Data

Clone library using primers for gammaproteobacteria from an SAB treatment in the SRE4 experiment

Australian Antarctic Data Centre
BOWMAN, JOHN ; POWELL, SHANE
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.26179/5d4baa1262fa2&rft.title=Clone library using primers for gammaproteobacteria from an SAB treatment in the SRE4 experiment&rft.identifier=10.26179/5d4baa1262fa2&rft.publisher=Australian Antarctic Data Centre&rft.description=A clone library was created from DNA extracted from an SAB-treated sample from the SRE4 in situ biodegradation experiment. The clone libary was created using one universal primer and one primer designed to be specific for the gammaproteobacteria. Sequences of approximately 600 bp were obtained. The samples used in this experiment were collected from O'Brien Bay, near Casey Station in the Windmill Islands. Gammaproteobacteria clone library Clone library created from SRE4 T2 SAB sample using primers 10F (GAG TTT GAT CCT GGC TCA G ) and GAMR (GGT AAG GTT CTT CGC GTT GCA T). Clones sequenced on a CEQ8000 Genetic Analysis system (Beckman-Coulter) and alignments were done in BioEdit v 5.0.9. Text file SRE4gammaclonesalign is a text version of BioEdit file SRE4gammaclones. This work was completed as part of ASAC project 2672 (ASAC_2672).&rft.creator=BOWMAN, JOHN &rft.creator=POWELL, SHANE &rft.date=2006&rft.coverage=northlimit=-66; southlimit=-66; westlimit=110; eastLimit=110; projection=WGS84&rft.coverage=northlimit=-66; southlimit=-66; westlimit=110; eastLimit=110; projection=WGS84&rft_rights=This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=SRE4_SAB_gammaclones when using these data.&rft_subject=environment&rft_subject=OIL SPILLS&rft_subject=EARTH SCIENCE&rft_subject=HUMAN DIMENSIONS&rft_subject=ENVIRONMENTAL IMPACTS&rft_subject=CLONE&rft_subject=SPECIAL ANTARCTIC BLEND DIESEL&rft_subject=SAB&rft_subject=ADS > Automated DNA Sequencer&rft_subject=LABORATORY&rft_subject=FIELD INVESTIGATION&rft_subject=CONTINENT > ANTARCTICA > Windmill Islands&rft_subject=GEOGRAPHIC REGION > POLAR&rft_place=Hobart&rft.type=dataset&rft.language=English Access the data
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This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=SRE4_SAB_gammaclones when using these data.

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Brief description

A clone library was created from DNA extracted from an SAB-treated sample from the SRE4 in situ biodegradation experiment. The clone libary was created using one universal primer and one primer designed to be specific for the gammaproteobacteria. Sequences of approximately 600 bp were obtained. The samples used in this experiment were collected from O'Brien Bay, near Casey Station in the Windmill Islands. Gammaproteobacteria clone library Clone library created from SRE4 T2 SAB sample using primers 10F (GAG TTT GAT CCT GGC TCA G ) and GAMR (GGT AAG GTT CTT CGC GTT GCA T). Clones sequenced on a CEQ8000 Genetic Analysis system (Beckman-Coulter) and alignments were done in BioEdit v 5.0.9. Text file SRE4gammaclonesalign is a text version of BioEdit file SRE4gammaclones. This work was completed as part of ASAC project 2672 (ASAC_2672).

Issued: 2006-04-06

Data time period: 2002-12-01 to 2002-12-31

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110,-66

110,-66

text: northlimit=-66; southlimit=-66; westlimit=110; eastLimit=110; projection=WGS84

Subjects
ADS > Automated DNA Sequencer | CLONE | CONTINENT > ANTARCTICA > Windmill Islands | EARTH SCIENCE | ENVIRONMENTAL IMPACTS | FIELD INVESTIGATION | GEOGRAPHIC REGION > POLAR | HUMAN DIMENSIONS | LABORATORY | OIL SPILLS | SAB | SPECIAL ANTARCTIC BLEND DIESEL | environment |

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AU-ANL:PEAU : http://nla.gov.au/nla.party-617536

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