Data

16.4T MRI of nephrectomy sample - updated

The University of Queensland
Dr Nyoman Kurniawan (Aggregated by) Dr Nyoman Kurniawan (Aggregated by)
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.48610/7da7765&rft.title=16.4T MRI of nephrectomy sample - updated&rft.identifier=RDM ID: 10ca5e49-279f-4df6-9ca2-94df008e1e84&rft.publisher=The University of Queensland&rft.description=Tissue samples were obtained from whole kidney post-nephrectomy without flushing the blood, fixed in formalin for 24 h and washed with saline for 3 d at 4°C prior to scanning. Samples were scanned at room temperature using a Bruker Biospec 16.4 T MRI at the Centre for Advanced Imaging, The University of Queensland. This scanner is equipped with a Micro2.5 gradient (1.5 T/m at 60 A). Microimaging coils appropriate to the size of the samples were used, these included a 15×30 mm surface coil, a 15 mm and a 10 mm volume coil. Samples were either placed in saline or wrapped with paraffin film to minimise air artefacts. MRI was performed using a 3D T1/T2*-weighted gradient echo (GRE) sequence with repetition time TR = 150 ms, echo time TE = 6.2 ms, flip angle 60°. Typically, the field-of-view was ~2.4×1.2×1.2 cm with the matrix sizes set to produce images at 30 μm 3D isotropic resolutions. The acquisition times were approximately 27 h (number of excitations (NEX) = 4). A sine windowing function was applied to the K-space data prior to the Fourier transform to reduce the noise in the 30 μm images.&rft.creator=Dr Nyoman Kurniawan&rft.creator=Dr Nyoman Kurniawan&rft.date=2025&rft_rights= https://guides.library.uq.edu.au/deposit-your-data/license-reuse-data-agreement&rft_subject=eng&rft_subject=Nephrology and urology&rft_subject=Clinical sciences&rft_subject=BIOMEDICAL AND CLINICAL SCIENCES&rft_subject=Biomedical imaging&rft_subject=Biomedical engineering&rft_subject=ENGINEERING&rft_subject=Image processing&rft_subject=Computer vision and multimedia computation&rft_subject=INFORMATION AND COMPUTING SCIENCES&rft.type=dataset&rft.language=English Access the data
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[email protected]
Australian Institute for Bioengineering and Nanotechnology

Full description

Tissue samples were obtained from whole kidney post-nephrectomy without flushing the blood, fixed in formalin for 24 h and washed with saline for 3 d at 4°C prior to scanning. Samples were scanned at room temperature using a Bruker Biospec 16.4 T MRI at the Centre for Advanced Imaging, The University of Queensland. This scanner is equipped with a Micro2.5 gradient (1.5 T/m at 60 A). Microimaging coils appropriate to the size of the samples were used, these included a 15×30 mm surface coil, a 15 mm and a 10 mm volume coil. Samples were either placed in saline or wrapped with paraffin film to minimise air artefacts. MRI was performed using a 3D T1/T2*-weighted gradient echo (GRE) sequence with repetition time TR = 150 ms, echo time TE = 6.2 ms, flip angle 60°. Typically, the field-of-view was ~2.4×1.2×1.2 cm with the matrix sizes set to produce images at 30 μm 3D isotropic resolutions. The acquisition times were approximately 27 h (number of excitations (NEX) = 4). A sine windowing function was applied to the K-space data prior to the Fourier transform to reduce the noise in the 30 μm images.

Issued: 2025

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Subjects
Biomedical and Clinical Sciences | Biomedical Engineering | Biomedical Imaging | Clinical Sciences | Computer Vision and Multimedia Computation | Engineering | Information and Computing Sciences | Image Processing | Nephrology and Urology | eng |

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Other Information
Counting glomeruli in human kidney specimens using ex vivo MRI without contrast agents

local : UQ:3f10ace

Kurniawan, Nyoman D., Amar, Aurel J., Cullen‐McEwen, Luise A., Combes, Alexander N., Hayatudin, Raeesah, Kassianos, Andrew J., Du, Jiaxin, Gazzard, Sarah E., Healy, Helen G., Hoy, Wendy E., Bertram, John F. and Reutens, David C. (2025). Counting glomeruli in human kidney specimens using ex vivo MRI without contrast agents. Magnetic Resonance in Medicine, 94 (6) mrm.30642, 2519-2528. doi: 10.1002/mrm.30642

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local : UQ:289097

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