Data

A disease outbreak on the coral Montipora aequituberculata from the Great Barrier Reef

Australian Institute of Marine Science
Australian Institute of Marine Science (AIMS)
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=http://geo.aims.gov.au/geonetwork/geonetwork/srv/eng/search?uuid=7a07932c-2c80-4eaa-960c-fe6b4d5f71d1&rft.title=A disease outbreak on the coral Montipora aequituberculata from the Great Barrier Reef&rft.identifier=http://geo.aims.gov.au/geonetwork/geonetwork/srv/eng/search?uuid=7a07932c-2c80-4eaa-960c-fe6b4d5f71d1&rft.publisher=Australian Institute of Marine Science (AIMS)&rft.description=Three replicate Montipora colonies displaying areas of apparently healthy coral tissue and black areas with obvious diseased lesions were sampled from a small reef flat area in Nelly Bay, Magnetic Island, Australia. 'Healthy' samples were collected from normally pigmented areas of a coral colony separated by >5 cm from an observed lesion. 'Diseased' samples were collected from the boundary margin of healthy and diseased skeleton and tissue of black lesions. A permanent photographic monitoring site was established in the area in early January 2002.Samples were prepared in media designed for the isolation and enumeration of heterotrophic marine bacteria and Vibrio organisms respectively. Bacteria obtained from this study were designated: MA-HC1 and MA-HC2 for isolates obtained from healthy coral (HC) samples and MA-DC1 to MA-DC4 for isolates cultured from diseased coral (DC) samples, both grown on marine agar (MA) medium; and TCBS-DC1 to TCBS DC-4 for isolates cultured from diseased coral (DC) samples grown on TCBS growth medium.DNA was extracted from cultured isolates using the propriety reagent GeneReleaser and from coral samples using a modified UREA extraction buffer protocol. Universal bacterial primers 27F and 1492R, were used for amplification of 16S rRNA genes. PCR products were checked on a 1% agarose gel with bands of appropriate size either sequenced (bacterial isolates) or cloned (DNA extracted from corals). Duplicate PCR reactions were performed on DNA extracted from each coral sample and the products from the replicated reactions pooled.Amplified rDNA restriction analysis (ARDRA) was performed to analyse the diversity of clones within each of the healthy coral and disease coral libraries constructed.The diversity of clone libraries was further investigated by rarefaction analysis calculations. Other indices and models were used to analyse the variation of microbial diversity within clone libraries, with three different categories of measurement calculated with the EstimateS software package: Richness indices, Evenness indices, and Abundance models.Sequences of clones representing the OTU groupings generated were designated: HC-OTU1 to HC-OTU12 for clones from the healthy coral (HC) sample clone library, DC-OTU1 to DC-OTU12 for clones from the diseased coral (DC) sample clone library. Sequences were checked for chimera formation with the CHECK_CHIMERA software of the Ribosomal Database Project. Sequence data generated was analysed with the ARB software package. Tree topology was evaluated by reconstructing phylogenies using evolutionary distance (Jukes and Cantor model), maximum parsimony (ARB and DNAPARS) and maximum likelihood (ARB and fastDNAml) analyses of the aligned near full-length sequences extracted from the GenBank database. To report the results of culture-based andmolecular microbial analysis of both healthy and diseased coral tissue from Montipora corals at Magnetic Island undergoing atramentous necrosis and compare those data with 16S rDNA sequences from diseased lesions reported previously. The nucleotide sequence data appears in the GenBank database under the accession numbers: AY529868, AY529869, AY529870, AY529871, AY529872, AY529873, AY529874, AY529875, AY529876, AY529877, AY529878, AY529879, AY529880, AY529881, AY529882, AY529883, AY529884, AY529885, AY529886, AY529887, AY529888, AY529889, AY529890, AY529891, AY529892, AY529893, AY529894, AY529895, AY529896, AY529897, AY529898, AY529899, AY529900.Maintenance and Update Frequency: notPlanned&rft.creator=Australian Institute of Marine Science (AIMS) &rft.date=2024&rft.coverage=westlimit=146.8491; southlimit=-19.17018; eastlimit=146.8491; northlimit=-19.17018&rft.coverage=westlimit=146.8491; southlimit=-19.17018; eastlimit=146.8491; northlimit=-19.17018&rft_rights= http://creativecommons.org/licenses/by-nc/3.0/au/&rft_rights=http://i.creativecommons.org/l/by-nc/3.0/au/88x31.png&rft_rights=WWW:LINK-1.0-http--related&rft_rights=License Graphic&rft_rights=Creative Commons Attribution-NonCommercial 3.0 Australia License&rft_rights=http://creativecommons.org/international/au/&rft_rights=WWW:LINK-1.0-http--related&rft_rights=WWW:LINK-1.0-http--related&rft_rights=License Text&rft_rights=Use Limitation: All AIMS data, products and services are provided as is and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.&rft_rights=Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: Australian Institute of Marine Science (AIMS). (2009). A disease outbreak on the coral Montipora aequituberculata from the Great Barrier Reef. https://apps.aims.gov.au/metadata/view/7a07932c-2c80-4eaa-960c-fe6b4d5f71d1, accessed[date-of-access].&rft_rights=Resource Usage:Use of the AIMS data is for not-for-profit applications only. All other users shall seek permission for use by contacting AIMS. Acknowledgements as prescribed must be clearly set out in the user's formal communications or publications.Access Constraint: intellectualPropertyRightsUse Constraint: intellectualPropertyRightsSecurity classification code: unclassifiedMetadata Usage:Access Constraint: intellectualPropertyRightsUse Constraint: intellectualPropertyRightsSecurity classification code: unclassified&rft_rights=Creative Commons Attribution-NonCommercial 3.0 Australia License http://creativecommons.org/licenses/by-nc/3.0/au&rft_subject=oceans&rft.type=dataset&rft.language=English Access the data

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Use Limitation: All AIMS data, products and services are provided "as is" and AIMS does not warrant their fitness for a particular purpose or non-infringement. While AIMS has made every reasonable effort to ensure high quality of the data, products and services, to the extent permitted by law the data, products and services are provided without any warranties of any kind, either expressed or implied, including without limitation any implied warranties of title, merchantability, and fitness for a particular purpose or non-infringement. AIMS make no representation or warranty that the data, products and services are accurate, complete, reliable or current. To the extent permitted by law, AIMS exclude all liability to any person arising directly or indirectly from the use of the data, products and services.

Attribution: Format for citation of metadata sourced from Australian Institute of Marine Science (AIMS) in a list of reference is as follows: "Australian Institute of Marine Science (AIMS). (2009). A disease outbreak on the coral Montipora aequituberculata from the Great Barrier Reef. https://apps.aims.gov.au/metadata/view/7a07932c-2c80-4eaa-960c-fe6b4d5f71d1, accessed[date-of-access]".

Resource Usage:Use of the AIMS data is for not-for-profit applications only. All other users shall seek permission for use by contacting AIMS. Acknowledgements as prescribed must be clearly set out in the user's formal communications or publications.Access Constraint: intellectualPropertyRightsUse Constraint: intellectualPropertyRightsSecurity classification code: unclassifiedMetadata Usage:Access Constraint: intellectualPropertyRightsUse Constraint: intellectualPropertyRightsSecurity classification code: unclassified

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reception@aims.gov.au
adc@aims.gov.au

Brief description

Three replicate Montipora colonies displaying areas of apparently healthy coral tissue and black areas with obvious diseased lesions were sampled from a small reef flat area in Nelly Bay, Magnetic Island, Australia. 'Healthy' samples were collected from normally pigmented areas of a coral colony separated by >5 cm from an observed lesion. 'Diseased' samples were collected from the boundary margin of healthy and diseased skeleton and tissue of black lesions. A permanent photographic monitoring site was established in the area in early January 2002.Samples were prepared in media designed for the isolation and enumeration of heterotrophic marine bacteria and Vibrio organisms respectively. Bacteria obtained from this study were designated: MA-HC1 and MA-HC2 for isolates obtained from healthy coral (HC) samples and MA-DC1 to MA-DC4 for isolates cultured from diseased coral (DC) samples, both grown on marine agar (MA) medium; and TCBS-DC1 to TCBS DC-4 for isolates cultured from diseased coral (DC) samples grown on TCBS growth medium.DNA was extracted from cultured isolates using the propriety reagent GeneReleaser and from coral samples using a modified UREA extraction buffer protocol. Universal bacterial primers 27F and 1492R, were used for amplification of 16S rRNA genes. PCR products were checked on a 1% agarose gel with bands of appropriate size either sequenced (bacterial isolates) or cloned (DNA extracted from corals). Duplicate PCR reactions were performed on DNA extracted from each coral sample and the products from the replicated reactions pooled.Amplified rDNA restriction analysis (ARDRA) was performed to analyse the diversity of clones within each of the healthy coral and disease coral libraries constructed.The diversity of clone libraries was further investigated by rarefaction analysis calculations. Other indices and models were used to analyse the variation of microbial diversity within clone libraries, with three different categories of measurement calculated with the EstimateS software package: Richness indices, Evenness indices, and Abundance models.Sequences of clones representing the OTU groupings generated were designated: HC-OTU1 to HC-OTU12 for clones from the healthy coral (HC) sample clone library, DC-OTU1 to DC-OTU12 for clones from the diseased coral (DC) sample clone library. Sequences were checked for chimera formation with the CHECK_CHIMERA software of the Ribosomal Database Project. Sequence data generated was analysed with the ARB software package. Tree topology was evaluated by reconstructing phylogenies using evolutionary distance (Jukes and Cantor model), maximum parsimony (ARB and DNAPARS) and maximum likelihood (ARB and fastDNAml) analyses of the aligned near full-length sequences extracted from the GenBank database.
To report the results of culture-based andmolecular microbial analysis of both healthy and diseased coral tissue from Montipora corals at Magnetic Island undergoing atramentous necrosis and compare those data with 16S rDNA sequences from diseased lesions reported previously.
The nucleotide sequence data appears in the GenBank database under the accession numbers: AY529868, AY529869, AY529870, AY529871, AY529872, AY529873, AY529874, AY529875, AY529876, AY529877, AY529878, AY529879, AY529880, AY529881, AY529882, AY529883, AY529884, AY529885, AY529886, AY529887, AY529888, AY529889, AY529890, AY529891, AY529892, AY529893, AY529894, AY529895, AY529896, AY529897, AY529898, AY529899, AY529900.

Lineage

Maintenance and Update Frequency: notPlanned

Notes

Credit
Bourne, David G, Dr (Principal Investigator)

Modified: 15 07 2024

This dataset is part of a larger collection

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146.8491,-19.17018

146.8491,-19.17018

text: westlimit=146.8491; southlimit=-19.17018; eastlimit=146.8491; northlimit=-19.17018

Subjects
oceans |

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Other Information
Microbiological assessment of a disease outbreak on corals from Magnetic Island (Great Barrier Reef, Australia).: Bourne DG (2005) Microbiological assessment of a disease outbreak on corals from Magnetic Island (Great Barrier Reef, Australia). Coral Reefs 24: 304-312.

local : articleId=6827

Identifiers
  • global : 7a07932c-2c80-4eaa-960c-fe6b4d5f71d1