Data

A new measure of particulate polysaccharides on the Great Barrier Reef

Australian Institute of Marine Science
Australian Institute of Marine Science (AIMS)
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=http://geo.aims.gov.au/geonetwork/geonetwork/srv/eng/search?uuid=274e6d26-4381-4eda-a405-64e8819b0145&rft.title=A new measure of particulate polysaccharides on the Great Barrier Reef&rft.identifier=http://geo.aims.gov.au/geonetwork/geonetwork/srv/eng/search?uuid=274e6d26-4381-4eda-a405-64e8819b0145&rft.publisher=Australian Institute of Marine Science (AIMS)&rft.description=A test using fluorescently labeled concanavalin A (FITC-conA), a lectin (a group of proteins that bind polysaccharides), was used to measure particulate polysaccharides (PPs) at 11 stations on the Great Barrier Reef. In conjunction, 96-well filtration plate technology was used to increase throughput of samples.The experiment tested a number of factors: 5 different concentrations of FITC-conA (5, 10, 20, 30, 40 µg mL-1); differences in average fluorescence (over 4 replicates) of samples using filtered and unfiltered FITC-conA; the effect of increasing incubation period of FITC-conA on fluorescence intensity (for both seasons). Concentrations of transparent exopolymer particles (TEPs) from each site in the wet season were measured using the Alcian blue technique for comparison and verification of levels. PP concentrations were compared to concentrations of chlorophyll a, pheophytin and particulate nitrogen to assess phytoplankton as a possible source of PPs.Samples were collected from each station in the wet (February 2006) and dry seasons (August 2006). Other station parameters recorded were: Secchi depth; total suspended solids (TSS); particulate organic carbon (POC); particulate phosphorous (PP); particulate nitrogen (PN); particulate polysaccharides (PPs); phaeophytin; chlorophyll a. To develop a lectin-based method for quantifying a broader scale of particulate polysaccharides to complement the existing Alcian blue method used on transparent exopolymer particles (TEPs). Sampling stations: Deloraine, Dent, Edward, Hook, Lindeman, Long, Repulse and Whitsunday Islands; Bait and Hook Reefs; Bluewater.Maintenance and Update Frequency: notPlanned&rft.creator=Australian Institute of Marine Science (AIMS) &rft.date=2024&rft.coverage=westlimit=148.85; southlimit=-20.62; eastlimit=149.22; northlimit=-19.8&rft.coverage=westlimit=148.85; southlimit=-20.62; eastlimit=149.22; northlimit=-19.8&rft_rights= http://creativecommons.org/licenses/by-nc/3.0/au/&rft_rights=http://i.creativecommons.org/l/by-nc/3.0/au/88x31.png&rft_rights=WWW:LINK-1.0-http--related&rft_rights=License Graphic&rft_rights=Creative Commons Attribution-NonCommercial 3.0 Australia License&rft_rights=http://creativecommons.org/international/au/&rft_rights=WWW:LINK-1.0-http--related&rft_rights=WWW:LINK-1.0-http--related&rft_rights=License Text&rft_rights=Use Limitation: All AIMS data, products and services are provided as is and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.&rft_rights=Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: Australian Institute of Marine Science (AIMS). (2009). A new measure of particulate polysaccharides on the Great Barrier Reef. https://apps.aims.gov.au/metadata/view/274e6d26-4381-4eda-a405-64e8819b0145, accessed[date-of-access].&rft_rights=Resource Usage:Use of the AIMS data is for not-for-profit applications only. All other users shall seek permission for use by contacting AIMS. Acknowledgements as prescribed must be clearly set out in the user's formal communications or publications.Access Constraint: intellectualPropertyRightsUse Constraint: intellectualPropertyRightsSecurity classification code: unclassifiedMetadata Usage:Access Constraint: intellectualPropertyRightsUse Constraint: intellectualPropertyRightsSecurity classification code: unclassified&rft_rights=Creative Commons Attribution-NonCommercial 3.0 Australia License http://creativecommons.org/licenses/by-nc/3.0/au&rft_subject=oceans&rft.type=dataset&rft.language=English Access the data
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Use Limitation: All AIMS data, products and services are provided "as is" and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.

Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: "Australian Institute of Marine Science (AIMS). (2009). A new measure of particulate polysaccharides on the Great Barrier Reef. https://apps.aims.gov.au/metadata/view/274e6d26-4381-4eda-a405-64e8819b0145, accessed[date-of-access]".

Resource Usage:Use of the AIMS data is for not-for-profit applications only. All other users shall seek permission for use by contacting AIMS. Acknowledgements as prescribed must be clearly set out in the user's formal communications or publications.Access Constraint: intellectualPropertyRightsUse Constraint: intellectualPropertyRightsSecurity classification code: unclassifiedMetadata Usage:Access Constraint: intellectualPropertyRightsUse Constraint: intellectualPropertyRightsSecurity classification code: unclassified

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adc@aims.gov.au

Brief description

A test using fluorescently labeled concanavalin A (FITC-conA), a lectin (a group of proteins that bind polysaccharides), was used to measure particulate polysaccharides (PPs) at 11 stations on the Great Barrier Reef. In conjunction, 96-well filtration plate technology was used to increase throughput of samples.The experiment tested a number of factors: 5 different concentrations of FITC-conA (5, 10, 20, 30, 40 µg mL-1); differences in average fluorescence (over 4 replicates) of samples using filtered and unfiltered FITC-conA; the effect of increasing incubation period of FITC-conA on fluorescence intensity (for both seasons). Concentrations of transparent exopolymer particles (TEPs) from each site in the wet season were measured using the Alcian blue technique for comparison and verification of levels. PP concentrations were compared to concentrations of chlorophyll a, pheophytin and particulate nitrogen to assess phytoplankton as a possible source of PPs.Samples were collected from each station in the wet (February 2006) and dry seasons (August 2006). Other station parameters recorded were: Secchi depth; total suspended solids (TSS); particulate organic carbon (POC); particulate phosphorous (PP); particulate nitrogen (PN); particulate polysaccharides (PPs); phaeophytin; chlorophyll a.
To develop a lectin-based method for quantifying a broader scale of particulate polysaccharides to complement the existing Alcian blue method used on transparent exopolymer particles (TEPs).
Sampling stations: Deloraine, Dent, Edward, Hook, Lindeman, Long, Repulse and Whitsunday Islands; Bait and Hook Reefs; Bluewater.

Lineage

Maintenance and Update Frequency: notPlanned

Notes

Credit
Uthicke, Sven, Dr (Principal Investigator)

Modified: 13 03 2024

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149.22,-19.8 149.22,-20.62 148.85,-20.62 148.85,-19.8 149.22,-19.8

149.035,-20.21

text: westlimit=148.85; southlimit=-20.62; eastlimit=149.22; northlimit=-19.8

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Other Information
A fluorescent lectin assay to quantify Transparent Exopolymer Particles and Marine Snow on 96-well filtration plates: Uthicke S, Llewellyn LE and Eder F (2009) A fluorescent lectin assay to quantify Transparent Exopolymer Particles and Marine Snow on 96-well filtration plates. Limnology and Oceanography: Methods 7: 449-458.

local : articleId=7890

Identifiers
  • global : 274e6d26-4381-4eda-a405-64e8819b0145
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