Data

Voyage 4 of the Aurora Australis, 2018 environmental DNA samples analysed with group specific metabarcoding markers

Australian Ocean Data Network
Suter, L., Polanowski, A. and Deagle, B. ; SUTER, LEONIE ; POLANOWSKI, ANDREA ; DEAGLE, BRUCE
Viewed: [[ro.stat.viewed]] Cited: [[ro.stat.cited]] Accessed: [[ro.stat.accessed]]
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=http://data.aad.gov.au/metadata/records/AAS_4556_group_specific_eDNA_V4_2018&rft.title=Voyage 4 of the Aurora Australis, 2018 environmental DNA samples analysed with group specific metabarcoding markers&rft.identifier=http://data.aad.gov.au/metadata/records/AAS_4556_group_specific_eDNA_V4_2018&rft.publisher=Australian Antarctic Data Centre&rft.description=In March 2018, 23 environmental DNA (eDNA) samples (2 L of filtered seawater) were collected between Hobart, Tasmania and subantarctic Macquarie Island. These samples were processed using six different genetic metabarcoding markers targeting different taxonomic groups within the metazoan clade: A broad cytochrome c oxidase subunit I (COI) marker targeting all metazoans, and five different 16S markers targeting fish, cephalopods and crustaceans (one degenerate marker), fish (two markers of different lengths), cephalopods (one marker) and crustaceans (one marker). The aim of this study was to identify an ideal set of molecular markers to identify as many metazoan species as possible from small environmental samples, with a particular focus on vertebrates, crustaceans and cephalopods. The data and methods are described in the word file V4 2018 eDNA group specific markers.docx, results are summarised in the excel file Marker.detection.xlsx and additional sample information is in the excel files 2018_11_07_eDNA-sample-info.xls and sample.map.csv. Each genetic marker used in this study has its own folder, containing the raw FASTQ sequencing data, the processed FASTA sequencing data, the bioinformatics processing pipeline, the zOTU fasta file, BLAST output, MEGAN output and curated zOTU table. For further explanations please refer to the word file V4 2018 eDNA group specific markers.docx.Progress Code: completed&rft.creator=Suter, L., Polanowski, A. and Deagle, B. &rft.creator=SUTER, LEONIE &rft.creator=POLANOWSKI, ANDREA &rft.creator=DEAGLE, BRUCE &rft.date=2021&rft.coverage=westlimit=147.3859956; southlimit=-54.7236529; eastlimit=158.9606394; northlimit=-42.9491591&rft.coverage=westlimit=147.3859956; southlimit=-54.7236529; eastlimit=158.9606394; northlimit=-42.9491591&rft_rights=These data are not yet publicly available for download.&rft_rights=Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/legalcode&rft_rights=This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4556_group_specific_eDNA_V4_2018 when using these data.&rft_rights=This metadata record is publicly available.&rft_subject=biota&rft_subject=oceans&rft_subject=EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/VERTEBRATES > FISH&rft_subject=EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/INVERTEBRATES > ARTHROPODS > CRUSTACEANS&rft_subject=PCR&rft_subject=MISEQ&rft_subject=ENVIRONMENTAL DNA&rft_subject=METABARCODING&rft_subject=BIODIVERSITY MONITORING&rft_subject=ADS > Automated DNA Sequencer&rft_subject=R/V AA > R/V Aurora Australis&rft_subject=LABORATORY&rft_subject=AMD/AU&rft_subject=AMD&rft_subject=CEOS&rft_subject=OCEAN > SOUTHERN OCEAN > MACQUARIE ISLAND&rft_subject=OCEAN > SOUTHERN OCEAN&rft_subject=GEOGRAPHIC REGION > POLAR&rft.type=dataset&rft.language=English Access the data
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Attribution 4.0 International (CC BY 4.0)
https://creativecommons.org/licenses/by/4.0/legalcode

These data are not yet publicly available for download.

This data set conforms to the CCBY Attribution License (http://creativecommons.org/licenses/by/4.0/).

Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4556_group_specific_eDNA_V4_2018 when using these data.

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Full description

In March 2018, 23 environmental DNA (eDNA) samples (2 L of filtered seawater) were collected between Hobart, Tasmania and subantarctic Macquarie Island. These samples were processed using six different genetic metabarcoding markers targeting different taxonomic groups within the metazoan clade: A broad cytochrome c oxidase subunit I (COI) marker targeting all metazoans, and five different 16S markers targeting fish, cephalopods and crustaceans (one degenerate marker), fish (two markers of different lengths), cephalopods (one marker) and crustaceans (one marker). The aim of this study was to identify an ideal set of molecular markers to identify as many metazoan species as possible from small environmental samples, with a particular focus on vertebrates, crustaceans and cephalopods.

The data and methods are described in the word file "V4 2018 eDNA group specific markers.docx", results are summarised in the excel file "Marker.detection.xlsx" and additional sample information is in the excel files "2018_11_07_eDNA-sample-info.xls" and "sample.map.csv". Each genetic marker used in this study has its own folder, containing the raw FASTQ sequencing data, the processed FASTA sequencing data, the bioinformatics processing pipeline, the zOTU fasta file, BLAST output, MEGAN output and curated zOTU table. For further explanations please refer to the word file "V4 2018 eDNA group specific markers.docx".

Lineage

Progress Code: completed

Notes

Purpose
The aim of this study was to identify an ideal set of molecular markers to identify as many metazoan species as possible from small environmental samples, with a particular focus on vertebrates, crustaceans and cephalopods.

Data time period: 2018-03-09 to 2018-03-23

This dataset is part of a larger collection

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158.96064,-42.94916 158.96064,-54.72365 147.386,-54.72365 147.386,-42.94916 158.96064,-42.94916

153.1733175,-48.836406

text: westlimit=147.3859956; southlimit=-54.7236529; eastlimit=158.9606394; northlimit=-42.9491591

Subjects
ADS > Automated DNA Sequencer | AMD | AMD/AU | BIODIVERSITY MONITORING | CEOS | EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/INVERTEBRATES > ARTHROPODS > CRUSTACEANS | EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/VERTEBRATES > FISH | ENVIRONMENTAL DNA | GEOGRAPHIC REGION > POLAR | LABORATORY | METABARCODING | MISEQ | OCEAN > SOUTHERN OCEAN | OCEAN > SOUTHERN OCEAN > MACQUARIE ISLAND | PCR | R/V AA > R/V Aurora Australis | biota | oceans |

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Other Information
Download the dataset. (GET DATA > DIRECT DOWNLOAD)

uri : https://data.aad.gov.au/eds/5221/download

Public information for AAS project AAS_4556 (PROJECT HOME PAGE)

uri : https://projects.aad.gov.au/search_projects_results.cfm?project_no=AAS_4556

Citation reference for this metadata record and dataset. (VIEW RELATED INFORMATION)

uri : https://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4556_group_specific_eDNA_V4_2018

Identifiers
  • global : AAS_4556_group_specific_eDNA_V4_2018