Data

Real-time PCR measurements of alkB and rpoB in SAB contaminated sediment (SRE4 experiment).

Australian Antarctic Data Centre
BOWMAN, JOHN ; POWELL, SHANE
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.26179/5d3f9a44c00fa&rft.title=Real-time PCR measurements of alkB and rpoB in SAB contaminated sediment (SRE4 experiment).&rft.identifier=10.26179/5d3f9a44c00fa&rft.publisher=Australian Antarctic Data Centre&rft.description=Real-time PCR assays were used to measure the proportion of the microbial population that had the potential to degrade alkanes - the principal component of Special Antarctic Blend diesel. This was done by developing assays to measure the levels of alkane mono-oxygenase genes (alkB) relative to ribosomal polymerase B sub-unit (rpoB) genes in soil and sediment samples. In this work, the assays were used to determine the levels of these genes in sediment exposed to SAB and control sediments from the SRE4 in-situ biodegradation experiment. The samples used in this experiment were collected from O'Brien Bay, near Casey Station in the Windmill Islands. An information sheet is provided at the beginning of the excel workbook. This work was completed as part of ASAC project 2672 (ASAC_2672).&rft.creator=BOWMAN, JOHN &rft.creator=POWELL, SHANE &rft.date=2006&rft.coverage=northlimit=-66.21152; southlimit=-66.55045; westlimit=110.23682; eastLimit=110.68176; projection=WGS84&rft.coverage=northlimit=-66.21152; southlimit=-66.55045; westlimit=110.23682; eastLimit=110.68176; projection=WGS84&rft_rights=This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=SRE4_alkB_T1T2 when using these data.&rft_subject=environment&rft_subject=OIL SPILLS&rft_subject=EARTH SCIENCE&rft_subject=HUMAN DIMENSIONS&rft_subject=ENVIRONMENTAL IMPACTS&rft_subject=ALKANE MONO-OXYGENASE&rft_subject=PCR&rft_subject=SAB&rft_subject=REAL-TIME QUANTITATIVE PCR&rft_subject=RPOB&rft_subject=SPECIAL ANTARCTIC BLEND DIESEL&rft_subject=NOT APPLICABLE&rft_subject=LABORATORY&rft_subject=FIELD INVESTIGATION&rft_subject=CONTINENT > ANTARCTICA > Windmill Islands&rft_subject=GEOGRAPHIC REGION > POLAR&rft_place=Hobart&rft.type=dataset&rft.language=English Access the data
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This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=SRE4_alkB_T1T2 when using these data.

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Brief description

Real-time PCR assays were used to measure the proportion of the microbial population that had the potential to degrade alkanes - the principal component of Special Antarctic Blend diesel. This was done by developing assays to measure the levels of alkane mono-oxygenase genes (alkB) relative to ribosomal polymerase B sub-unit (rpoB) genes in soil and sediment samples. In this work, the assays were used to determine the levels of these genes in sediment exposed to SAB and control sediments from the SRE4 in-situ biodegradation experiment. The samples used in this experiment were collected from O'Brien Bay, near Casey Station in the Windmill Islands. An information sheet is provided at the beginning of the excel workbook. This work was completed as part of ASAC project 2672 (ASAC_2672).

Issued: 2006-04-06

Data time period: 2001-10-01 to 2001-10-31

Data time period: 2002-12-01 to 2002-12-31

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110.68176,-66.21152 110.68176,-66.55045 110.23682,-66.55045 110.23682,-66.21152 110.68176,-66.21152

110.45929,-66.380985

text: northlimit=-66.21152; southlimit=-66.55045; westlimit=110.23682; eastLimit=110.68176; projection=WGS84

Subjects
ALKANE MONO-OXYGENASE | CONTINENT > ANTARCTICA > Windmill Islands | EARTH SCIENCE | ENVIRONMENTAL IMPACTS | FIELD INVESTIGATION | GEOGRAPHIC REGION > POLAR | HUMAN DIMENSIONS | LABORATORY | NOT APPLICABLE | OIL SPILLS | PCR | REAL-TIME QUANTITATIVE PCR | RPOB | SAB | SPECIAL ANTARCTIC BLEND DIESEL | environment |

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AU-ANL:PEAU : http://nla.gov.au/nla.party-617536

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