Data

Antarctic Krill Gonad mRNA Transcriptome

Australian Ocean Data Network
Deagle, B. ; DEAGLE, BRUCE ; POLANOWSKI, ANDREA
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=Dataset DOI&rft.title=Antarctic Krill Gonad mRNA Transcriptome&rft.identifier=Dataset DOI&rft.publisher=Australian Antarctic Data Centre&rft.description=RNA was extracted from pooled gonad tissues and tails of five sexually mature males and females, respectively, originating from the krill aquarium at the AAD in Tasmania, Australia. For RNA extractions, RNeasy mini kits (QIAGEN) were used and total RNA (8 micrograms each) was sent to Geneworks, South Australia (www.geneworks.com.au), for Illumina TruSeq 75 bp paired-end sequencing in two technical replica. Reads Yield Total Yield Krill_Male_sex_a_read1_sequence.txt 8,120,993 609,074,475 bases 1,218,148,950 bases Krill_Male_sex_a_read2_sequence.txt 8,120,993 609,074,475 bases Krill_Male_sex_b_read1_sequence.txt 10,465,586 784,918,950 bases 1,569,837,900 bases Krill_Male_sex_b_read2_sequence.txt 10,465,586 784,918,950 bases Krill_Male_tissue_a_read1_sequence.txt 7,867,804 590,085,300 bases 1,180,170,600 bases Krill_Male_tissue_a_read2_sequence.txt 7,867,804 590,085,300 bases Krill_Male_tissue_b_read1_sequence.txt 10,956,251 821,718,825 bases 1,793,118,450 bases Krill_Male_tissue_b_read2_sequence.txt 10,956,251 821,718,825 bases Krill_Female_sex_read1a_sequence.txt 29,447,654 2,208,574,050 bases 4,417,148,100 bases Krill_Female_sex_read2a_sequence.txt 29,447,654 2,208,574,050 bases Krill_Female_sex_read1b_sequence.txt 18,223,515 1,366,763,625 bases 2,733,527,250 bases Krill_Female_sex_read2b_sequence.txt 18,223,515 1,366,763,625 bases The insert size for these libraries is approx 160bp.Progress Code: completed&rft.creator=Deagle, B. &rft.creator=DEAGLE, BRUCE &rft.creator=POLANOWSKI, ANDREA &rft.date=2019&rft.coverage=westlimit=147.16809; southlimit=-43.09623; eastlimit=147.46326; northlimit=-42.86485&rft.coverage=westlimit=147.16809; southlimit=-43.09623; eastlimit=147.46326; northlimit=-42.86485&rft_rights=This metadata record is publicly available.&rft_rights=These data are publicly available for download from the provided URL&rft_rights= https://creativecommons.org/licenses/by/4.0/legalcode&rft_rights=This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4015_AAS_4015_Krill_Gonad_Transriptome when using these data. http://creativecommons.org/licenses/by/4.0/).&rft_rights=Portable Network Graphic&rft_rights=https://i.creativecommons.org/l/by/3.0/88x31.png&rft_rights=Creative Commons by Attribution logo&rft_rights=Attribution 4.0 International (CC BY 4.0)&rft_rights=Legal code for Creative Commons by Attribution 4.0 International license&rft_rights=Attribution 4.0 International (CC BY 4.0)&rft_rights= https://creativecommons.org/licenses/by/4.0/legalcode&rft.type=dataset&rft.language=English Access the data
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This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/).

Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4015_AAS_4015_Krill_Gonad_Transriptome when using these data.
http://creativecommons.org/licenses/by/4.0/).

Attribution 4.0 International (CC BY 4.0)

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This metadata record is publicly available.

These data are publicly available for download from the provided URL

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Attribution 4.0 International (CC BY 4.0)

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Brief description

RNA was extracted from pooled gonad tissues and tails of five sexually mature males and females, respectively, originating from the krill aquarium at the AAD in Tasmania, Australia. For RNA extractions, RNeasy mini kits (QIAGEN) were used and total RNA (8 micrograms each) was sent to Geneworks, South Australia (www.geneworks.com.au), for Illumina TruSeq 75 bp paired-end sequencing in two technical replica.

Reads Yield Total Yield
Krill_Male_sex_a_read1_sequence.txt 8,120,993 609,074,475 bases 1,218,148,950 bases
Krill_Male_sex_a_read2_sequence.txt 8,120,993 609,074,475 bases
Krill_Male_sex_b_read1_sequence.txt 10,465,586 784,918,950 bases 1,569,837,900 bases
Krill_Male_sex_b_read2_sequence.txt 10,465,586 784,918,950 bases

Krill_Male_tissue_a_read1_sequence.txt 7,867,804 590,085,300 bases 1,180,170,600 bases
Krill_Male_tissue_a_read2_sequence.txt 7,867,804 590,085,300 bases
Krill_Male_tissue_b_read1_sequence.txt 10,956,251 821,718,825 bases 1,793,118,450 bases
Krill_Male_tissue_b_read2_sequence.txt 10,956,251 821,718,825 bases

Krill_Female_sex_read1a_sequence.txt 29,447,654 2,208,574,050 bases 4,417,148,100 bases
Krill_Female_sex_read2a_sequence.txt 29,447,654 2,208,574,050 bases
Krill_Female_sex_read1b_sequence.txt 18,223,515 1,366,763,625 bases 2,733,527,250 bases
Krill_Female_sex_read2b_sequence.txt 18,223,515 1,366,763,625 bases

The insert size for these libraries is approx 160bp.

Lineage

Progress Code: completed

Notes

Purpose
The Antarctic krill Euphausia superba is one of the world's most abundant animal species and is a crucial component of the Southern Ocean food web where it provides the major energy source for most larger predators such as seals, whales, penguins, squid and fish. The mechanisms for determining the sex of Antarctic krill are not known. A range of potential mechanisms exist, ranging from strict genetic control by chromosomal inheritance to several possible environmental controls or combinations of genetic and environmental controls. The sex ratio of krill populations is known to vary among areas and age classes. Sex ratios in krill reaching reproductive maturity are a factor influencing potential population growth, so determining the mechanisms responsible for initial development into either sex is important for understanding the population dynamics of this important animal.

Data time period: 2012-07-01 to 2018-12-31

147.46326,-42.86485 147.46326,-43.09623 147.16809,-43.09623 147.16809,-42.86485 147.46326,-42.86485

147.315675,-42.98054

text: westlimit=147.16809; southlimit=-43.09623; eastlimit=147.46326; northlimit=-42.86485

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uri : https://data.aad.gov.au/eds/4890/download

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