Data

Amplified fragment length polymorphism analysis of asexual and sexual reproduction in Stichopus chloronotus in the Indian and Pacific Oceans

Australian Institute of Marine Science
Australian Institute of Marine Science (AIMS)
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=http://geo.aims.gov.au/geonetwork/geonetwork/srv/eng/search?uuid=b28d4efe-8837-4914-be3f-b78da3716257&rft.title=Amplified fragment length polymorphism analysis of asexual and sexual reproduction in Stichopus chloronotus in the Indian and Pacific Oceans&rft.identifier=http://geo.aims.gov.au/geonetwork/geonetwork/srv/eng/search?uuid=b28d4efe-8837-4914-be3f-b78da3716257&rft.publisher=Australian Institute of Marine Science (AIMS)&rft.description=Amplified fragment length polymorphism (AFLP) analysis indicates the importance of both asexual and sexual reproduction in the fissiparous holothurian Stichopus chloronotus (Aspidochirotida) in the Indian and Pacific Ocean.Asexual reproduction in the fissiparous holothurian species Stichopus chloronotus from eight populations (total N=149) from Madagascar and the Great Barrier Reef was investigated using Amplified fragment length polymorphism (AFLP) markers.21 polymorphic markers (obtained by two primer combinations) were used resulted in 51 genotypes in the whole sample, with up to 20 individuals (nearly all within populations) having the same genotype.West-Pacific Ocean locations were at Great Palm Island and Rib Reef on the Great Barrier Reef and Cumberland and Derder Reefs in the Torres Strait. West Indian Ocean sites were at Trou d'eau, St. Leu and Etang Sale on La Reunion, and Nosy Komba in Madagascar. To test the hypotheses that:genetic diversity in this species is reduced in the West Indian Ocean; andsome populations rely nearly exclusively on asexual reproduction.To more precisely estimate of the importance of asexual reproduction for local populations of S. chloronotus using DNA markers.Maintenance and Update Frequency: notPlanned&rft.creator=Australian Institute of Marine Science (AIMS) &rft.date=2024&rft.coverage=westlimit=143.9; southlimit=-23.6; eastlimit=152.1; northlimit=-11.5&rft.coverage=westlimit=143.9; southlimit=-23.6; eastlimit=152.1; northlimit=-11.5&rft.coverage=westlimit=113.93333; southlimit=-21.91667; eastlimit=113.93333; northlimit=-21.91667&rft.coverage=westlimit=113.93333; southlimit=-21.91667; eastlimit=113.93333; northlimit=-21.91667&rft.coverage=westlimit=152.6; southlimit=-4.33333; eastlimit=152.6; northlimit=-4.33333&rft.coverage=westlimit=152.6; southlimit=-4.33333; eastlimit=152.6; northlimit=-4.33333&rft.coverage=westlimit=178.51983; southlimit=-18.942; eastlimit=178.51983; northlimit=-18.942&rft.coverage=westlimit=178.51983; southlimit=-18.942; eastlimit=178.51983; northlimit=-18.942&rft.coverage=westlimit=48.2; southlimit=-21.3; eastlimit=55.4; northlimit=-13.3&rft.coverage=westlimit=48.2; southlimit=-21.3; eastlimit=55.4; northlimit=-13.3&rft_rights= http://creativecommons.org/licenses/by-nc/3.0/au/&rft_rights=http://i.creativecommons.org/l/by-nc/3.0/au/88x31.png&rft_rights=WWW:LINK-1.0-http--related&rft_rights=License Graphic&rft_rights=Creative Commons Attribution-NonCommercial 3.0 Australia License&rft_rights=http://creativecommons.org/international/au/&rft_rights=WWW:LINK-1.0-http--related&rft_rights=WWW:LINK-1.0-http--related&rft_rights=License Text&rft_rights=Use Limitation: All AIMS data, products and services are provided as is and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.&rft_rights=Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: Australian Institute of Marine Science (AIMS). (2009). Amplified fragment length polymorphism analysis of asexual and sexual reproduction in Stichopus chloronotus in the Indian and Pacific Oceans. https://apps.aims.gov.au/metadata/view/b28d4efe-8837-4914-be3f-b78da3716257, accessed[date-of-access].&rft_rights=Resource Usage:Use of the AIMS data is for not-for-profit applications only. All other users shall seek permission for use by contacting AIMS. Acknowledgements as prescribed must be clearly set out in the user's formal communications or publications.&rft_rights=Creative Commons Attribution-NonCommercial 3.0 Australia License http://creativecommons.org/licenses/by-nc/3.0/au&rft_subject=oceans&rft.type=dataset&rft.language=English Access the data
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Use Limitation: All AIMS data, products and services are provided "as is" and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.

Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: "Australian Institute of Marine Science (AIMS). (2009). Amplified fragment length polymorphism analysis of asexual and sexual reproduction in Stichopus chloronotus in the Indian and Pacific Oceans. https://apps.aims.gov.au/metadata/view/b28d4efe-8837-4914-be3f-b78da3716257, accessed[date-of-access]".

Resource Usage:Use of the AIMS data is for not-for-profit applications only. All other users shall seek permission for use by contacting AIMS. Acknowledgements as prescribed must be clearly set out in the user's formal communications or publications.

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Brief description

Amplified fragment length polymorphism (AFLP) analysis indicates the importance of both asexual and sexual reproduction in the fissiparous holothurian Stichopus chloronotus (Aspidochirotida) in the Indian and Pacific Ocean.Asexual reproduction in the fissiparous holothurian species Stichopus chloronotus from eight populations (total N=149) from Madagascar and the Great Barrier Reef was investigated using Amplified fragment length polymorphism (AFLP) markers.21 polymorphic markers (obtained by two primer combinations) were used resulted in 51 genotypes in the whole sample, with up to 20 individuals (nearly all within populations) having the same genotype.West-Pacific Ocean locations were at Great Palm Island and Rib Reef on the Great Barrier Reef and Cumberland and Derder Reefs in the Torres Strait. West Indian Ocean sites were at Trou d'eau, St. Leu and Etang Sale on La Reunion, and Nosy Komba in Madagascar.
To test the hypotheses that:genetic diversity in this species is reduced in the West Indian Ocean; andsome populations rely nearly exclusively on asexual reproduction.To more precisely estimate of the importance of asexual reproduction for local populations of S. chloronotus using DNA markers.

Lineage

Maintenance and Update Frequency: notPlanned

Notes

Credit
Uthicke, Sven, Dr (Principal Investigator)

Modified: 12 03 2024

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152.1,-11.5 152.1,-23.6 143.9,-23.6 143.9,-11.5 152.1,-11.5

148,-17.55

113.93333,-21.91667

113.93333,-21.91667

152.6,-4.33333

152.6,-4.33333

178.51983,-18.942

178.51983,-18.942

55.4,-13.3 55.4,-21.3 48.2,-21.3 48.2,-13.3 55.4,-13.3

51.8,-17.3

text: westlimit=143.9; southlimit=-23.6; eastlimit=152.1; northlimit=-11.5

text: westlimit=113.93333; southlimit=-21.91667; eastlimit=113.93333; northlimit=-21.91667

text: westlimit=152.6; southlimit=-4.33333; eastlimit=152.6; northlimit=-4.33333

text: westlimit=178.51983; southlimit=-18.942; eastlimit=178.51983; northlimit=-18.942

text: westlimit=48.2; southlimit=-21.3; eastlimit=55.4; northlimit=-13.3

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Other Information
Influence of asexual reproduction on the structure and dynamics of Holothuria (halodeima) atra and Strichopus chloronotus populations of the Great Barrier Reef: Uthicke S (2001) Influence of asexual reproduction on the structure and dynamics of Holothuria (halodeima) atra and Strichopus chloronotus populations of the Great Barrier Reef. Marine and Freshwater Research 52: 205-215.

local : articleId=5822

The process of asexual reproduction by transverse fission in Stichopus chloronotus (greenfish): Uthicke S (2001) The process of asexual reproduction by transverse fission in Stichopus chloronotus (greenfish). SPC Beche-de-mer Information Bulletin 14: 1.

local : articleId=8166

Identifiers
  • global : b28d4efe-8837-4914-be3f-b78da3716257
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