Amplicon sequencing of Cyp51 alleles from Pyrenophora teres using the Oxford Nanopore Technologies MinION

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Data description: Samples are barley (Hordeum vulgare) leaves infected with Pyrenophora teres collected from paddocks in Western Australia. The azole fungicide target gene Cyp51 was amplified from the samples using unique molecular identifiers according to Zulak et al., (2024) Scientific Reports 14:1 6285. Amplicons were subsequently sequenced with the Oxford Nanopore Technologies MinION sequencer using the Native Barcoding Kit V14 and the MIN114 flow cell. The resulting data file is a .fastq file containing sequence information. Collected under CCDM Project A3.4. Data collection time details: Number of files/types: 1 .fastq.gz Special software required to open files: Geographic information (if relevant): Western Australia Keywords: sequences, genes, fungal