Data

2010/11 VMS Geonomics sampling - data collected from the VMS (Voyage Marine Science) voyage of the Aurora Australis

Australian Antarctic Division
Williams, T. and Wilkins, D. ; WILLIAMS, TIM ; WILKINS, DAVID
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.4225/15/59a601e430067&rft.title=2010/11 VMS Geonomics sampling - data collected from the VMS (Voyage Marine Science) voyage of the Aurora Australis&rft.identifier=10.4225/15/59a601e430067&rft.publisher=Australian Antarctic Data Centre&rft.description=Purpose of future metagenomic (DNA), metaproteomic (protein) and metatranscriptomic (RNA) analysis:For each sample, two drums (~200L each) of seawater were collected. Samples were taken from CTD sites, and surface samples (2m depth) taken at each of these sites. At most of these CTD sites, a deeper sample was taken according to the location of the DCM at that site. The 200L seawater is pumped through a 20 micron mesh to remove the largest particles, then the biomass is collected on three consecutive filters corresponding to decreasing pore size (3.0 microns, 0.8 microns, 0.1 microns). This is repeated for each sample using the second 200L of seawater to generate duplicates for each sample. The overall aim is to determine the identity of microbes present in the Southern Ocean, and what microbial metabolic processes are in operation. In other words: who is there, and what they are doing. Special emphasis was placed on the SR3 transect.Samples were collected as below. For each sample, a total of six filters were obtained (3x pore sizes, 2x replicates). Each filter is stored in a storage buffer in a 50mL tube, and placed at -80 degrees C for the remainder of the voyage.Progress Code: completed&rft.creator=Williams, T. and Wilkins, D. &rft.creator=WILLIAMS, TIM &rft.creator=WILKINS, DAVID &rft.date=2011&rft.coverage=westlimit=140.0; southlimit=-67.0; eastlimit=150.0; northlimit=-42.0&rft.coverage=westlimit=140.0; southlimit=-67.0; eastlimit=150.0; northlimit=-42.0&rft.coverage=uplimit=4500; downlimit=0&rft.coverage=uplimit=4500; downlimit=0&rft_rights=These data are publicly available for download from the provided URL.&rft_rights=Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/legalcode&rft_rights=This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=VMS_Genomics when using these data.&rft_rights=This metadata record is publicly available.&rft_subject=oceans&rft_subject=EARTH SCIENCE > OCEANS > MARINE ENVIRONMENT MONITORING&rft_subject=EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PROTISTS&rft_subject=VMS&rft_subject=Genomics&rft_subject=CTD > Conductivity, Temperature, Depth&rft_subject=NISKIN BOTTLES&rft_subject=SHIPS&rft_subject=R/V AA > R/V Aurora Australis&rft_subject=AMD/AU&rft_subject=CEOS&rft_subject=AMD&rft_subject=OCEAN > SOUTHERN OCEAN > Mertz Polynya&rft_subject=CONTINENT > ANTARCTICA&rft_subject=GEOGRAPHIC REGION > POLAR&rft.type=dataset&rft.language=English Access the data
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Attribution 4.0 International (CC BY 4.0)
https://creativecommons.org/licenses/by/4.0/legalcode

These data are publicly available for download from the provided URL.

This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=VMS_Genomics when using these data.

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Purpose of future metagenomic (DNA), metaproteomic (protein) and metatranscriptomic (RNA) analysis:

For each sample, two drums (~200L each) of seawater were collected. Samples were taken from CTD sites, and surface samples (2m depth) taken at each of these sites. At most of these CTD sites, a deeper sample was taken according to the location of the DCM at that site. The 200L seawater is pumped through a 20 micron mesh to remove the largest particles, then the biomass is collected on three consecutive filters corresponding to decreasing pore size (3.0 microns, 0.8 microns, 0.1 microns). This is repeated for each sample using the second 200L of seawater to generate duplicates for each sample. The overall aim is to determine the identity of microbes present in the Southern Ocean, and what microbial metabolic processes are in operation. In other words: who is there, and what they are doing. Special emphasis was placed on the SR3 transect.
Samples were collected as below. For each sample, a total of six filters were obtained (3x pore sizes, 2x replicates). Each filter is stored in a storage buffer in a 50mL tube, and placed at -80 degrees C for the remainder of the voyage.

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Progress Code: completed

Data time period: 2011-01-04 to 2011-02-06

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150,-42 150,-67 140,-67 140,-42 150,-42

145,-54.5

text: westlimit=140.0; southlimit=-67.0; eastlimit=150.0; northlimit=-42.0

text: uplimit=4500; downlimit=0

Subjects
AMD | AMD/AU | CEOS | CONTINENT > ANTARCTICA | CTD > Conductivity, Temperature, Depth | EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PROTISTS | EARTH SCIENCE > OCEANS > MARINE ENVIRONMENT MONITORING | GEOGRAPHIC REGION > POLAR | Genomics | NISKIN BOTTLES | OCEAN > SOUTHERN OCEAN > Mertz Polynya | R/V AA > R/V Aurora Australis | SHIPS | VMS | oceans |

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Other Information
Download the data (GET DATA)

url : https://data.aad.gov.au/eds/3655/download

Public information for AAS project 3145 (PROJECT HOME PAGE)

url : https://projects.aad.gov.au/search_projects_results.cfm?project_no=AAS_3145

Voyage details with maps, schedule, events and projects undertaken (VIEW RELATED INFORMATION)

url : http://data.aad.gov.au/aadc/voyages/display_voyage.cfm?voyage_id=555

Citation reference for this metadata record and dataset (VIEW RELATED INFORMATION)

url : http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=VMS_Genomics

Identifiers
ACN 633 798 857