Data

A new plant terminator element and its role in small RNA-dependent gene regulation

Queensland University of Technology
Fenselau de Felippes, Felipe ; Shand, Kylie ; Waterhouse, Peter
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.25912/RDF_1653963303638&rft.title=A new plant terminator element and its role in small RNA-dependent gene regulation&rft.identifier=10.25912/RDF_1653963303638&rft.publisher=Queensland University of Technology&rft.description=Dataset for the journal article: de Felippes FF, Shand K and Waterhouse PM (2022) Identification of a Transferrable Terminator Element That Inhibits Small RNA Production and Improves Transgene Expression Levels. Front. Plant Sci. 13:877793. . The paper describes the identification and characterisation of a new plant element that is involved with the superior performance of the HSP18.2 terminator when driving the expression of transgenes. This element acts by decreasing transcriptional read-through and small RNA generation. Data has been divided into primary folders labelled to reflect the figures where it is represented. In the case where multiple figure panels are present or multiple datasets were used in conjunction, these folders are further sub-divided and labelled accordingly. Data folders include original images, raw sequencing data, RT-qPCR raw and worked values, GFP quantification data, sRNA blots and RT-PCR gels.  &rft.creator=Fenselau de Felippes, Felipe &rft.creator=Shand, Kylie &rft.creator=Waterhouse, Peter &rft.date=2022&rft.edition=1&rft.relation=10.3389/fpls.2022.877793&rft.coverage=QUT - Gardens Point Campus, Q Block, Brisbane City, QLD 4000, Australia&rft_rights=© Queensland University of Technology, 2022.&rft_rights=Creative Commons Attribution 3.0 http://creativecommons.org/licenses/by/4.0/&rft_subject=Genome structure and regulation&rft_subject=Genetics&rft_subject=BIOLOGICAL SCIENCES&rft_subject=Gene expression (incl. microarray and other genome-wide approaches)&rft_subject=Transgenesis&rft_subject=Agricultural biotechnology&rft_subject=AGRICULTURAL, VETERINARY AND FOOD SCIENCES&rft_subject=Agricultural molecular engineering of nucleic acids and proteins&rft_subject=Plant cell and molecular biology&rft_subject=Plant biology&rft_subject=Other biological sciences&rft.type=dataset&rft.language=English Access the data
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Open Licence view details
CC-BY

Creative Commons Attribution 3.0
http://creativecommons.org/licenses/by/4.0/

© Queensland University of Technology, 2022.

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Contact Information

Postal Address:
Dr Felipe Fenselau de Felippes
Ph: +61 7 3138 7670

[email protected]

Full description

Dataset for the journal article:

de Felippes FF, Shand K and Waterhouse PM (2022) Identification of a Transferrable Terminator Element That Inhibits Small RNA Production and Improves Transgene Expression Levels. Front. Plant Sci. 13:877793. .

The paper describes the identification and characterisation of a new plant element that is involved with the superior performance of the HSP18.2 terminator when driving the expression of transgenes. This element acts by decreasing transcriptional read-through and small RNA generation.

Data has been divided into primary folders labelled to reflect the figures where it is represented. In the case where multiple figure panels are present or multiple datasets were used in conjunction, these folders are further sub-divided and labelled accordingly. Data folders include original images, raw sequencing data, RT-qPCR raw and worked values, GFP quantification data, sRNA blots and RT-PCR gels. 

Data time period: 17 12 2018 to 14 04 2022

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Spatial Coverage And Location

text: QUT - Gardens Point Campus, Q Block, Brisbane City, QLD 4000, Australia

Subjects
Agricultural, Veterinary and Food Sciences | Agricultural Biotechnology | Agricultural Molecular Engineering of Nucleic Acids and Proteins | Biological Sciences | Gene Expression (Incl. Microarray and Other Genome-Wide Approaches) | Genetics | Genome Structure and Regulation | Other Biological Sciences | Plant Biology | Plant Cell and Molecular Biology | Transgenesis |

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