-18.325; eastlimit=147.07; northlimit=-18.325

text: westlimit=147.198333; southlimit=-18.208333; eastlimit=147.198333; northlimit=-18.208333

text: westlimit=146.851667; southlimit=-18.463333; eastlimit=146.851667; northlimit=-18.463333

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Other Information
Distribution of Vitamin B12 in reef waters of the Central Great Barrier Reef: Dunlap WC (1983) Distribution of Vitamin B12 in reef waters of the Central Great Barrier Reef. pp. 305-309. In: Baker JT, Carter RM, Sammarco PW and Stark KP (eds) Proceedings: Inaugural Great Barrier Reef Conference, Townsville Aug 28 - Sept 2, 1983. James Cook University Press.

local : articleId=2982

Identifiers
  • global : 827e2ae3-27b4-4e07-96e9-745fca76101b