Data

Real-time PCR measurements of alkB and rpoB in SAB contaminated sediment (SRE4 experiment).

Australian Antarctic Division
Bowman, J. and Powell, S. ; BOWMAN, JOHN ; POWELL, SHANE
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=http://data.aad.gov.au/metadata/SRE4_alkB_T1T2&rft.title=Real-time PCR measurements of alkB and rpoB in SAB contaminated sediment (SRE4 experiment).&rft.identifier=http://data.aad.gov.au/metadata/SRE4_alkB_T1T2&rft.publisher=Australian Antarctic Data Centre&rft.description=Real-time PCR assays were used to measure the proportion of the microbial population that had the potential to degrade alkanes - the principal component of Special Antarctic Blend diesel. This was done by developing assays to measure the levels of alkane mono-oxygenase genes (alkB) relative to ribosomal polymerase B sub-unit (rpoB) genes in soil and sediment samples. In this work, the assays were used to determine the levels of these genes in sediment exposed to SAB and control sediments from the SRE4 in-situ biodegradation experiment.The samples used in this experiment were collected from O'Brien Bay, near Casey Station in the Windmill Islands.An information sheet is provided at the beginning of the excel workbook.This work was completed as part of ASAC project 2672 (ASAC_2672).Progress Code: completedStatement: The dates provided in temporal coverage are approximate only. Year and month are correct.&rft.creator=Bowman, J. and Powell, S. &rft.creator=BOWMAN, JOHN &rft.creator=POWELL, SHANE &rft.date=2006&rft.coverage=westlimit=110.23682; southlimit=-66.55045; eastlimit=110.68176; northlimit=-66.21152&rft.coverage=westlimit=110.23682; southlimit=-66.55045; eastlimit=110.68176; northlimit=-66.21152&rft_rights=These data are publicly available for download from the provided URL.&rft_rights=Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/legalcode&rft_rights=This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=SRE4_alkB_T1T2 when using these data.&rft_rights=This metadata record is publicly available.&rft_subject=environment&rft_subject=EARTH SCIENCE > HUMAN DIMENSIONS > ENVIRONMENTAL IMPACTS > OIL SPILLS&rft_subject=ALKANE MONO-OXYGENASE&rft_subject=PCR&rft_subject=SAB&rft_subject=REAL-TIME QUANTITATIVE PCR&rft_subject=RPOB&rft_subject=SPECIAL ANTARCTIC BLEND DIESEL&rft_subject=NOT APPLICABLE&rft_subject=LABORATORY&rft_subject=FIELD INVESTIGATION&rft_subject=AMD/AU&rft_subject=CEOS&rft_subject=AMD&rft_subject=CONTINENT > ANTARCTICA > Windmill Islands&rft_subject=GEOGRAPHIC REGION > POLAR&rft.type=dataset&rft.language=English Access the data
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Attribution 4.0 International (CC BY 4.0)
https://creativecommons.org/licenses/by/4.0/legalcode

These data are publicly available for download from the provided URL.

This data set conforms to the CCBY Attribution License
(http://creativecommons.org/licenses/by/4.0/).

Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=SRE4_alkB_T1T2 when using these data.

This metadata record is publicly available.

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Full description

Real-time PCR assays were used to measure the proportion of the microbial population that had the potential to degrade alkanes - the principal component of Special Antarctic Blend diesel. This was done by developing assays to measure the levels of alkane mono-oxygenase genes (alkB) relative to ribosomal polymerase B sub-unit (rpoB) genes in soil and sediment samples. In this work, the assays were used to determine the levels of these genes in sediment exposed to SAB and control sediments from the SRE4 in-situ biodegradation experiment.

The samples used in this experiment were collected from O'Brien Bay, near Casey Station in the Windmill Islands.

An information sheet is provided at the beginning of the excel workbook.

This work was completed as part of ASAC project 2672 (ASAC_2672).

Lineage

Progress Code: completed
Statement: The dates provided in temporal coverage are approximate only. Year and month are correct.

Data time period: 2001-10-01 to 2001-10-31

Data time period: 2002-12-01 to 2002-12-31

This dataset is part of a larger collection

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110.68176,-66.21152 110.68176,-66.55045 110.23682,-66.55045 110.23682,-66.21152 110.68176,-66.21152

110.45929,-66.380985

text: westlimit=110.23682; southlimit=-66.55045; eastlimit=110.68176; northlimit=-66.21152

Subjects
ALKANE MONO-OXYGENASE | AMD | AMD/AU | CEOS | CONTINENT > ANTARCTICA > Windmill Islands | EARTH SCIENCE > HUMAN DIMENSIONS > ENVIRONMENTAL IMPACTS > OIL SPILLS | FIELD INVESTIGATION | GEOGRAPHIC REGION > POLAR | LABORATORY | NOT APPLICABLE | PCR | REAL-TIME QUANTITATIVE PCR | RPOB | SAB | SPECIAL ANTARCTIC BLEND DIESEL | environment |

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Other Information
Download point for the data. (GET DATA)

uri : https://data.aad.gov.au/eds/1464/download

Public information for ASAC project 2672 (PROJECT HOME PAGE)

uri : https://projects.aad.gov.au/search_projects_results.cfm?project_no=2672

Citation reference for this metadata record and dataset (VIEW RELATED INFORMATION)

uri : http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=SRE4_alkB_T1T2

Identifiers
  • global : SRE4_alkB_T1T2