Data

20230911 PFEV miRNA datasets

The University of Queensland
Dr Kelly Chee (Aggregated by) Dr Kelly Chee (Aggregated by)
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.48610/cece534&rft.title=20230911 PFEV miRNA datasets&rft.identifier=RDM ID: 1ad51f30-504e-11ee-a2b4-7d9be4381132&rft.publisher=The University of Queensland&rft.description=The Nanostring nCounter® Human v3 miRNA expression assay panel includes 798 miRNA probes to screen 827 miRNAs that are highly curated in miRBase 22 (released 12th March 2018). Unique oligonucleotide tags were ligated onto native miRNAs in a total RNA sample, followed by hybridisation (12 – 30 hours) of tagged miRNAs and capture/reporter probes with colour-coded sequence specific to each miRNA target. Post-hybridisation samples were then loaded onto a SPRINT cartridge, and processed on the Nanostring nCounter® SPRINT Profiler (NanaString Technologies, Seattle, WA, USA). Data was acquired through instrument automated fluorescence microscope scanning with direct counts of target-probe complexes, and analysed by Nanostring nSolver™ software.&rft.creator=Dr Kelly Chee&rft.creator=Dr Kelly Chee&rft.date=2023&rft_rights= https://guides.library.uq.edu.au/deposit-your-data/license-reuse-data-agreement&rft_subject=eng&rft_subject=Gene expression (incl. microarray and other genome-wide approaches)&rft_subject=Genetics&rft_subject=BIOLOGICAL SCIENCES&rft_subject=Genomics&rft_subject=Cancer genetics&rft_subject=Oncology and carcinogenesis&rft_subject=BIOMEDICAL AND CLINICAL SCIENCES&rft.type=dataset&rft.language=English Access the data
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Contact Information

t.chee@uq.edu.au
Prince Charles Hospital Northside Clinical Unit

Full description

The Nanostring nCounter® Human v3 miRNA expression assay panel includes 798 miRNA probes to screen 827 miRNAs that are highly curated in miRBase 22 (released 12th March 2018). Unique oligonucleotide tags were ligated onto native miRNAs in a total RNA sample, followed by hybridisation (12 – 30 hours) of tagged miRNAs and capture/reporter probes with colour-coded sequence specific to each miRNA target. Post-hybridisation samples were then loaded onto a SPRINT cartridge, and processed on the Nanostring nCounter® SPRINT Profiler (NanaString Technologies, Seattle, WA, USA). Data was acquired through instrument automated fluorescence microscope scanning with direct counts of target-probe complexes, and analysed by Nanostring nSolver™ software.

Issued: 09 2023

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Subjects
Biological Sciences | Biomedical and Clinical Sciences | Cancer Genetics | Gene Expression (Incl. Microarray and Other Genome-Wide Approaches) | Genetics | Genomics | Oncology and Carcinogenesis | eng |

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local : UQ:289097

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