Research Project
Full description This study aimed to develop a new technique using MR microimaging at ultra-high field, without contrast agents, to visualise glomeruli in human nephrectomy samples. MRI was performed at 16.4 T using three-dimensional T1/T2*-weighted gradient echo imaging at 30 um isotropic resolution on cortical samples. A segmentation pipeline based on shape recognition and clustering was developed to count glomeruli on the 30 um MR images. Glomerular number and density were estimated after MRI using unbiased stereology, and their distribution were visualised using a subset of samples imaged using confocal microscopy