Data

Developing an effective marine eDNA monitoring: eDNA detection at pre-outbreak densities of corallivorous seastar (Acanthaster cf. solaris)

Name: Developing an effective marine eDNA monitoring: eDNA detection at pre-outbreak densities of corallivorous seastar (Acanthaster cf. solaris)
Published: 2026

Australian Institute of Marine Science

Australian Institute of Marine Science (AIMS)

Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]

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Reefs locations were: Lizard Island Lodestone Reef Davies Reef Elizabeth ReefSample collections occurred two to three times per site over a few days.Underwater transect (50 m × 4 m ) surveys were conducted once per trip between 3 – 6 transects. Underwater surveys were complemented with 30 2-minute manta tows in 2018 (Elizabeth Reef). Long Term Monitoring data were collected for Lizard Island Reefs, with between 80 and 90 transects conducted in years 2017, 2018 and 2021. Scooter-assisted large area diver-based visual surveys at Lizard Island were conducted at Lizard Island in 2019 and 2020.DNA extraction was prepared for water sample analysis, using digital droplet PCR analysis (ddPCR).eReefs RECOM was used to extract values of water residence age (age of water over reefs), temperature and tide level (sea surface elevation) for each site at each sampling time.Statistical analyses were conducted using generalized linear mixed models to test for the effects of eReefs derived parameters (water residence age, surface elevation and temperature) and CoTS density at the site x visit level on the number of CoTS eDNA copies per lL of water.Maintenance and Update Frequency: asNeeded&rft.creator=Australian Institute of Marine Science (AIMS) &rft.date=2026&rft.coverage=westlimit=145.455; southlimit=-14.6875; eastlimit=145.455; northlimit=-14.6875&rft.coverage=westlimit=145.455; southlimit=-14.6875; eastlimit=145.455; northlimit=-14.6875&rft.coverage=westlimit=145.4536; southlimit=-14.646; eastlimit=145.4536; northlimit=-14.646&rft.coverage=westlimit=145.4536; southlimit=-14.646; eastlimit=145.4536; northlimit=-14.646&rft.coverage=westlimit=145.4489; southlimit=-14.6616; eastlimit=145.4489; northlimit=-14.6616&rft.coverage=westlimit=145.4489; southlimit=-14.6616; eastlimit=145.4489; northlimit=-14.6616&rft.coverage=westlimit=145.4409; southlimit=-14.6744; eastlimit=145.4409; northlimit=-14.6744&rft.coverage=westlimit=145.4409; southlimit=-14.6744; eastlimit=145.4409; northlimit=-14.6744&rft.coverage=westlimit=145.4394; southlimit=-14.6838; eastlimit=145.4394; northlimit=-14.6838&rft.coverage=westlimit=145.4394; southlimit=-14.6838; eastlimit=145.4394; northlimit=-14.6838&rft.coverage=westlimit=147.1; southlimit=-18.6881; eastlimit=147.1; northlimit=-18.6881&rft.coverage=westlimit=147.1; southlimit=-18.6881; eastlimit=147.1; northlimit=-18.6881&rft.coverage=westlimit=147.1093; southlimit=-18.6999; eastlimit=147.1093; northlimit=-18.6999&rft.coverage=westlimit=147.1093; southlimit=-18.6999; eastlimit=147.1093; northlimit=-18.6999&rft.coverage=westlimit=147.0956; southlimit=-18.689; eastlimit=147.0956; northlimit=-18.689&rft.coverage=westlimit=147.0956; southlimit=-18.689; eastlimit=147.0956; northlimit=-18.689&rft.coverage=westlimit=147.6294; southlimit=-18.8263; eastlimit=147.6294; northlimit=-18.8263&rft.coverage=westlimit=147.6294; southlimit=-18.8263; eastlimit=147.6294; northlimit=-18.8263&rft.coverage=westlimit=147.6355; southlimit=-18.8309; eastlimit=147.6355; northlimit=-18.8309&rft.coverage=westlimit=147.6355; southlimit=-18.8309; eastlimit=147.6355; northlimit=-18.8309&rft.coverage=westlimit=147.9263; southlimit=-19.0381; eastlimit=147.9263; northlimit=-19.0381&rft.coverage=westlimit=147.9263; southlimit=-19.0381; eastlimit=147.9263; northlimit=-19.0381&rft.coverage=westlimit=147.9363; southlimit=-19.0308; eastlimit=147.9363; northlimit=-19.0308&rft.coverage=westlimit=147.9363; southlimit=-19.0308; eastlimit=147.9363; northlimit=-19.0308&rft.coverage=westlimit=149.0536; southlimit=-19.3294; eastlimit=149.0536; northlimit=-19.3294&rft.coverage=westlimit=149.0536; southlimit=-19.3294; eastlimit=149.0536; northlimit=-19.3294&rft.coverage=westlimit=149.0505; southlimit=-19.3343; eastlimit=149.0505; northlimit=-19.3343&rft.coverage=westlimit=149.0505; southlimit=-19.3343; eastlimit=149.0505; northlimit=-19.3343&rft_rights=Creative Commons Attribution 3.0 Australia License http://creativecommons.org/licenses/by/3.0/au/&rft_rights=Use Limitation: All AIMS data, products and services are provided as is and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.&rft_rights=Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: Australian Institute of Marine Science (AIMS). (2022). Developing an effective marine eDNA monitoring: eDNA detection at pre-outbreak densities of corallivorous seastar (Acanthaster cf. solaris). https://apps.aims.gov.au/metadata/view/d2963d59-f6a2-4241-bd1d-6cf3873776f1, accessed[date-of-access].&rft_subject=oceans&rft.type=dataset&rft.language=English Access the data

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Use Limitation: All AIMS data, products and services are provided "as is" and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.

Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: "Australian Institute of Marine Science (AIMS). (2022). Developing an effective marine eDNA monitoring: eDNA detection at pre-outbreak densities of corallivorous seastar (Acanthaster cf. solaris). https://apps.aims.gov.au/metadata/view/d2963d59-f6a2-4241-bd1d-6cf3873776f1, accessed[date-of-access]".

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Full description

This study aimed to understand if physical environmental variables in¿uence eDNA measurements evaluating three key variables associated with reef ¿ushing, tidal exchange and sea temperature, and their impact to improve prediction of eDNA concentration.

Water samples for eDNA analysis were collected from four mid-shelf reefs in the Central section of the GBR and from one reef in the Northern section of the GBR. Samples were collected over three consecutive years from 2018 – 2020. Reefs locations were:

    Lizard Island

    Lodestone Reef

    Davies Reef

    Elizabeth Reef

Sample collections occurred two to three times per site over a few days.

Underwater transect (50 m × 4 m ) surveys were conducted once per trip between 3 – 6 transects. Underwater surveys were complemented with 30 2-minute manta tows in 2018 (Elizabeth Reef). Long Term Monitoring data were collected for Lizard Island Reefs, with between 80 and 90 transects conducted in years 2017, 2018 and 2021. Scooter-assisted large area diver-based visual surveys at Lizard Island were conducted at Lizard Island in 2019 and 2020.

DNA extraction was prepared for water sample analysis, using digital droplet PCR analysis (ddPCR).

eReefs RECOM was used to extract values of water residence age (age of water over reefs), temperature and tide level (sea surface elevation) for each site at each sampling time.

Statistical analyses were conducted using generalized linear mixed models to test for the effects of eReefs derived parameters (water residence age, surface elevation and temperature) and CoTS density at the site x visit level on the number of CoTS eDNA copies per lL of water.

Lineage

Maintenance and Update Frequency: asNeeded

Modified: 18 09 2026

This dataset is part of a larger collection

Click to explore relationships graph

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Subjects

oceans |

User Contributed Tags

Other Information

Uthicke, S., Robson, B., Doyle, J. R., Logan, M., Pratchett, M. S., & Lamare, M. (2022). Developing an effective marine eDNA monitoring: EDNA detection at pre-outbreak densities of corallivorous seastar (Acanthaster cf. Solaris). Science of The Total Environment, 851, 158143. https://doi.org/10.1016/j.scitotenv.2022.158143

doi : https://doi.org/10.1016/j.scitotenv.2022.158143 DOI Link

GitHub eReefs package - https://github.com/open-AIMS/ereefs

url : https://github.com/open-AIMS/ereefs External Link

Identifiers

global : d2963d59-f6a2-4241-bd1d-6cf3873776f1