Data

Voyage 4 of the Aurora Australis, 2018 environmental DNA samples analysed with group specific metabarcoding markers

Australian Ocean Data Network
Suter, L., Polanowski, A. and Deagle, B. ; SUTER, LEONIE ; POLANOWSKI, ANDREA ; DEAGLE, BRUCE
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=Dataset DOI&rft.title=Voyage 4 of the Aurora Australis, 2018 environmental DNA samples analysed with group specific metabarcoding markers&rft.identifier=Dataset DOI&rft.publisher=Australian Antarctic Data Centre&rft.description=In March 2018, 23 environmental DNA (eDNA) samples (2 L of filtered seawater) were collected between Hobart, Tasmania and subantarctic Macquarie Island. These samples were processed using six different genetic metabarcoding markers targeting different taxonomic groups within the metazoan clade: A broad cytochrome c oxidase subunit I (COI) marker targeting all metazoans, and five different 16S markers targeting fish, cephalopods and crustaceans (one degenerate marker), fish (two markers of different lengths), cephalopods (one marker) and crustaceans (one marker). The aim of this study was to identify an ideal set of molecular markers to identify as many metazoan species as possible from small environmental samples, with a particular focus on vertebrates, crustaceans and cephalopods. The data and methods are described in the word file V4 2018 eDNA group specific markers.docx, results are summarised in the excel file Marker.detection.xlsx and additional sample information is in the excel files 2018_11_07_eDNA-sample-info.xls and sample.map.csv. Each genetic marker used in this study has its own folder, containing the raw FASTQ sequencing data, the processed FASTA sequencing data, the bioinformatics processing pipeline, the zOTU fasta file, BLAST output, MEGAN output and curated zOTU table. For further explanations please refer to the word file V4 2018 eDNA group specific markers.docx.Progress Code: completed&rft.creator=Suter, L., Polanowski, A. and Deagle, B. &rft.creator=SUTER, LEONIE &rft.creator=POLANOWSKI, ANDREA &rft.creator=DEAGLE, BRUCE &rft.date=2021&rft.coverage=westlimit=147.3859956; southlimit=-54.7236529; eastlimit=158.9606394; northlimit=-42.9491591&rft.coverage=westlimit=147.3859956; southlimit=-54.7236529; eastlimit=158.9606394; northlimit=-42.9491591&rft_rights=This metadata record is publicly available.&rft_rights=These data are not yet publicly available for download.&rft_rights= https://creativecommons.org/licenses/by/4.0/legalcode&rft_rights=This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4556_group_specific_eDNA_V4_2018 when using these data. http://creativecommons.org/licenses/by/4.0/).&rft_rights=Portable Network Graphic&rft_rights=https://i.creativecommons.org/l/by/3.0/88x31.png&rft_rights=Creative Commons by Attribution logo&rft_rights=Attribution 4.0 International (CC BY 4.0)&rft_rights=Legal code for Creative Commons by Attribution 4.0 International license&rft_rights=Attribution 4.0 International (CC BY 4.0)&rft_rights= https://creativecommons.org/licenses/by/4.0/legalcode&rft.type=dataset&rft.language=English Access the data
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This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/).

Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4556_group_specific_eDNA_V4_2018 when using these data.
http://creativecommons.org/licenses/by/4.0/).

Attribution 4.0 International (CC BY 4.0)

https://creativecommons.org/licenses/by/4.0/legalcode

This metadata record is publicly available.

These data are not yet publicly available for download.

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Attribution 4.0 International (CC BY 4.0)

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Brief description

In March 2018, 23 environmental DNA (eDNA) samples (2 L of filtered seawater) were collected between Hobart, Tasmania and subantarctic Macquarie Island. These samples were processed using six different genetic metabarcoding markers targeting different taxonomic groups within the metazoan clade: A broad cytochrome c oxidase subunit I (COI) marker targeting all metazoans, and five different 16S markers targeting fish, cephalopods and crustaceans (one degenerate marker), fish (two markers of different lengths), cephalopods (one marker) and crustaceans (one marker). The aim of this study was to identify an ideal set of molecular markers to identify as many metazoan species as possible from small environmental samples, with a particular focus on vertebrates, crustaceans and cephalopods.

The data and methods are described in the word file "V4 2018 eDNA group specific markers.docx", results are summarised in the excel file "Marker.detection.xlsx" and additional sample information is in the excel files "2018_11_07_eDNA-sample-info.xls" and "sample.map.csv". Each genetic marker used in this study has its own folder, containing the raw FASTQ sequencing data, the processed FASTA sequencing data, the bioinformatics processing pipeline, the zOTU fasta file, BLAST output, MEGAN output and curated zOTU table. For further explanations please refer to the word file "V4 2018 eDNA group specific markers.docx".

Lineage

Progress Code: completed

Notes

Purpose
The aim of this study was to identify an ideal set of molecular markers to identify as many metazoan species as possible from small environmental samples, with a particular focus on vertebrates, crustaceans and cephalopods.

Data time period: 2018-03-09 to 2018-03-23

158.9606394,-42.9491591 158.9606394,-54.7236529 147.3859956,-54.7236529 147.3859956,-42.9491591 158.9606394,-42.9491591

153.1733175,-48.836406

text: westlimit=147.3859956; southlimit=-54.7236529; eastlimit=158.9606394; northlimit=-42.9491591

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uri : https://data.aad.gov.au/eds/5221/download

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