Data

Changes in the metabolic potential of the sponge microbiome under ocean acidification

Australian Institute of Marine Science
Australian Institute of Marine Science (AIMS)
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=https://apps.aims.gov.au/metadata/view/82fe0933-6a58-4c37-9870-77eecec0a291&rft.title=Changes in the metabolic potential of the sponge microbiome under ocean acidification&rft.identifier=https://apps.aims.gov.au/metadata/view/82fe0933-6a58-4c37-9870-77eecec0a291&rft.publisher=Australian Institute of Marine Science (AIMS)&rft.description=This study investigated the changes occurring in the functional potential of the microbiome of two marine sponge species living in low pH, acidified waters and adjacent (~ 500m away) ,control waters, at shallow coral reefs CO2 seep sites in Papua New Guinea.Field work was carried out in the Milne Bay Province in the d'Entrecasteaux Channel in Papua New Guinea at a depth of 5m. Control site GPS coordinates: 9.828217 S 150.820517 E, Seep site GPS coordinates: 9.8241 S 150.825833 E.Three samples of each of two sponge species were collected. The species were Coelocarteria singaporensis and Stylissa flabelliformis. These particular species were chosen based on a previous study (Morrow et al., 2015) characterizeing the microbial communities of the 2 species at these particular field sites.Sample size of three samples per species was chosen based on the following criteria:1) minimizing impact on the field site2) accounting for statistical testing sample size requirements3) space availability for storage in the field and during transport4) cost (temporal and financial) of sample processing and sequencingEnvironmental data were collected over multiple expeditions as well as via remote loggers. Data was collected in August-2010, January 2013 and betweek December 2011-April 2014. For more information on environmental data for this study, please see Supplementary tables 3, 3 and 4.Microbial functions of the two sponge species of the seep and control sites were compared, including transport of carbon and host derived compounds, carbon fixation and carbonic anhydrase, nitrogen and sulfur metabolism.Maintenance and Update Frequency: asNeededStatement: Sampling proceedures used in Milne Bay province of Papua New Guinea followed those described in: Morrow, K. M. et al. Natural volcanic CO2 seeps reveal future trajectories for host-microbial associations in corals and sponges. ISME J. 9, 894–908 (2015). Sampling processing and sequencing followed proceedures described in: Thomas, T. et al. Functional genomic signatures of sponge bacteria reveal unique and shared features of symbiosis. ISME J. 4, 1–11 (2010).&rft.creator=Australian Institute of Marine Science (AIMS) &rft.date=2025&rft.coverage=westlimit=150.79456329345706; southlimit=-9.852171763714034; eastlimit=150.83919525146484; northlimit=-9.820373830441108&rft.coverage=westlimit=150.79456329345706; southlimit=-9.852171763714034; eastlimit=150.83919525146484; northlimit=-9.820373830441108&rft_rights=Creative Commons Attribution 3.0 Australia License http://creativecommons.org/licenses/by/3.0/au/&rft_rights=Use Limitation: All AIMS data, products and services are provided as is and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.&rft_rights=Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: Australian Institute of Marine Science (AIMS). (2019). Changes in the metabolic potential of the sponge microbiome under ocean acidification. https://apps.aims.gov.au/metadata/view/82fe0933-6a58-4c37-9870-77eecec0a291, accessed[date-of-access].&rft_subject=oceans&rft.type=dataset&rft.language=English Access the data
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Use Limitation: All AIMS data, products and services are provided "as is" and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.

Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: "Australian Institute of Marine Science (AIMS). (2019). Changes in the metabolic potential of the sponge microbiome under ocean acidification. https://apps.aims.gov.au/metadata/view/82fe0933-6a58-4c37-9870-77eecec0a291, accessed[date-of-access]".

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This study investigated the changes occurring in the functional potential of the microbiome of two marine sponge species living in low pH, "acidified" waters and adjacent (~ 500m away) ,"control" waters, at shallow coral reefs CO2 seep sites in Papua New Guinea.


Field work was carried out in the Milne Bay Province in the d'Entrecasteaux Channel in Papua New Guinea at a depth of 5m. Control site GPS coordinates: 9.828217 S 150.820517 E, Seep site GPS coordinates: 9.8241 S 150.825833 E.


Three samples of each of two sponge species were collected. The species were Coelocarteria singaporensis and Stylissa flabelliformis. These particular species were chosen based on a previous study (Morrow et al., 2015) characterizeing the microbial communities of the 2 species at these particular field sites.


Sample size of three samples per species was chosen based on the following criteria:


1) minimizing impact on the field site
2) accounting for statistical testing sample size requirements
3) space availability for storage in the field and during transport
4) cost (temporal and financial) of sample processing and sequencing


Environmental data were collected over multiple expeditions as well as via remote loggers. Data was collected in August-2010, January 2013 and betweek December 2011-April 2014. For more information on environmental data for this study, please see Supplementary tables 3, 3 and 4.


Microbial functions of the two sponge species of the seep and control sites were compared, including transport of carbon and host derived compounds, carbon fixation and carbonic anhydrase, nitrogen and sulfur metabolism.

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Maintenance and Update Frequency: asNeeded
Statement: Sampling proceedures used in Milne Bay province of Papua New Guinea followed those described in: Morrow, K. M. et al. Natural volcanic CO2 seeps reveal future trajectories for host-microbial associations in corals and sponges. ISME J. 9, 894–908 (2015). Sampling processing and sequencing followed proceedures described in: Thomas, T. et al. Functional genomic signatures of sponge bacteria reveal unique and shared features of symbiosis. ISME J. 4, 1–11 (2010).

Notes

Credit
Wahab, M.A. A. (AIMS)
Credit
Botte, E.S. (AIMS)
Credit
Webster, N. Australian Institute of Marine Science (AIMS)

Modified: 19 09 2025

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150.8392,-9.82037 150.8392,-9.85217 150.79456,-9.85217 150.79456,-9.82037 150.8392,-9.82037

150.81687927246,-9.8362727970776

text: westlimit=150.79456329345706; southlimit=-9.852171763714034; eastlimit=150.83919525146484; northlimit=-9.820373830441108

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oceans |

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Other Information
Raw reads and MAGs are available from the NCBI SRA under accession number SRP159543.

uri : https://www.ncbi.nlm.nih.gov/sra/?term=SRP159543

Botte ES, Nielsen S, Abdul Wahab MA, Webster J, Robbins S, Thomas T, Webster NS (2019) Changes in the metabolic potential of the sponge microbiome under ocean acidification. Nature Communications 10: 4134

local : 11068/15276

Assemblies (see Supplementary Table 18 for assemblies ID) are available from IMG/MER

uri : https://img.jgi.doe.gov/cgi-bin/m/main.cgi

Source Data underlying figures in publication and supplementary figure, except fig. 11

uri : https://api.aims.gov.au/data-v2.0/82fe0933-6a58-4c37-9870-77eecec0a291/files/Source data-Botte et.al.xlsx

Supplementary Information

uri : https://api.aims.gov.au/data-v2.0/82fe0933-6a58-4c37-9870-77eecec0a291/files/Supplementary information-Botte et.al.pdf

Identifiers
  • global : 82fe0933-6a58-4c37-9870-77eecec0a291
ACN 633 798 857