Data

2010/11 VMS Geonomics sampling - data collected from the VMS (Voyage Marine Science) voyage of the Aurora Australis

Australian Antarctic Data Centre
WILLIAMS, TIM ; WILKINS, DAVID
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.4225/15/59a601e430067&rft.title=2010/11 VMS Geonomics sampling - data collected from the VMS (Voyage Marine Science) voyage of the Aurora Australis&rft.identifier=10.4225/15/59a601e430067&rft.publisher=Australian Antarctic Data Centre&rft.description=Purpose of future metagenomic (DNA), metaproteomic (protein) and metatranscriptomic (RNA) analysis: For each sample, two drums (~200L each) of seawater were collected. Samples were taken from CTD sites, and surface samples (2m depth) taken at each of these sites. At most of these CTD sites, a deeper sample was taken according to the location of the DCM at that site. The 200L seawater is pumped through a 20 micron mesh to remove the largest particles, then the biomass is collected on three consecutive filters corresponding to decreasing pore size (3.0 microns, 0.8 microns, 0.1 microns). This is repeated for each sample using the second 200L of seawater to generate duplicates for each sample. The overall aim is to determine the identity of microbes present in the Southern Ocean, and what microbial metabolic processes are in operation. In other words: who is there, and what they are doing. Special emphasis was placed on the SR3 transect. Samples were collected as below. For each sample, a total of six filters were obtained (3x pore sizes, 2x replicates). Each filter is stored in a storage buffer in a 50mL tube, and placed at -80 degrees C for the remainder of the voyage.&rft.creator=WILLIAMS, TIM &rft.creator=WILKINS, DAVID &rft.date=2011&rft.coverage=northlimit=-42.0; southlimit=-67.0; westlimit=140.0; eastLimit=150.0; projection=WGS84&rft.coverage=northlimit=-42.0; southlimit=-67.0; westlimit=140.0; eastLimit=150.0; projection=WGS84&rft_rights=This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=VMS_Genomics when using these data.&rft_subject=oceans&rft_subject=MARINE ENVIRONMENT MONITORING&rft_subject=EARTH SCIENCE&rft_subject=OCEANS&rft_subject=PROTISTS&rft_subject=BIOLOGICAL CLASSIFICATION&rft_subject=VMS&rft_subject=Genomics&rft_subject=CTD > Conductivity, Temperature, Depth&rft_subject=NISKIN BOTTLES&rft_subject=SHIPS&rft_subject=R/V AA > R/V Aurora Australis&rft_subject=OCEAN > SOUTHERN OCEAN > Mertz Polynya&rft_subject=CONTINENT > ANTARCTICA&rft_subject=GEOGRAPHIC REGION > POLAR&rft_place=Hobart&rft.type=dataset&rft.language=English Access the data
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This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=VMS_Genomics when using these data.

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Brief description

Purpose of future metagenomic (DNA), metaproteomic (protein) and metatranscriptomic (RNA) analysis: For each sample, two drums (~200L each) of seawater were collected. Samples were taken from CTD sites, and surface samples (2m depth) taken at each of these sites. At most of these CTD sites, a deeper sample was taken according to the location of the DCM at that site. The 200L seawater is pumped through a 20 micron mesh to remove the largest particles, then the biomass is collected on three consecutive filters corresponding to decreasing pore size (3.0 microns, 0.8 microns, 0.1 microns). This is repeated for each sample using the second 200L of seawater to generate duplicates for each sample. The overall aim is to determine the identity of microbes present in the Southern Ocean, and what microbial metabolic processes are in operation. In other words: who is there, and what they are doing. Special emphasis was placed on the SR3 transect. Samples were collected as below. For each sample, a total of six filters were obtained (3x pore sizes, 2x replicates). Each filter is stored in a storage buffer in a 50mL tube, and placed at -80 degrees C for the remainder of the voyage.

Issued: 2011-05-17

Data time period: 2011-01-04 to 2011-02-06

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150,-42 150,-67 140,-67 140,-42 150,-42

145,-54.5

text: northlimit=-42.0; southlimit=-67.0; westlimit=140.0; eastLimit=150.0; projection=WGS84

Subjects
BIOLOGICAL CLASSIFICATION | CONTINENT > ANTARCTICA | CTD > Conductivity, Temperature, Depth | EARTH SCIENCE | GEOGRAPHIC REGION > POLAR | Genomics | MARINE ENVIRONMENT MONITORING | NISKIN BOTTLES | OCEAN > SOUTHERN OCEAN > Mertz Polynya | OCEANS | PROTISTS | R/V AA > R/V Aurora Australis | SHIPS | VMS | oceans |

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AU-ANL:PEAU : http://nla.gov.au/nla.party-617536

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