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Data: confocal reflectance imaging as a highly sensitive imaging modality for 3D cell culture

The University of Queensland
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.48610/7f3ae88&rft.title=Data: confocal reflectance imaging as a highly sensitive imaging modality for 3D cell culture&rft.identifier=RDM ID: 5f9190b9-5fc6-4363-8a2e-298de5ba8397&rft.publisher=The University of Queensland&rft.description=Data used to demonstrate confocal reflectance imaging as a highly sensitive imaging modality for 3D cell culture. Data includes reflectance and fluorescence imaging of cultured osteocyte and fibroblast cells in 3D polyisocyanopeptide (PIC) hydrogel, as well as collagen, fibrin, and Matrigel. Confocal reflectance imaging typically suffers high background and poor sensitivity. We demonstrate sensitive and low-background reflectance imaging of cells encapsulated in transparent 3D hydrogels. Nanoscale cell morphology is visualized with sensitivity similar to confocal fluorescence, with low laser power, minimal specimen preparation, and reduced toxicity.&rft.creator=Anonymous&rft.date=2024&rft_rights= https://guides.library.uq.edu.au/deposit-your-data/license-reuse-data-agreement&rft_subject=eng&rft_subject=Classical and Physical Optics&rft_subject=PHYSICAL SCIENCES&rft_subject=OPTICAL PHYSICS&rft_subject=Biochemistry and Cell Biology not elsewhere classified&rft_subject=BIOLOGICAL SCIENCES&rft_subject=BIOCHEMISTRY AND CELL BIOLOGY&rft.type=dataset&rft.language=English Access the data
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[email protected]
Australian Institute for Bioengineering and Nanotechnology

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Data used to demonstrate confocal reflectance imaging as a highly sensitive imaging modality for 3D cell culture. Data includes reflectance and fluorescence imaging of cultured osteocyte and fibroblast cells in 3D polyisocyanopeptide (PIC) hydrogel, as well as collagen, fibrin, and Matrigel. Confocal reflectance imaging typically suffers high background and poor sensitivity. We demonstrate sensitive and low-background reflectance imaging of cells encapsulated in transparent 3D hydrogels. Nanoscale cell morphology is visualized with sensitivity similar to confocal fluorescence, with low laser power, minimal specimen preparation, and reduced toxicity.

Issued: 2024

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Subjects
Biochemistry and Cell Biology | Biological Sciences | Biochemistry and Cell Biology Not Elsewhere Classified | Classical and Physical Optics | Optical Physics | Physical Sciences | eng |

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ACN 633 798 857