Brief description
We generated a total of 2,313,977 16S archaeal raw reads across the 36 replicates (64,277 ± 23,335 SD per replicate). A total of 2,299,955 archaeal sequences (63,888 ± 23,473 SD per replicate) and 1,937 archaeal OTUs (54 ± 20 SD per replicate) remained for further analysis after quality filtering. The OTU data provide information on archaeal flux at an active restoration site at Mt Bold, a water catchment reserve of the Mt Lofty Ranges in South Australia, through a stagger of years and can be used accordingly.Lineage
eDNA metabarcoding : Three 25 m x 25 m quadrats were randomly selected per site, giving a total of 24 quadrats across the 8 sites. Soil was sampled from the 0-10 cm and 20-30 cm soil horizons at each quadrat. A representative 50 g sample of soil was collected at each of these 24 quadrats by pooling nine soil samples from each soil depth, including soil from open areas and under plants. These nine soil samples were pooled into a sterile plastic bag, and homogenized using a sterilized trowel. All soil samples (n = 48) were frozen on site in sterile 50 mL falcon tubes until DNA extraction, hereafter referred to as technical replicates.
Notes
CreditWe at TERN acknowledge the Traditional Owners and Custodians throughout Australia, New Zealand and all nations. We honour their profound connections to land, water, biodiversity and culture and pay our respects to their Elders past, present and emerging.
We thank A. Bissett, S. Kennedy, Z. Baruch, S. Caddy-Retalic, L. Clarke, S. Kennedy, I. Fox, M. Laws, K. McCallum, and J. McDonald for technical and field assistance. We are grateful for support from the BASE project, Australian Genome Research Facility, BioPlatforms Australia, and SA Water. This work was supported by Australian Research Council funding to AJL and MFB (DE150100542; DP150103414), and China Scholarship Council funding to DY (201408410176). We would also like to acknowledge the contribution of the BASE project partners DOI 10.4227/71/ 561c9bc670099, an initiative supported by Bioplatforms Australia with funds provided by the Australian Commonwealth Government through the National Collaborative Research Infrastructure Strategy.
This project was conducted as a part of the author's PhD. The OTU data was generated for the manuscript titled "Archaeal community responses to a decade of ecological restoration" that uses eDNA assessment to provide a significant extension to current restoration monitoring practice.
Created: 2014-12-18
Issued: 2017-09-18
Modified: 2014-07-14
Data time period: 2014-12-17 to 2014-12-18
text: IBRA Region: Flinders Lofty Block<br> Our study system was an active restoration site at Mt Bold, a water catchment reserve of the Mt Lofty Ranges in South Australia (35.07;S, 138.42 E). This catchment was dominated by an open eucalypt woodland that has historically been subjected to tree clearance and grazing that began early in the 20th century. In this context, the native understory and most of the overstory was cleared and replaced by a grassland dominated by introduced grasses. At our study site, grazing ceased in 2003 when South Australia's water utility (SA Water) took over management. SA Water has actively restored the study site since 2005, with the restoration goal of recreating the local Eucalyptus leucoxylon dominated grassy woodland community.
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Point-of-truth metadata URL- URI : geonetwork.tern.org.au/geonetwork/srv/eng/catalog.search#/metadata/3ae652ec-d46b-4d78-8f35-d3bd44ba8ecb
- global : 3ae652ec-d46b-4d78-8f35-d3bd44ba8ecb
