Data

Decellularization of porcine liver tissues

Queensland University of Technology
Ravichandran, Akhilandeshwari ; Murekatete, Berline ; Moedder, Denise ; Meinert, Christoph ; Bray, Laura
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.25912/RDF_1636688100143&rft.title=Decellularization of porcine liver tissues&rft.identifier=10.25912/RDF_1636688100143&rft.publisher=Queensland University of Technology&rft.description=Porcine liver tissues were decellularized to obtain extracellular matrix using chemical detergent based protocols (Protocol I and Protocol II) over a period of 5 days. The extent of decellularization was quantified by assessing the DNA content remaining in the tissues after each day of the chemical treatment using the Picogreen double strand DNA quantification kit. This was normalized to the DNA content of the raw liver tissue to calculate the efficacy of the protocols to remove cellular material from the tissues. &rft.creator=Ravichandran, Akhilandeshwari &rft.creator=Murekatete, Berline &rft.creator=Moedder, Denise &rft.creator=Meinert, Christoph &rft.creator=Bray, Laura &rft.date=2021&rft.edition=1&rft.coverage=153.014003,-27.452963&rft_rights=© Queensland University of Technology, 2021. &rft_rights=Creative Commons Attribution 3.0 http://creativecommons.org/licenses/by/4.0/&rft_subject=Decellularization&rft_subject=ECM Hydrogels&rft_subject=Liver tissue engineering&rft.type=dataset&rft.language=English Access the data
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Creative Commons Attribution 3.0
http://creativecommons.org/licenses/by/4.0/

© Queensland University of Technology, 2021.

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Contact Information

Postal Address:
Dr Akhilandeshwari Ravichandran
Ph: +61 469 026 385

akhilandeshwari.ravichandran@qut.edu.au

Full description

Porcine liver tissues were decellularized to obtain extracellular matrix using chemical detergent based protocols (Protocol I and Protocol II) over a period of 5 days. The extent of decellularization was quantified by assessing the DNA content remaining in the tissues after each day of the chemical treatment using the Picogreen double strand DNA quantification kit. This was normalized to the DNA content of the raw liver tissue to calculate the efficacy of the protocols to remove cellular material from the tissues.

Data time period: 23 01 2020 to 21 10 2020

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153.014,-27.45296

153.014003,-27.452963

Subjects
Decellularization | ECM Hydrogels | Liver tissue engineering |

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