Data

Wild barley QTL validation: 2025 multi-environment trial phenotypes of BC₃F₄ NILs and 4H GPE QTL introgression haplotype data for UAB-27 (BC₁F₆) in Compass background.

Adelaide University
Tura, Habtamu Seboka ; Pham, Anh
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=info:doi10.25909/32320872.v2&rft.title=Wild barley QTL validation: 2025 multi-environment trial phenotypes of BC₃F₄ NILs and 4H GPE QTL introgression haplotype data for UAB-27 (BC₁F₆) in Compass background.&rft.identifier=10.25909/32320872.v2&rft.publisher=Adelaide University&rft.description=This dataset accompanies project UOA2401-005RTX (Validation and deployment of novel genomic regions from wild barley to enhance grain yield and yield components in domesticated barley), funded by GRDC and led by The University of Adelaide.A previous GRDC project (UOA2001-003RTX) identified two wild barley QTLs introgressed into the Australian cultivar Compass: a grains-per-ear (GPE) QTL on chromosome 4H (UAB-27 lineage) and a dry biomass QTL on chromosome 3H (UAB-70 lineage). Yield gains were inconsistent across environments, indicating residual linkage drag. The current project aims to precisely characterise these two QTLs, validate their effects in advanced backcross NILs across Australian environments, and develop characterised germplasm and molecular markers for use by Australian barley breeders.In 2025, 43 BC₃F₄ NILs (UAB-27 and UAB-70 lineages) and 8 controls were evaluated in a partially replicated row × column trial at Northam (WA), Roseworthy (SA), and Narrabri (NSW), managed by Australian Grain Technologies (AGT). The deposit provides plot-level phenotypes and per-site ASReml-R BLUPs for yield and yield-component traits.In parallel, whole-genome sequencing of the UAB-27 BC₁F₆ line and a comparative panel of UAB lines, wild donors (HEB-06, HEB-16, HEB-25), and Compass was used to confirm the 4H introgression and identify diagnostic SNPs for KASP marker development. The 4H SNP, haplotype, and candidate-gene tables and the haplotype structure figure are included.&rft.creator=Tura, Habtamu Seboka &rft.creator=Pham, Anh &rft.edition=2&rft_rights= https://www.adelaide.edu.au/library/restricted-access&rft_subject=Crop and pasture improvement (incl. selection and breeding)&rft_subject=Genotype&rft_subject=Barley/wild barley&rft_subject=Wild Allele&rft_subject=QTL&rft_subject=NIL&rft_subject=Compass&rft_subject=UAB-27&rft_subject=UAB-70&rft_subject=Grain Yield&rft_subject=TGW&rft_subject=biomass&rft_subject=GPE&rft_subject=SNP&rft_subject=Haplotype&rft.type=dataset&rft.language=English Access the data
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This dataset accompanies project UOA2401-005RTX (Validation and deployment of novel genomic regions from wild barley to enhance grain yield and yield components in domesticated barley), funded by GRDC and led by The University of Adelaide.

A previous GRDC project (UOA2001-003RTX) identified two wild barley QTLs introgressed into the Australian cultivar Compass: a grains-per-ear (GPE) QTL on chromosome 4H (UAB-27 lineage) and a dry biomass QTL on chromosome 3H (UAB-70 lineage). Yield gains were inconsistent across environments, indicating residual linkage drag. The current project aims to precisely characterise these two QTLs, validate their effects in advanced backcross NILs across Australian environments, and develop characterised germplasm and molecular markers for use by Australian barley breeders.

In 2025, 43 BC₃F₄ NILs (UAB-27 and UAB-70 lineages) and 8 controls were evaluated in a partially replicated row × column trial at Northam (WA), Roseworthy (SA), and Narrabri (NSW), managed by Australian Grain Technologies (AGT). The deposit provides plot-level phenotypes and per-site ASReml-R BLUPs for yield and yield-component traits.

In parallel, whole-genome sequencing of the UAB-27 BC₁F₆ line and a comparative panel of UAB lines, wild donors (HEB-06, HEB-16, HEB-25), and Compass was used to confirm the 4H introgression and identify diagnostic SNPs for KASP marker development. The 4H SNP, haplotype, and candidate-gene tables and the haplotype structure figure are included.

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Subjects
Barley/wild barley | Compass | Crop and pasture improvement (incl. selection and breeding) | GPE | Genotype | Grain Yield | Haplotype | NIL | QTL | SNP | TGW | UAB-27 | UAB-70 | Wild Allele | biomass |

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Identifiers
ACN 633 798 857